Restriction map for
pF1KB9762
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# NO CUT ENZYMES ( 103 enzymes) #
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AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsgI
BsiWI BsmI BspDI BspHI BspMI
BsrBI BsrDI BssSI Bst1107I BstAPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MscI MunI
NarI NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1963 GACGTC GACGT^C
Acc65I 1149 GGTACC G^GTACC
AclI 148 AACGTT AA^CGTT
AflIII 737 ACATGT A^C[GA][CT]GT
Alw44I 733 GTGCAC G^TGCAC
ApaLI 733 GTGCAC G^TGCAC
Asp700I 663 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1149 GGTACC G^GTACC
AvaI 396 CCCGGG C^[CT]CG[GA]G
BamHI 1231 GGATCC G^GATCC
BbsI 577 GAAGAC GAAGAC(2/6)
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
BglII 1533 AGATCT A^GATCT
BpuAI 577 GAAGAC GAAGAC(2/6)
BsiEI 515 CGGTCG CG[GA][CT]^CG
BsmBI 519 CGTCTC CGTCTC(1/5)
BsoBI 396 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2340 TGTACA T^GTACA
BspLU11I 737 ACATGT A^CATGT
BsrGI 2340 TGTACA T^GTACA
BssHII 434 GCGCGC G^CGCGC
BstBI 899 TTCGAA TT^CGAA
EcoRI 2289 GAATTC G^AATTC
HindIII 1507 AAGCTT A^AGCTT
KpnI 1149 GGTACC GGTAC^C
NcoI 801 CCATGG C^CATGG
NdeI 1784 CATATG CA^TATG
NspI 737 ACATGT [GA]CATG^[CT]
NspV 899 TTCGAA TT^CGAA
Psp1406I 148 AACGTT AA^CGTT
SexAI 2366 ACCTGGT A^CC[AT]GGT
SfuI 899 TTCGAA TT^CGAA
SmaI 396 CCCGGG CCC^GGG
SspBI 2340 TGTACA T^GTACA
SspI 1863 AATATT AAT^ATT
XcmI 191 CCAAGCCCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 396 CCCGGG C^CCGGG
XmaI 396 CCCGGG C^CCGGG
XmnI 663 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 754 GTCTAC GT^[AC][GT]AC
1636 GTCTAC
AlwNI 536 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2261 CAGCCACTG
AocI 49 CCTGAGG CC^T[ACGT]AGG
533 CCTCAGG
AspHI 733 GTGCAC G[AT]GC[AT]^C
1003 GAGCAC
BanI 240 GGTGCC G^G[CT][GA]CC
1149 GGTACC
BglI 128 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
334 GCCGAAGAGGC
BpmI 367 CTGGAG CTGGAG(16/14)
1457 CTGGAG
BseRI 16 GAGGAG GAGGAG(10/8)
370 GAGGAG
BsiHKAI 733 GTGCAC G[AT]GC[AT]^C
1003 GAGCAC
BstEII 426 GGTGACC G^GT[ACGT]ACC
450 GGTGACC
BstPI 426 GGTGACC G^GT[ACGT]ACC
450 GGTGACC
Bsu36I 49 CCTGAGG CC^T[ACGT]AGG
533 CCTCAGG
DraII 184 GGGACCC [GA]G^G[ACGT]CC[CT]
537 AGGTCCT
EaeI 293 TGGCCG [CT]^GGCC[GA]
1872 CGGCCA
Eco81I 49 CCTGAGG CC^T[ACGT]AGG
533 CCTCAGG
EcoO109I 184 GGGACCC [GA]G^G[ACGT]CC[CT]
537 AGGTCCT
EcoO65I 426 GGTGACC G^GT[ACGT]ACC
450 GGTGACC
EcoT14I 801 CCATGG C^C[AT][AT]GG
1356 CCTTGG
HgaI 259 GACGC GACGC(5/10)
157 GCGTC
MaeII 149 ACGT A^CGT
1964 ACGT
MslI 766 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1419 CACTGCAGTG
MspA1I 215 CCGCTG C[AC]G^C[GT]G
248 CCGCTG
PleI 285 GACTC GAGTC(4/5)
934 GACTC
PpuMI 184 GGGACCC [GA]G^G[AT]CC[CT]
537 AGGTCCT
SgrAI 261 CGCCGGCG C[GA]^CCGG[CT]G
414 CGCCGGCG
StyI 801 CCATGG C^C[AT][AT]GG
1356 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaII
BanII BbvI BcnI BfaI BmyI
BsaHI BsaI BsaJI BsaWI BseAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MroI MseI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII