Restriction map for
pF1KB9788
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 454 GTCTAC GT^[AC][GT]AC
AfaI 435 GTAC GT^AC
AluI 43 AGCT AG^CT
AlwI 58 GGATC GGATC(4/5)
Aor51HI 281 AGCGCT AGC^GCT
BanII 576 GGGCTC G[GA]GC[CT]^C
BglII 257 AGATCT A^GATCT
BmyI 576 GGGCTC G[AGT]GC[ACT]^C
BpmI 355 CTGGAG CTGGAG(16/14)
Bsp1286I 576 GGGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
Cac8I 365 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 282 GCGC GCG^C
DsaI 87 CCATGG C^C[GA][CT]GG
Eco47III 281 AGCGCT AGC^GCT
EcoNI 425 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 281 AGCGCT [GA]GCGC^[CT]
HhaI 282 GCGC GCG^C
HinP1I 282 GCGC G^CGC
MslI 108 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 92 GCCTACTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 87 CCATGG C^CATGG
NspI 539 ACATGC [GA]CATG^[CT]
PflMI 186 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 512 TCATGA T^CATGA
RsaI 435 GTAC GT^AC
SfaNI 246 GATGC GCATC(5/9)
Tsp509I 405 AATT ^AATT
Van91I 186 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 318 CCAACTCCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
477 CAGCGACTG
BfaI 31 CTAG C^TAG
313 CTAG
BsaI 358 GAGACC GGTCTC(1/5)
569 GAGACC
BsgI 121 CTGCAC GTGCAG(16/14)
416 CTGCAC
BspMI 361 ACCTGC ACCTGC(4/8)
372 GCAGGT
BstYI 58 GGATCT [GA]^GATC[CT]
257 AGATCT
Cfr13I 3 GGCCC G^G[ACGT]CC
219 GGCCC
EarI 268 CTCTTC CTCTTC(1/4)
167 GAAGAG
EcoT14I 87 CCATGG C^C[AT][AT]GG
110 CCTTGG
Ksp632I 268 CTCTTC CTCTTC(1/4)
167 GAAGAG
MaeI 31 CTAG C^TAG
313 CTAG
MflI 58 GGATCT [GA]^GATC[CT]
257 AGATCT
NlaIV 179 GGTTCC GG[ACGT]^[ACGT]CC
203 GGAGCC
PstI 250 CTGCAG CTGCA^G
370 CTGCAG
Sau96I 3 GGCCC G^G[ACGT]CC
219 GGCCC
SmlI 157 CTCAAG C^T[CT][GA]AG
426 CTTGAG
StyI 87 CCATGG C^C[AT][AT]GG
110 CCTTGG
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
419 CACTG
XhoII 58 GGATCT [GA]^GATC[CT]
257 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BbvI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstNI DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
ItaI MboI MboII MnlI MvaI
NdeII NlaIII Sau3AI ScrFI SfcI
TfiI TseI