################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeII MaeIII MamI MfeI MluI MluNI MscI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 570 TCCGGA T^CCGGA AflII 409 CTTAAG C^TTAAG AlwNI 352 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 405 AGCGCT AGC^GCT AvrII 531 CCTAGG C^CTAGG BfrI 409 CTTAAG C^TTAAG BlnI 531 CCTAGG C^CTAGG BsaWI 570 TCCGGA [AT]^CCGG[AT] BseAI 570 TCCGGA T^CCGGA BsgI 118 GTGCAG GTGCAG(16/14) BsiEI 126 CGGCCG CG[GA][CT]^CG BsmFI 304 GGGAC GGGAC(10/14) BspEI 570 TCCGGA T^CCGGA BsrFI 200 ACCGGC [GA]^CCGG[CT] BstF5I 314 CATCC GGATG(2/0) BstYI 176 AGATCC [GA]^GATC[CT] Cfr10I 200 ACCGGC [GA]^CCGG[CT] DsaI 450 CCGCGG C^C[GA][CT]GG EagI 126 CGGCCG C^GGCCG EclXI 126 CGGCCG C^GGCCG Eco47III 405 AGCGCT AGC^GCT Eco52I 126 CGGCCG C^GGCCG EcoNI 132 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 531 CCTAGG C^C[AT][AT]GG FokI 314 CATCC GGATG(9/13) HgaI 204 GCGTC GACGC(5/10) HphI 144 GGTGA GGTGA(8/7) KspI 450 CCGCGG CCGC^GG MflI 176 AGATCC [GA]^GATC[CT] MroI 570 TCCGGA T^CCGGA MseI 410 TTAA T^TAA MslI 284 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 135 CATG CATG^ NotI 125 GCGGCCGC GC^GGCCGC PleI 253 GACTC GAGTC(4/5) PvuII 154 CAGCTG CAG^CTG SacII 450 CCGCGG CCGC^GG SfaNI 313 GCATC GCATC(5/9) SmaI 317 CCCGGG CCC^GGG StyI 531 CCTAGG C^C[AT][AT]GG TfiI 147 GAATC G^A[AT]TC Tru9I 410 TTAA T^TAA XhoII 176 AGATCC [GA]^GATC[CT] XmaCI 317 CCCGGG C^CCGGG XmaI 317 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### ApaI 444 GGGCCC GGGCC^C 520 GGGCCC AscI 99 GGCGCGCC GG^CGCGCC 275 GGCGCGCC AspHI 184 GAGCTC G[AT]GC[AT]^C 415 GAGCTC AvaI 317 CCCGGG C^[CT]CG[GA]G 496 CCCGAG AvaII 305 GGACC G^G[AT]CC 333 GGACC BanI 77 GGCGCC G^G[CT][GA]CC 466 GGCGCC BbeI 77 GGCGCC GGCGC^C 466 GGCGCC BsiHKAI 184 GAGCTC G[AT]GC[AT]^C 415 GAGCTC BsoBI 317 CCCGGG C^[CT]CG[GA]G 496 CCCGAG BssHII 100 GCGCGC G^CGCGC 276 GCGCGC EaeI 126 CGGCCG [CT]^GGCC[GA] 359 TGGCCG EarI 298 CTCTTC CTCTTC(1/4) 64 GAAGAG HinfI 147 GAATC G^A[ACGT]TC 253 GACTC KasI 77 GGCGCC G^GCGCC 466 GGCGCC Ksp632I 298 CTCTTC CTCTTC(1/4) 64 GAAGAG NarI 77 GGCGCC GG^CGCC 466 GGCGCC PpuMI 304 GGGACCC [GA]G^G[AT]CC[CT] 332 AGGACCT PstI 157 CTGCAG CTGCA^G 208 CTGCAG SacI 184 GAGCTC GAGCT^C 415 GAGCTC SapI 64 GAAGAGC GCTCTTC(1/4) 297 GCTCTTC SfcI 157 CTGCAG C^T[GA][CT]AG 208 CTGCAG SfoI 77 GGCGCC GGC^GCC 466 GGCGCC SmlI 187 CTCAAG C^T[CT][GA]AG 409 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcyI AluI AlwI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI TspRI