Restriction map for pF1KB9755
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AflII       AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BmyI        BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI          128  GACGCC               G[GA]^CG[CT]C
AflIII        823  ACATGT               A^C[GA][CT]GT
AseI         1032  ATTAAT               AT^TAAT
AsnI         1032  ATTAAT               AT^TAAT
AvaII        1319  GGTCC                G^G[AT]CC
BbsI          352  GAAGAC               GAAGAC(2/6)
BbvCI         762  CCTCAGC              CCTCAGC(-5/-2)
BfaI         1058  CTAG                 C^TAG
BglII         842  AGATCT               A^GATCT
BlpI          909  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      909  GCTTAGC              GC^T[ACGT]AGC
BpuAI         352  GAAGAC               GAAGAC(2/6)
BsaHI         128  GACGCC               G[GA]^CG[CT]C
BsgI         1835  GTGCAG               GTGCAG(16/14)
BspHI         903  TCATGA               T^CATGA
BspLU11I      823  ACATGT               A^CATGT
BspMI         167  ACCTGC               ACCTGC(4/8)
BstBI         184  TTCGAA               TT^CGAA
BstYI         842  AGATCT               [GA]^GATC[CT]
CelII         909  GCTTAGC              GC^T[ACGT]AGC
DpnII         843  GATC                 ^GATC
DsaI          235  CCATGG               C^C[GA][CT]GG
EcoNI         132  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       235  CCATGG               C^C[AT][AT]GG
Hin1I         128  GACGCC               G[GA]^CG[CT]C
MaeI         1058  CTAG                 C^TAG
MboI          843  GATC                 ^GATC
MflI          842  AGATCT               [GA]^GATC[CT]
NcoI          235  CCATGG               C^CATGG
NdeI          990  CATATG               CA^TATG
NdeII         843  GATC                 ^GATC
NlaIV         231  GGGGCC               GG[ACGT]^[ACGT]CC
NspI          823  ACATGT               [GA]CATG^[CT]
NspV          184  TTCGAA               TT^CGAA
PpuMI        1318  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1032  ATTAAT               AT^TAAT
RcaI          903  TCATGA               T^CATGA
Sau3AI        843  GATC                 ^GATC
SfuI          184  TTCGAA               TT^CGAA
StyI          235  CCATGG               C^C[AT][AT]GG
TaqI          185  TCGA                 T^CGA
TthHB8I       185  TCGA                 T^CGA
XhoII         842  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BsaJI          28  CCAGGG               C^C[ACGT][ACGT]GG
              235  CCATGG              
BsmAI        1233  GAGAC                GTCTC(1/5)
             1554  GTCTC               
BsmI         1159  GAATGC               GAATGC(1/-1)
             1411  GAATGC              
BsrDI         632  GCAATG               GCAATG(2/0)
             1079  GCAATG              
Cac8I           3  GCCAGC               GC[ACGT]^[ACGT]GC
             1344  GCAGGC              
DraII        1318  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1681  AGGGCCT             
EarI          690  CTCTTC               CTCTTC(1/4)
              588  GAAGAG              
EcoO109I     1318  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1681  AGGGCCT             
HgaI           16  GACGC                GACGC(5/10)
              128  GACGC               
Ksp632I       690  CTCTTC               CTCTTC(1/4)
              588  GAAGAG              
MaeII         573  ACGT                 A^CGT
              741  ACGT                
PleI          115  GACTC                GAGTC(4/5)
              450  GAGTC               
SfcI          159  CTACAG               C^T[GA][CT]AG
             1730  CTACAG              
SspI          477  AATATT               AAT^ATT
             1972  AATATT              
TfiI          367  GATTC                G^A[AT]TC
              577  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwNI       ApoI        
Asp700I     BbvI        BpmI        BseRI       BsiYI       
BslI        BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        EcoRI       EcoRII      EcoT22I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeIII      MboII       MnlI        MseI        
MslI        MvaI        NlaIII      NsiI        RsaI        
Sau96I      ScrFI       SfaNI       StuI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XmnI        
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