Restriction map for
pF1KB9767
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
EcoO109I EcoO65I EcoRI EcoT14I FauI
FbaI FseI FspI HapII HpaII
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 2782 GGTACC G^GTACC
AccI 14 GTCGAC GT^[AC][GT]AC
AcyI 2104 GGCGTC G[GA]^CG[CT]C
AflII 1968 CTTAAG C^TTAAG
AflIII 2181 ACATGT A^C[GA][CT]GT
AlwNI 1943 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2282 CCTCAGG CC^T[ACGT]AGG
Aor51HI 242 AGCGCT AGC^GCT
AseI 1026 ATTAAT AT^TAAT
AsnI 1026 ATTAAT AT^TAAT
Asp700I 1774 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2782 GGTACC G^GTACC
AspI 931 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2782 GGTACC G^G[CT][GA]CC
BanII 2470 GAGCTC G[GA]GC[CT]^C
BfrI 1968 CTTAAG C^TTAAG
BsaHI 2104 GGCGTC G[GA]^CG[CT]C
BsaI 1731 GAGACC GGTCTC(1/5)
BsaJI 1575 CCCTGG C^C[ACGT][ACGT]GG
BspHI 1034 TCATGA T^CATGA
BspLU11I 2181 ACATGT A^CATGT
BstBI 1105 TTCGAA TT^CGAA
Bsu36I 2282 CCTCAGG CC^T[ACGT]AGG
Cac8I 849 GCTTGC GC[ACGT]^[ACGT]GC
DraI 225 TTTAAA TTT^AAA
Eco47III 242 AGCGCT AGC^GCT
Eco81I 2282 CCTCAGG CC^T[ACGT]AGG
EcoNI 2278 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1174 GATATC GAT^ATC
HaeII 242 AGCGCT [GA]GCGC^[CT]
Hin1I 2104 GGCGTC G[GA]^CG[CT]C
HindIII 1273 AAGCTT A^AGCTT
HpaI 34 GTTAAC GTT^AAC
KpnI 2782 GGTACC GGTAC^C
NdeI 869 CATATG CA^TATG
NlaIV 2782 GGTACC GG[ACGT]^[ACGT]CC
NspV 1105 TTCGAA TT^CGAA
PflFI 931 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2705 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1026 ATTAAT AT^TAAT
RcaI 1034 TCATGA T^CATGA
SacI 2470 GAGCTC GAGCT^C
SalI 14 GTCGAC G^TCGAC
SfuI 1105 TTCGAA TT^CGAA
SspI 257 AATATT AAT^ATT
SwaI 224 ATTTAAAT ATTT^AAAT
Tth111I 931 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2705 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1774 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 2470 GAGCTC G[AT]GC[AT]^C
2649 GTGCTC
BbsI 286 GAAGAC GAAGAC(2/6)
792 GAAGAC
BfaI 364 CTAG C^TAG
1844 CTAG
BmyI 2470 GAGCTC G[AGT]GC[ACT]^C
2649 GTGCTC
BpuAI 286 GAAGAC GAAGAC(2/6)
792 GAAGAC
BsiHKAI 2470 GAGCTC G[AT]GC[AT]^C
2649 GTGCTC
BsmAI 1731 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 912 GGGAC GGGAC(10/14)
1320 GTCCC
Bsp1286I 2470 GAGCTC G[AGT]GC[ACT]^C
2649 GTGCTC
Bsp1407I 1296 TGTACA T^GTACA
2379 TGTACA
BsrDI 1400 GCAATG GCAATG(2/0)
2773 GCAATG
BsrGI 1296 TGTACA T^GTACA
2379 TGTACA
CfoI 243 GCGC GCG^C
497 GCGC
HgaI 1263 GCGTC GACGC(5/10)
2105 GCGTC
HhaI 243 GCGC GCG^C
497 GCGC
HinP1I 243 GCGC G^CGC
497 GCGC
MaeI 364 CTAG C^TAG
1844 CTAG
NspI 1717 GCATGT [GA]CATG^[CT]
2181 ACATGT
PleI 914 GACTC GAGTC(4/5)
1945 GAGTC
SfcI 825 CTGTAG C^T[GA][CT]AG
2615 CTGTAG
SspBI 1296 TGTACA T^GTACA
2379 TGTACA
TaqI 15 TCGA T^CGA
1106 TCGA
TthHB8I 15 TCGA T^CGA
1106 TCGA
XbaI 363 TCTAGA T^CTAGA
1843 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BpmI BseRI BsiYI
BslI BsmI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII EcoT22I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NsiI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII