Restriction map for
pF1KB9740
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AlwI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbsI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tru9I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1213 GTAGAC GT^[AC][GT]AC
AccII 190 CGCG CG^CG
AccIII 1470 TCCGGA T^CCGGA
AhdI 124 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1520 GTGCAC G^TGCAC
ApaI 330 GGGCCC GGGCC^C
ApaLI 1520 GTGCAC G^TGCAC
AspEI 124 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1056 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 529 CACGTG CAC^GTG
BbvCI 1167 CCTCAGC CCTCAGC(-5/-2)
BfaI 91 CTAG C^TAG
BglII 653 AGATCT A^GATCT
BsaAI 529 CACGTG [CT]AC^GT[GA]
BsaI 1447 GGTCTC GGTCTC(1/5)
BseAI 1470 TCCGGA T^CCGGA
BsiEI 924 CGACCG CG[GA][CT]^CG
BsmI 540 GCATTC GAATGC(1/-1)
BspEI 1470 TCCGGA T^CCGGA
BspHI 665 TCATGA T^CATGA
BstBI 41 TTCGAA TT^CGAA
BstUI 190 CGCG CG^CG
BstXI 992 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 653 AGATCT [GA]^GATC[CT]
Eam1105I 124 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 855 GAAGAG CTCTTC(1/4)
EcoNI 1299 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 855 GAAGAG CTCTTC(1/4)
MaeI 91 CTAG C^TAG
MaeII 530 ACGT A^CGT
MflI 653 AGATCT [GA]^GATC[CT]
MroI 1470 TCCGGA T^CCGGA
MspA1I 594 CAGCTG C[AC]G^C[GT]G
MvnI 190 CGCG CG^CG
NaeI 941 GCCGGC GCC^GGC
NgoMIV 941 GCCGGC G^CCGGC
NspV 41 TTCGAA TT^CGAA
PflFI 1056 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1509 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 529 CACGTG CAC^GTG
PmlI 529 CACGTG CAC^GTG
PvuII 594 CAGCTG CAG^CTG
RcaI 665 TCATGA T^CATGA
SbfI 396 CCTGCAGG CCTGCA^GG
SexAI 1088 ACCAGGT A^CC[AT]GGT
SfiI 481 GGCCTCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 41 TTCGAA TT^CGAA
SmlI 548 CTCAAG C^T[CT][GA]AG
SphI 317 GCATGC GCATG^C
Sse8387I 396 CCTGCAGG CCTGCA^GG
StuI 1389 AGGCCT AGG^CCT
TfiI 1325 GATTC G^A[AT]TC
Tsp509I 36 AATT ^AATT
Tth111I 1056 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1509 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 653 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 944 GGCGCC G[GA]^CG[CT]C
1345 GGCGCC
AocI 898 CCTGAGG CC^T[ACGT]AGG
1385 CCTCAGG
AspHI 673 GAGCAC G[AT]GC[AT]^C
1520 GTGCAC
BalI 342 TGGCCA TGG^CCA
890 TGGCCA
BbeI 944 GGCGCC GGCGC^C
1345 GGCGCC
BglI 293 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
482 GCCTCTATGGC
BpmI 1115 CTCCAG CTGGAG(16/14)
1124 CTCCAG
BsaHI 944 GGCGCC G[GA]^CG[CT]C
1345 GGCGCC
BsaWI 881 ACCGGA [AT]^CCGG[AT]
1470 TCCGGA
BsiHKAI 673 GAGCAC G[AT]GC[AT]^C
1520 GTGCAC
BsrFI 926 ACCGGC [GA]^CCGG[CT]
941 GCCGGC
Bsu36I 898 CCTGAGG CC^T[ACGT]AGG
1385 CCTCAGG
Cfr10I 926 ACCGGC [GA]^CCGG[CT]
941 GCCGGC
Eco81I 898 CCTGAGG CC^T[ACGT]AGG
1385 CCTCAGG
HaeII 944 GGCGCC [GA]GCGC^[CT]
1345 GGCGCC
Hin1I 944 GGCGCC G[GA]^CG[CT]C
1345 GGCGCC
KasI 944 GGCGCC G^GCGCC
1345 GGCGCC
MluNI 342 TGGCCA TGG^CCA
890 TGGCCA
MscI 342 TGGCCA TGG^CCA
890 TGGCCA
NarI 944 GGCGCC GG^CGCC
1345 GGCGCC
NspI 258 GCATGT [GA]CATG^[CT]
317 GCATGC
SfoI 944 GGCGCC GGC^GCC
1345 GGCGCC
SmaI 118 CCCGGG CCC^GGG
453 CCCGGG
XcmI 381 CCAGCCCTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
995 CCACAGTGGCCGTGG
XmaCI 118 CCCGGG C^CCGGG
453 CCCGGG
XmaI 118 CCCGGG C^CCGGG
453 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I