##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BmyI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 128 GACGCC G[GA]^CG[CT]C
AflIII 823 ACATGT A^C[GA][CT]GT
AseI 1032 ATTAAT AT^TAAT
AsnI 1032 ATTAAT AT^TAAT
AvaII 1319 GGTCC G^G[AT]CC
BbsI 352 GAAGAC GAAGAC(2/6)
BbvCI 762 CCTCAGC CCTCAGC(-5/-2)
BfaI 1058 CTAG C^TAG
BglII 842 AGATCT A^GATCT
BlpI 909 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 909 GCTTAGC GC^T[ACGT]AGC
BpuAI 352 GAAGAC GAAGAC(2/6)
BsaHI 128 GACGCC G[GA]^CG[CT]C
BsgI 1835 GTGCAG GTGCAG(16/14)
BspHI 903 TCATGA T^CATGA
BspLU11I 823 ACATGT A^CATGT
BspMI 167 ACCTGC ACCTGC(4/8)
BstBI 184 TTCGAA TT^CGAA
BstYI 842 AGATCT [GA]^GATC[CT]
CelII 909 GCTTAGC GC^T[ACGT]AGC
DpnII 843 GATC ^GATC
DsaI 235 CCATGG C^C[GA][CT]GG
EcoNI 132 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 235 CCATGG C^C[AT][AT]GG
Hin1I 128 GACGCC G[GA]^CG[CT]C
MaeI 1058 CTAG C^TAG
MboI 843 GATC ^GATC
MflI 842 AGATCT [GA]^GATC[CT]
NcoI 235 CCATGG C^CATGG
NdeI 990 CATATG CA^TATG
NdeII 843 GATC ^GATC
NlaIV 231 GGGGCC GG[ACGT]^[ACGT]CC
NspI 823 ACATGT [GA]CATG^[CT]
NspV 184 TTCGAA TT^CGAA
PpuMI 1318 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1032 ATTAAT AT^TAAT
RcaI 903 TCATGA T^CATGA
Sau3AI 843 GATC ^GATC
SfuI 184 TTCGAA TT^CGAA
StyI 235 CCATGG C^C[AT][AT]GG
TaqI 185 TCGA T^CGA
TthHB8I 185 TCGA T^CGA
XhoII 842 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsaJI 28 CCAGGG C^C[ACGT][ACGT]GG
235 CCATGG
BsmAI 1233 GAGAC GTCTC(1/5)
1554 GTCTC
BsmI 1159 GAATGC GAATGC(1/-1)
1411 GAATGC
BsrDI 632 GCAATG GCAATG(2/0)
1079 GCAATG
Cac8I 3 GCCAGC GC[ACGT]^[ACGT]GC
1344 GCAGGC
DraII 1318 AGGTCCT [GA]G^G[ACGT]CC[CT]
1681 AGGGCCT
EarI 690 CTCTTC CTCTTC(1/4)
588 GAAGAG
EcoO109I 1318 AGGTCCT [GA]G^G[ACGT]CC[CT]
1681 AGGGCCT
HgaI 16 GACGC GACGC(5/10)
128 GACGC
Ksp632I 690 CTCTTC CTCTTC(1/4)
588 GAAGAG
MaeII 573 ACGT A^CGT
741 ACGT
PleI 115 GACTC GAGTC(4/5)
450 GAGTC
SfcI 159 CTACAG C^T[GA][CT]AG
1730 CTACAG
SspI 477 AATATT AAT^ATT
1972 AATATT
TfiI 367 GATTC G^A[AT]TC
577 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
Asp700I BbvI BpmI BseRI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cfr13I DdeI EcoRI EcoRII EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MseI
MslI MvaI NlaIII NsiI RsaI
Sau96I ScrFI SfaNI StuI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI