Restriction map for
pF1KB9758
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanII BciVI
BclI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1344 CGCG CG^CG
AcyI 1321 GGCGCC G[GA]^CG[CT]C
AflIII 1483 ACATGT A^C[GA][CT]GT
Alw44I 1108 GTGCAC G^TGCAC
AlwNI 851 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1260 AGCGCT AGC^GCT
ApaLI 1108 GTGCAC G^TGCAC
AspHI 1108 GTGCAC G[AT]GC[AT]^C
AvaII 786 GGACC G^G[AT]CC
BamHI 1543 GGATCC G^GATCC
BbeI 1321 GGCGCC GGCGC^C
BbvCI 751 GCTGAGG CCTCAGC(-5/-2)
BcgI 63 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 795 CCCGG CC^[GC]GG
BlpI 2067 GCTTAGC GC^T[ACGT]AGC
BpmI 1684 CTCCAG CTGGAG(16/14)
Bpu1102I 2067 GCTTAGC GC^T[ACGT]AGC
BsaHI 1321 GGCGCC G[GA]^CG[CT]C
BsiHKAI 1108 GTGCAC G[AT]GC[AT]^C
BsmI 323 GAATGC GAATGC(1/-1)
BspLU11I 1483 ACATGT A^CATGT
BsrBI 1213 GAGCGG CCGCTC(-3/-3)
BsrFI 1295 ACCGGC [GA]^CCGG[CT]
BssSI 1273 CACGAG CACGAG(-5/-1)
BstEII 82 GGTCACC G^GT[ACGT]ACC
BstPI 82 GGTCACC G^GT[ACGT]ACC
BstUI 1344 CGCG CG^CG
BstXI 1028 CCACCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2067 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1295 ACCGGC [GA]^CCGG[CT]
DraI 1555 TTTAAA TTT^AAA
DraII 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 94 GACGTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1260 AGCGCT AGC^GCT
EcoO109I 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 82 GGTCACC G^GT[ACGT]ACC
EcoT22I 61 ATGCAT ATGCA^T
FauI 1145 GCGGG CCCGC(4/6)
Hin1I 1321 GGCGCC G[GA]^CG[CT]C
HindIII 1897 AAGCTT A^AGCTT
KasI 1321 GGCGCC G^GCGCC
MvnI 1344 CGCG CG^CG
NarI 1321 GGCGCC GG^CGCC
NciI 795 CCCGG CC^[GC]GG
NcoI 1804 CCATGG C^CATGG
NsiI 61 ATGCAT ATGCA^T
PshAI 222 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1321 GGCGCC GGC^GCC
TaqI 2095 TCGA T^CGA
TthHB8I 2095 TCGA T^CGA
XcmI 488 CCAGGGCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 229 GTCTAC GT^[AC][GT]AC
280 GTCTAC
BanI 1217 GGCACC G^G[CT][GA]CC
1321 GGCGCC
BbrPI 1105 CACGTG CAC^GTG
1183 CACGTG
BbsI 725 GAAGAC GAAGAC(2/6)
576 GTCTTC
BmyI 807 GGGCAC G[AGT]GC[ACT]^C
1108 GTGCAC
BpuAI 725 GAAGAC GAAGAC(2/6)
576 GTCTTC
BsaAI 1105 CACGTG [CT]AC^GT[GA]
1183 CACGTG
BsaI 1292 GAGACC GGTCTC(1/5)
526 GGTCTC
Bsp1286I 807 GGGCAC G[AGT]GC[ACT]^C
1108 GTGCAC
BsrDI 566 GCAATG GCAATG(2/0)
1730 GCAATG
BstYI 701 AGATCC [GA]^GATC[CT]
1543 GGATCC
DraIII 1018 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1311 CACCGAGTG
EcoRI 1364 GAATTC G^AATTC
1593 GAATTC
HgaI 1353 GACGC GACGC(5/10)
1632 GCGTC
MflI 701 AGATCC [GA]^GATC[CT]
1543 GGATCC
PflMI 1017 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCACAACCTGG
PmaCI 1105 CACGTG CAC^GTG
1183 CACGTG
PmlI 1105 CACGTG CAC^GTG
1183 CACGTG
PstI 1189 CTGCAG CTGCA^G
1952 CTGCAG
ScaI 405 AGTACT AGT^ACT
1511 AGTACT
SmlI 271 CTTGAG C^T[CT][GA]AG
1348 CTCAAG
Van91I 1017 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCACAACCTGG
XhoII 701 AGATCC [GA]^GATC[CT]
1543 GGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BalI BbvI BfaI
BglI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BspMI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI