Restriction map for
pF1KB9776
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwI
AlwNI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BseAI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI DraI EagI EarI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AhdI 1783 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 967 GTGCAC G^TGCAC
Aor51HI 1868 AGCGCT AGC^GCT
ApaLI 967 GTGCAC G^TGCAC
AspEI 1783 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1022 CACGTG CAC^GTG
BbsI 644 GAAGAC GAAGAC(2/6)
BbvCI 241 GCTGAGG CCTCAGC(-5/-2)
BfaI 1201 CTAG C^TAG
BglI 1053 GCCCATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 176 AGATCT A^GATCT
BpuAI 644 GAAGAC GAAGAC(2/6)
BsaAI 1022 CACGTG [CT]AC^GT[GA]
BsaBI 1305 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1379 ACCGGA [AT]^CCGG[AT]
BsiEI 1749 CGATCG CG[GA][CT]^CG
BspHI 842 TCATGA T^CATGA
BsrDI 1037 CATTGC GCAATG(2/0)
BstEII 1483 GGTGACC G^GT[ACGT]ACC
BstPI 1483 GGTGACC G^GT[ACGT]ACC
BstXI 24 CCACTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 176 AGATCT [GA]^GATC[CT]
CpoI 1376 CGGACCG CG^G[AT]CCG
DraIII 1357 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 937 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1783 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1868 AGCGCT AGC^GCT
EcoNI 1264 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1483 GGTGACC G^GT[ACGT]ACC
HincII 1441 GTCAAC GT[CT]^[GA]AC
HindII 1441 GTCAAC GT[CT]^[GA]AC
MaeI 1201 CTAG C^TAG
MaeII 1023 ACGT A^CGT
MamI 1305 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 176 AGATCT [GA]^GATC[CT]
MseI 98 TTAA T^TAA
NaeI 1878 GCCGGC GCC^GGC
NgoMIV 1878 GCCGGC G^CCGGC
PmaCI 1022 CACGTG CAC^GTG
PmlI 1022 CACGTG CAC^GTG
PshAI 1012 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1094 CTGCAG CTGCA^G
PvuI 1749 CGATCG CGAT^CG
PvuII 239 CAGCTG CAG^CTG
RcaI 842 TCATGA T^CATGA
RsrII 1376 CGGACCG CG^G[AT]CCG
SmlI 1300 CTCAAG C^T[CT][GA]AG
SphI 459 GCATGC GCATG^C
TaqI 878 TCGA T^CGA
Tru9I 98 TTAA T^TAA
TthHB8I 878 TCGA T^CGA
XhoII 176 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 979 GGGCCC GGGCC^C
2029 GGGCCC
BbeI 1540 GGCGCC GGCGC^C
1857 GGCGCC
BpmI 1533 CTCCAG CTGGAG(16/14)
550 CTGGAG
BseRI 1164 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsmBI 40 GAGACG CGTCTC(1/5)
400 GAGACG
BspMI 1409 ACCTGC ACCTGC(4/8)
596 GCAGGT
BsrFI 1105 ACCGGC [GA]^CCGG[CT]
1878 GCCGGC
Cfr10I 1105 ACCGGC [GA]^CCGG[CT]
1878 GCCGGC
KasI 1540 GGCGCC G^GCGCC
1857 GGCGCC
KspI 1846 CCGCGG CCGC^GG
2018 CCGCGG
NarI 1540 GGCGCC GG^CGCC
1857 GGCGCC
NspI 459 GCATGC [GA]CATG^[CT]
1292 GCATGT
PpuMI 1010 GGGACCC [GA]G^G[AT]CC[CT]
1766 AGGACCC
SacII 1846 CCGCGG CCGC^GG
2018 CCGCGG
SfoI 1540 GGCGCC GGC^GCC
1857 GGCGCC
SmaI 1578 CCCGGG CCC^GGG
1650 CCCGGG
TfiI 1267 GAATC G^A[AT]TC
1810 GAATC
XcmI 351 CCACCCTCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1518 CCACGATCACGATGG
XmaCI 1578 CCCGGG C^CCGGG
1650 CCCGGG
XmaI 1578 CCCGGG C^CCGGG
1650 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BsaHI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI Van91I