Restriction map for
pF1KB9749
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
MaeI MamI MfeI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 189 ACGCGT A^C[GA][CT]GT
AlwI 1452 GATCC GGATC(4/5)
ApaI 811 GGGCCC GGGCC^C
BbeI 729 GGCGCC GGCGC^C
BcgI 1266 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1048 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 163 CTGCAC GTGCAG(16/14)
BsiEI 856 CGGCCG CG[GA][CT]^CG
BsrBI 607 GAGCGG CCGCTC(-3/-3)
BsrDI 120 GCAATG GCAATG(2/0)
BstEII 588 GGTGACC G^GT[ACGT]ACC
BstPI 588 GGTGACC G^GT[ACGT]ACC
BstXI 644 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1451 AGATCC [GA]^GATC[CT]
EagI 856 CGGCCG C^GGCCG
EclXI 856 CGGCCG C^GGCCG
Eco52I 856 CGGCCG C^GGCCG
EcoNI 1455 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 588 GGTGACC G^GT[ACGT]ACC
FseI 857 GGCCGGCC GGCCGG^CC
KasI 729 GGCGCC G^GCGCC
KspI 108 CCGCGG CCGC^GG
MflI 1451 AGATCC [GA]^GATC[CT]
MluI 189 ACGCGT A^CGCGT
MspA1I 108 CCGCGG C[AC]G^C[GT]G
NarI 729 GGCGCC GG^CGCC
NcoI 1211 CCATGG C^CATGG
SacI 636 GAGCTC GAGCT^C
SacII 108 CCGCGG CCGC^GG
SapI 903 GAAGAGC GCTCTTC(1/4)
SfaNI 42 GCATC GCATC(5/9)
SfiI 1047 GGCCAAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 729 GGCGCC GGC^GCC
SmlI 1456 CTCAAG C^T[CT][GA]AG
TfiI 1557 GAATC G^A[AT]TC
Tsp509I 489 AATT ^AATT
TspRI 10 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 1049 CCAAAAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1451 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 547 GACGCC G[GA]^CG[CT]C
729 GGCGCC
AfaI 231 GTAC GT^AC
396 GTAC
Alw44I 12 GTGCAC G^TGCAC
259 GTGCAC
ApaLI 12 GTGCAC G^TGCAC
259 GTGCAC
AviII 56 TGCGCA TGC^GCA
1640 TGCGCA
BalI 1575 TGGCCA TGG^CCA
1670 TGGCCA
BbsI 519 GAAGAC GAAGAC(2/6)
154 GTCTTC
BpuAI 519 GAAGAC GAAGAC(2/6)
154 GTCTTC
BsaHI 547 GACGCC G[GA]^CG[CT]C
729 GGCGCC
BsaI 778 GAGACC GGTCTC(1/5)
1630 GAGACC
BsaWI 392 TCCGGT [AT]^CCGG[AT]
1508 ACCGGA
BspMI 347 ACCTGC ACCTGC(4/8)
1223 ACCTGC
DpnII 1452 GATC ^GATC
1563 GATC
DraIII 136 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
583 CACAAGGTG
FspI 56 TGCGCA TGC^GCA
1640 TGCGCA
HgaI 188 GACGC GACGC(5/10)
547 GACGC
Hin1I 547 GACGCC G[GA]^CG[CT]C
729 GGCGCC
MaeII 796 ACGT A^CGT
1538 ACGT
MboI 1452 GATC ^GATC
1563 GATC
MluNI 1575 TGGCCA TGG^CCA
1670 TGGCCA
MscI 1575 TGGCCA TGG^CCA
1670 TGGCCA
MslI 252 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1579 CACTTCAATG
NdeII 1452 GATC ^GATC
1563 GATC
RsaI 231 GTAC GT^AC
396 GTAC
Sau3AI 1452 GATC ^GATC
1563 GATC
StuI 1091 AGGCCT AGG^CCT
1478 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AhdI AluI AspEI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI Eam1105I EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PleI PpuMI PstI
Sau96I ScrFI SfcI SmaI StyI
TaqI TseI Tsp45I TthHB8I XmaCI
XmaI