Restriction map for
pF1KB9744
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MseI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1519 GACGTC GACGT^C
AccIII 479 TCCGGA T^CCGGA
AfaI 84 GTAC GT^AC
Alw44I 769 GTGCAC G^TGCAC
AocI 1584 CCTGAGG CC^T[ACGT]AGG
ApaLI 769 GTGCAC G^TGCAC
AspI 1285 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 471 GGATCC G^GATCC
BbeI 1257 GGCGCC GGCGC^C
BfaI 1240 CTAG C^TAG
BsaAI 1504 CACGTA [CT]AC^GT[GA]
BseAI 479 TCCGGA T^CCGGA
BsgI 199 GTGCAG GTGCAG(16/14)
BsiEI 735 CGGCCG CG[GA][CT]^CG
BspEI 479 TCCGGA T^CCGGA
Bsu36I 1584 CCTGAGG CC^T[ACGT]AGG
DraIII 418 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 735 CGGCCG C^GGCCG
EclXI 735 CGGCCG C^GGCCG
Eco52I 735 CGGCCG C^GGCCG
Eco81I 1584 CCTGAGG CC^T[ACGT]AGG
FseI 232 GGCCGGCC GGCCGG^CC
HgaI 879 GACGC GACGC(5/10)
KasI 1257 GGCGCC G^GCGCC
MaeI 1240 CTAG C^TAG
MroI 479 TCCGGA T^CCGGA
NarI 1257 GGCGCC GG^CGCC
NheI 1239 GCTAGC G^CTAGC
NspI 1091 GCATGC [GA]CATG^[CT]
PflFI 1285 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 396 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 264 CTGCAG CTGCA^G
RsaI 84 GTAC GT^AC
SacI 759 GAGCTC GAGCT^C
SexAI 86 ACCTGGT A^CC[AT]GGT
SfcI 264 CTGCAG C^T[GA][CT]AG
SfiI 1194 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1257 GGCGCC GGC^GCC
SmlI 283 CTCAAG C^T[CT][GA]AG
SphI 1091 GCATGC GCATG^C
TaqI 1499 TCGA T^CGA
Tth111I 1285 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1499 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 100 CGCG CG^CG
409 CGCG
AcyI 1257 GGCGCC G[GA]^CG[CT]C
1519 GACGTC
ApaI 581 GGGCCC GGGCC^C
975 GGGCCC
BcgI 1518 CGACGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1490 GCAGCCTCATCG
BlpI 9 GCTGAGC GC^T[ACGT]AGC
1050 GCTGAGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
1050 GCTGAGC
BsaHI 1257 GGCGCC G[GA]^CG[CT]C
1519 GACGTC
BsaI 412 GAGACC GGTCTC(1/5)
1105 GGTCTC
BsrFI 233 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
BstUI 100 CGCG CG^CG
409 CGCG
CelII 9 GCTGAGC GC^T[ACGT]AGC
1050 GCTGAGC
Cfr10I 233 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
DsaI 680 CCATGG C^C[GA][CT]GG
990 CCATGG
EcoNI 543 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
708 CCTAATGAAGG
Hin1I 1257 GGCGCC G[GA]^CG[CT]C
1519 GACGTC
MvnI 100 CGCG CG^CG
409 CGCG
NaeI 233 GCCGGC GCC^GGC
269 GCCGGC
NcoI 680 CCATGG C^CATGG
990 CCATGG
NgoMIV 233 GCCGGC G^CCGGC
269 GCCGGC
PvuII 123 CAGCTG CAG^CTG
750 CAGCTG
SmaI 401 CCCGGG CCC^GGG
1096 CCCGGG
StuI 627 AGGCCT AGG^CCT
659 AGGCCT
XmaCI 401 CCCGGG C^CCGGG
1096 CCCGGG
XmaI 401 CCCGGG C^CCGGG
1096 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
Van91I XhoII