Restriction map for pF1KB9785
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tsp45I      TspRI       Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          409  GCGG                 CCGC(-3/-1)
AcsI          319  AAATTC               [GA]^AATT[CT]
AlwNI         340  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          319  AAATTC               [GA]^AATT[CT]
Asp700I       432  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          37  GTGCTC               G[AT]GC[AT]^C
BfaI          313  CTAG                 C^TAG
BglII         257  AGATCT               A^GATCT
BlpI           39  GCTCAGC              GC^T[ACGT]AGC
BmyI           37  GTGCTC               G[AGT]GC[ACT]^C
BpmI          221  CTCCAG               CTGGAG(16/14)
Bpu1102I       39  GCTCAGC              GC^T[ACGT]AGC
BsaI           82  GAGACC               GGTCTC(1/5)
BsgI          324  CTGCAC               GTGCAG(16/14)
BsiHKAI        37  GTGCTC               G[AT]GC[AT]^C
BsiYI          87  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           87  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          82  GAGAC                GTCTC(1/5)
BsmI          405  GAATGC               GAATGC(1/-1)
Bsp1286I       37  GTGCTC               G[AGT]GC[ACT]^C
BspHI         512  TCATGA               T^CATGA
BsrI          207  CCAGT                ACTGG(1/-1)
BstF5I        300  GGATG                GGATG(2/0)
CelII          39  GCTCAGC              GC^T[ACGT]AGC
FokI          300  GGATG                GGATG(9/13)
HaeIII          3  GGCC                 GG^CC
MaeI          313  CTAG                 C^TAG
MseI          553  TTAA                 T^TAA
NlaIV         219  GGCTCC               GG[ACGT]^[ACGT]CC
PflMI          87  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          412  GACTC                GAGTC(4/5)
RcaI          512  TCATGA               T^CATGA
Tru9I         553  TTAA                 T^TAA
Tsp509I       320  AATT                 ^AATT
Van91I         87  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          432  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwI          249  GATCC                GGATC(4/5)
              518  GATCC               
BsaJI         185  CCCAGG               C^C[ACGT][ACGT]GG
              488  CCTGGG              
BseRI         190  GAGGAG               GAGGAG(10/8)
              376  GAGGAG              
BstYI         257  AGATCT               [GA]^GATC[CT]
              517  AGATCC              
DdeI           40  CTCAG                C^T[ACGT]AG
               80  CTGAG               
DraII         106  AGGACCT              [GA]G^G[ACGT]CC[CT]
              305  AGGACCT             
EcoO109I      106  AGGACCT              [GA]G^G[ACGT]CC[CT]
              305  AGGACCT             
MflI          257  AGATCT               [GA]^GATC[CT]
              517  AGATCC              
MspA1I         42  CAGCTG               C[AC]G^C[GT]G
              343  CAGCTG              
PpuMI         106  AGGACCT              [GA]G^G[AT]CC[CT]
              305  AGGACCT             
PvuII          42  CAGCTG               CAG^CTG
              343  CAGCTG              
SfaNI         114  GATGC                GCATC(5/9)
              369  GATGC               
XhoII         257  AGATCT               [GA]^GATC[CT]
              517  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AvaII       BbvI        BssKI       BstNI       
Cfr13I      DpnII       EcoRII      Fnu4HI      HinfI       
ItaI        MboI        MboII       MnlI        MvaI        
MwoI        NdeII       NlaIII      Sau3AI      Sau96I      
ScrFI       TfiI        TseI        
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