Restriction map for
pF1KB9751
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BbvI BcgI BciVI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI Fnu4HI FseI FspI HaeIII
HgaI HpaI ItaI KspI MaeII
MamI MfeI MluI MluNI MscI
MspA1I MunI MvnI MwoI NaeI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfaNI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI TseI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 900 GGTACC G^GTACC
AccIII 862 TCCGGA T^CCGGA
AcyI 220 GGCGCC G[GA]^CG[CT]C
Aor51HI 1274 AGCGCT AGC^GCT
Asp718I 900 GGTACC G^GTACC
AvaII 1638 GGTCC G^G[AT]CC
BbeI 220 GGCGCC GGCGC^C
BclI 1773 TGATCA T^GATCA
BfaI 947 CTAG C^TAG
BlpI 1697 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1697 GCTCAGC GC^T[ACGT]AGC
BsaHI 220 GGCGCC G[GA]^CG[CT]C
BsaWI 862 TCCGGA [AT]^CCGG[AT]
BseAI 862 TCCGGA T^CCGGA
BseRI 43 GAGGAG GAGGAG(10/8)
BsmFI 265 GGGAC GGGAC(10/14)
BspEI 862 TCCGGA T^CCGGA
BspHI 1584 TCATGA T^CATGA
CelII 1697 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1638 GGTCC G^G[ACGT]CC
DraI 1072 TTTAAA TTT^AAA
DraIII 695 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 530 CCATGG C^C[GA][CT]GG
Eco47III 1274 AGCGCT AGC^GCT
EcoT22I 917 ATGCAT ATGCA^T
FbaI 1773 TGATCA T^GATCA
HapII 863 CCGG C^CGG
Hin1I 220 GGCGCC G[GA]^CG[CT]C
HincII 838 GTTGAC GT[CT]^[GA]AC
HindII 838 GTTGAC GT[CT]^[GA]AC
HindIII 1450 AAGCTT A^AGCTT
HpaII 863 CCGG C^CGG
KasI 220 GGCGCC G^GCGCC
KpnI 900 GGTACC GGTAC^C
MaeI 947 CTAG C^TAG
MroI 862 TCCGGA T^CCGGA
MspI 863 CCGG C^CGG
NarI 220 GGCGCC GG^CGCC
NcoI 530 CCATGG C^CATGG
NsiI 917 ATGCAT ATGCA^T
PflMI 340 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1348 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1584 TCATGA T^CATGA
SapI 1702 GCTCTTC GCTCTTC(1/4)
Sau96I 1638 GGTCC G^G[ACGT]CC
SfcI 81 CTACAG C^T[GA][CT]AG
SfoI 220 GGCGCC GGC^GCC
SmlI 440 CTCAAG C^T[CT][GA]AG
SphI 760 GCATGC GCATG^C
SspI 1757 AATATT AAT^ATT
Van91I 340 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 858 GAGCTC G[AT]GC[AT]^C
1191 GAGCTC
BanI 220 GGCGCC G^G[CT][GA]CC
900 GGTACC
BanII 858 GAGCTC G[GA]GC[CT]^C
1191 GAGCTC
BglII 941 AGATCT A^GATCT
1365 AGATCT
BmyI 858 GAGCTC G[AGT]GC[ACT]^C
1191 GAGCTC
BpmI 725 CTGGAG CTGGAG(16/14)
1478 CTGGAG
BsiHKAI 858 GAGCTC G[AT]GC[AT]^C
1191 GAGCTC
BsmAI 1047 GAGAC GTCTC(1/5)
380 GTCTC
Bsp1286I 858 GAGCTC G[AGT]GC[ACT]^C
1191 GAGCTC
BstBI 1202 TTCGAA TT^CGAA
1706 TTCGAA
BstYI 941 AGATCT [GA]^GATC[CT]
1365 AGATCT
Cac8I 217 GCTGGC GC[ACGT]^[ACGT]GC
760 GCATGC
CfoI 221 GCGC GCG^C
1275 GCGC
EcoRI 1205 GAATTC G^AATTC
1709 GAATTC
EcoT14I 412 CCTTGG C^C[AT][AT]GG
530 CCATGG
HaeII 220 GGCGCC [GA]GCGC^[CT]
1274 AGCGCT
HhaI 221 GCGC GCG^C
1275 GCGC
HinP1I 221 GCGC G^CGC
1275 GCGC
HphI 606 GGTGA GGTGA(8/7)
1041 TCACC
MflI 941 AGATCT [GA]^GATC[CT]
1365 AGATCT
MslI 1154 CATATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1585 CATGATTGTG
NspI 760 GCATGC [GA]CATG^[CT]
919 GCATGT
NspV 1202 TTCGAA TT^CGAA
1706 TTCGAA
SacI 858 GAGCTC GAGCT^C
1191 GAGCTC
ScaI 650 AGTACT AGT^ACT
1113 AGTACT
SfuI 1202 TTCGAA TT^CGAA
1706 TTCGAA
StyI 412 CCTTGG C^C[AT][AT]GG
530 CCATGG
TaqI 1203 TCGA T^CGA
1707 TCGA
TthHB8I 1203 TCGA T^CGA
1707 TCGA
XhoII 941 AGATCT [GA]^GATC[CT]
1365 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbsI BpuAI BsaJI BsiYI BslI
BsmI BsrI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRII FokI
HinfI Ksp632I MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
TfiI Tru9I Tsp45I Tsp509I TspRI
XmnI