Restriction map for
pF1KB9756
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssSI BstBI CelII ClaI CpoI
DraI DraIII DrdI EcoNI EcoRI
EcoRV EcoT22I FseI FspI HindIII
KpnI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfaNI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 145 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 451 CCTCAGG CC^T[ACGT]AGG
AseI 2044 ATTAAT AT^TAAT
AsnI 2044 ATTAAT AT^TAAT
AspEI 145 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 510 GGCGCC GGCGC^C
BsiEI 306 CGGCCG CG[GA][CT]^CG
Bsp1407I 1382 TGTACA T^GTACA
BspMI 38 ACCTGC ACCTGC(4/8)
BsrGI 1382 TGTACA T^GTACA
Bst1107I 1960 GTATAC GTA^TAC
BstAPI 1291 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1002 GGTGACC G^GT[ACGT]ACC
BstPI 1002 GGTGACC G^GT[ACGT]ACC
BstXI 1626 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1960 GTATAC GTA^TAC
Bsu36I 451 CCTCAGG CC^T[ACGT]AGG
EagI 306 CGGCCG C^GGCCG
Eam1105I 145 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 306 CGGCCG C^GGCCG
Eco52I 306 CGGCCG C^GGCCG
Eco81I 451 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1002 GGTGACC G^GT[ACGT]ACC
HpaI 1407 GTTAAC GTT^AAC
KasI 510 GGCGCC G^GCGCC
MaeII 803 ACGT A^CGT
NarI 510 GGCGCC GG^CGCC
NdeI 1697 CATATG CA^TATG
PshBI 2044 ATTAAT AT^TAAT
PstI 353 CTGCAG CTGCA^G
PvuII 1814 CAGCTG CAG^CTG
SapI 1901 GCTCTTC GCTCTTC(1/4)
SfcI 353 CTGCAG C^T[GA][CT]AG
SfiI 222 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 510 GGCGCC GGC^GCC
SspBI 1382 TGTACA T^GTACA
TaqI 1788 TCGA T^CGA
TthHB8I 1788 TCGA T^CGA
XbaI 1938 TCTAGA T^CTAGA
XcmI 495 CCAGGCGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 805 GTCTAC GT^[AC][GT]AC
1960 GTATAC
AcsI 835 AAATTC [GA]^AATT[CT]
2016 AAATTC
AcyI 250 GACGCC G[GA]^CG[CT]C
510 GGCGCC
AlwI 1436 GATCC GGATC(4/5)
748 GGATC
AlwNI 782 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1292 CAGTGACTG
Aor51HI 94 AGCGCT AGC^GCT
271 AGCGCT
ApaI 221 GGGCCC GGGCC^C
295 GGGCCC
ApoI 835 AAATTC [GA]^AATT[CT]
2016 AAATTC
AspHI 27 GAGCTC G[AT]GC[AT]^C
417 GAGCTC
AvaI 607 CCCGGG C^[CT]CG[GA]G
681 CCCGGG
BanI 510 GGCGCC G^G[CT][GA]CC
685 GGCACC
BclI 860 TGATCA T^GATCA
1335 TGATCA
BsaHI 250 GACGCC G[GA]^CG[CT]C
510 GGCGCC
BsiHKAI 27 GAGCTC G[AT]GC[AT]^C
417 GAGCTC
BsoBI 607 CCCGGG C^[CT]CG[GA]G
681 CCCGGG
BsrFI 634 GCCGGT [GA]^CCGG[CT]
818 ACCGGC
BstYI 748 GGATCT [GA]^GATC[CT]
1435 AGATCC
Cfr10I 634 GCCGGT [GA]^CCGG[CT]
818 ACCGGC
Eco47III 94 AGCGCT AGC^GCT
271 AGCGCT
FbaI 860 TGATCA T^GATCA
1335 TGATCA
Hin1I 250 GACGCC G[GA]^CG[CT]C
510 GGCGCC
HincII 1407 GTTAAC GT[CT]^[GA]AC
1498 GTCAAC
HindII 1407 GTTAAC GT[CT]^[GA]AC
1498 GTCAAC
MflI 748 GGATCT [GA]^GATC[CT]
1435 AGATCC
NcoI 404 CCATGG C^CATGG
1825 CCATGG
NspI 884 GCATGT [GA]CATG^[CT]
1028 ACATGC
PleI 917 GACTC GAGTC(4/5)
1060 GAGTC
SacI 27 GAGCTC GAGCT^C
417 GAGCTC
SmaI 607 CCCGGG CCC^GGG
681 CCCGGG
SmlI 897 CTTGAG C^T[CT][GA]AG
1511 CTCAAG
XhoII 748 GGATCT [GA]^GATC[CT]
1435 AGATCC
XmaCI 607 CCCGGG C^CCGGG
681 CCCGGG
XmaI 607 CCCGGG C^CCGGG
681 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BalI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrDI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI RsaI
SacII Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I