Restriction map for
pF1KB9787
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI Fnu4HI FseI FspI
HaeII HindIII HpaI ItaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
MvnI MwoI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
TseI Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 130 GACGTC GACGT^C
AciI 360 GCGG CCGC(-3/-1)
ApaI 370 GGGCCC GGGCC^C
AspHI 450 GTGCTC G[AT]GC[AT]^C
BanII 370 GGGCCC G[GA]GC[CT]^C
BbsI 133 GTCTTC GAAGAC(2/6)
BcnI 87 CCCGG CC^[GC]GG
BglII 566 AGATCT A^GATCT
BlpI 452 GCTCAGC GC^T[ACGT]AGC
BpmI 445 CTGGAG CTGGAG(16/14)
Bpu1102I 452 GCTCAGC GC^T[ACGT]AGC
BpuAI 133 GTCTTC GAAGAC(2/6)
BsaI 79 GAGACC GGTCTC(1/5)
BsiHKAI 450 GTGCTC G[AT]GC[AT]^C
BsmBI 525 GAGACG CGTCTC(1/5)
BsmFI 170 GGGAC GGGAC(10/14)
BspMI 480 GCAGGT ACCTGC(4/8)
BsrDI 182 GCAATG GCAATG(2/0)
BsrFI 178 GCCGGC [GA]^CCGG[CT]
BsrI 414 ACTGG ACTGG(1/-1)
BstYI 566 AGATCT [GA]^GATC[CT]
CelII 452 GCTCAGC GC^T[ACGT]AGC
Cfr10I 178 GCCGGC [GA]^CCGG[CT]
DsaI 398 CCATGG C^C[GA][CT]GG
EarI 544 CTCTTC CTCTTC(1/4)
FauI 360 GCGGG CCCGC(4/6)
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
HinfI 34 GACTC G^A[ACGT]TC
Ksp632I 544 CTCTTC CTCTTC(1/4)
MflI 566 AGATCT [GA]^GATC[CT]
MslI 120 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 358 CAGCGG C[AC]G^C[GT]G
NaeI 178 GCCGGC GCC^GGC
NciI 87 CCCGG CC^[GC]GG
NcoI 398 CCATGG C^CATGG
NgoMIV 178 GCCGGC G^CCGGC
PleI 34 GACTC GAGTC(4/5)
SapI 543 GCTCTTC GCTCTTC(1/4)
StuI 92 AGGCCT AGG^CCT
Tsp509I 303 AATT ^AATT
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 434 CCAAGGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 566 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 130 GACGTC G[GA]^CG[CT]C
187 GACGCC
BalI 254 TGGCCA TGG^CCA
341 TGGCCA
BmyI 370 GGGCCC G[AGT]GC[ACT]^C
450 GTGCTC
BsaHI 130 GACGTC G[GA]^CG[CT]C
187 GACGCC
BsiYI 327 CCCTGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
398 CCATGGAAGGG
BslI 327 CCCTGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
398 CCATGGAAGGG
BsmAI 79 GAGAC GTCTC(1/5)
525 GAGAC
Bsp1286I 370 GGGCCC G[AGT]GC[ACT]^C
450 GTGCTC
Cac8I 178 GCCGGC GC[ACGT]^[ACGT]GC
222 GCAGGC
CfoI 66 GCGC GCG^C
494 GCGC
DpnII 153 GATC ^GATC
567 GATC
EaeI 254 TGGCCA [CT]^GGCC[GA]
341 TGGCCA
EcoT14I 398 CCATGG C^C[AT][AT]GG
434 CCAAGG
HgaI 187 GACGC GACGC(5/10)
389 GCGTC
HhaI 66 GCGC GCG^C
494 GCGC
Hin1I 130 GACGTC G[GA]^CG[CT]C
187 GACGCC
HinP1I 66 GCGC G^CGC
494 GCGC
HphI 298 GGTGA GGTGA(8/7)
71 TCACC
MaeII 131 ACGT A^CGT
230 ACGT
MboI 153 GATC ^GATC
567 GATC
MluNI 254 TGGCCA TGG^CCA
341 TGGCCA
MscI 254 TGGCCA TGG^CCA
341 TGGCCA
NdeII 153 GATC ^GATC
567 GATC
NlaIII 138 CATG CATG^
399 CATG
Sau3AI 153 GATC ^GATC
567 GATC
SfaNI 146 GCATC GCATC(5/9)
200 GCATC
StyI 398 CCATGG C^C[AT][AT]GG
434 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI BsaJI BssKI BstF5I BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
FokI HaeIII HapII HpaII MboII
MnlI MspI MvaI NlaIV RsaI
Sau96I ScrFI