################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 409 GCGG CCGC(-3/-1) AcsI 319 AAATTC [GA]^AATT[CT] AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 319 AAATTC [GA]^AATT[CT] Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C BfaI 313 CTAG C^TAG BglII 257 AGATCT A^GATCT BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C BpmI 221 CTCCAG CTGGAG(16/14) Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BsaI 82 GAGACC GGTCTC(1/5) BsgI 324 CTGCAC GTGCAG(16/14) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 82 GAGAC GTCTC(1/5) BsmI 405 GAATGC GAATGC(1/-1) Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstF5I 300 GGATG GGATG(2/0) CelII 39 GCTCAGC GC^T[ACGT]AGC FokI 300 GGATG GGATG(9/13) HaeIII 3 GGCC GG^CC MaeI 313 CTAG C^TAG MseI 553 TTAA T^TAA NlaIV 219 GGCTCC GG[ACGT]^[ACGT]CC PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 412 GACTC GAGTC(4/5) RcaI 512 TCATGA T^CATGA Tru9I 553 TTAA T^TAA Tsp509I 320 AATT ^AATT Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 249 GATCC GGATC(4/5) 518 GATCC BsaJI 185 CCCAGG C^C[ACGT][ACGT]GG 488 CCTGGG BseRI 190 GAGGAG GAGGAG(10/8) 376 GAGGAG BstYI 257 AGATCT [GA]^GATC[CT] 517 AGATCC DdeI 40 CTCAG C^T[ACGT]AG 80 CTGAG DraII 106 AGGACCT [GA]G^G[ACGT]CC[CT] 305 AGGACCT EcoO109I 106 AGGACCT [GA]G^G[ACGT]CC[CT] 305 AGGACCT MflI 257 AGATCT [GA]^GATC[CT] 517 AGATCC MspA1I 42 CAGCTG C[AC]G^C[GT]G 343 CAGCTG PpuMI 106 AGGACCT [GA]G^G[AT]CC[CT] 305 AGGACCT PvuII 42 CAGCTG CAG^CTG 343 CAGCTG SfaNI 114 GATGC GCATC(5/9) 369 GATGC XhoII 257 AGATCT [GA]^GATC[CT] 517 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI AvaII BbvI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HinfI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI TfiI TseI