################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BmyI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 128 GACGCC G[GA]^CG[CT]C AflIII 823 ACATGT A^C[GA][CT]GT AseI 1032 ATTAAT AT^TAAT AsnI 1032 ATTAAT AT^TAAT AvaII 1319 GGTCC G^G[AT]CC BbsI 352 GAAGAC GAAGAC(2/6) BbvCI 762 CCTCAGC CCTCAGC(-5/-2) BfaI 1058 CTAG C^TAG BglII 842 AGATCT A^GATCT BlpI 909 GCTTAGC GC^T[ACGT]AGC Bpu1102I 909 GCTTAGC GC^T[ACGT]AGC BpuAI 352 GAAGAC GAAGAC(2/6) BsaHI 128 GACGCC G[GA]^CG[CT]C BsgI 1835 GTGCAG GTGCAG(16/14) BspHI 903 TCATGA T^CATGA BspLU11I 823 ACATGT A^CATGT BspMI 167 ACCTGC ACCTGC(4/8) BstBI 184 TTCGAA TT^CGAA BstYI 842 AGATCT [GA]^GATC[CT] CelII 909 GCTTAGC GC^T[ACGT]AGC DpnII 843 GATC ^GATC DsaI 235 CCATGG C^C[GA][CT]GG EcoNI 132 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 235 CCATGG C^C[AT][AT]GG Hin1I 128 GACGCC G[GA]^CG[CT]C MaeI 1058 CTAG C^TAG MboI 843 GATC ^GATC MflI 842 AGATCT [GA]^GATC[CT] NcoI 235 CCATGG C^CATGG NdeI 990 CATATG CA^TATG NdeII 843 GATC ^GATC NlaIV 231 GGGGCC GG[ACGT]^[ACGT]CC NspI 823 ACATGT [GA]CATG^[CT] NspV 184 TTCGAA TT^CGAA PpuMI 1318 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 1032 ATTAAT AT^TAAT RcaI 903 TCATGA T^CATGA Sau3AI 843 GATC ^GATC SfuI 184 TTCGAA TT^CGAA StyI 235 CCATGG C^C[AT][AT]GG TaqI 185 TCGA T^CGA TthHB8I 185 TCGA T^CGA XhoII 842 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BsaJI 28 CCAGGG C^C[ACGT][ACGT]GG 235 CCATGG BsmAI 1233 GAGAC GTCTC(1/5) 1554 GTCTC BsmI 1159 GAATGC GAATGC(1/-1) 1411 GAATGC BsrDI 632 GCAATG GCAATG(2/0) 1079 GCAATG Cac8I 3 GCCAGC GC[ACGT]^[ACGT]GC 1344 GCAGGC DraII 1318 AGGTCCT [GA]G^G[ACGT]CC[CT] 1681 AGGGCCT EarI 690 CTCTTC CTCTTC(1/4) 588 GAAGAG EcoO109I 1318 AGGTCCT [GA]G^G[ACGT]CC[CT] 1681 AGGGCCT HgaI 16 GACGC GACGC(5/10) 128 GACGC Ksp632I 690 CTCTTC CTCTTC(1/4) 588 GAAGAG MaeII 573 ACGT A^CGT 741 ACGT PleI 115 GACTC GAGTC(4/5) 450 GAGTC SfcI 159 CTACAG C^T[GA][CT]AG 1730 CTACAG SspI 477 AATATT AAT^ATT 1972 AATATT TfiI 367 GATTC G^A[AT]TC 577 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AcsI AfaI AluI AlwNI ApoI Asp700I BbvI BpmI BseRI BsiYI BslI BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRI EcoRII EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MslI MvaI NlaIII NsiI RsaI Sau96I ScrFI SfaNI StuI Tru9I TseI Tsp45I Tsp509I TspRI XmnI