Restriction map for
pF1KB9791
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MboI MflI MluI MluNI
MroI MscI MseI MslI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI Sau3AI
SbfI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 346 CGCG CG^CG
AfaI 402 GTAC GT^AC
AlwNI 300 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 163 GGGCCC GGGCC^C
BanII 163 GGGCCC G[GA]GC[CT]^C
BcgI 366 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 146 CTAG C^TAG
BpmI 184 CTGGAG CTGGAG(16/14)
BsrDI 89 CATTGC GCAATG(2/0)
BsrFI 233 GCCGGC [GA]^CCGG[CT]
BstBI 578 TTCGAA TT^CGAA
BstF5I 30 CATCC GGATG(2/0)
BstNI 552 CCAGG CC^[AT]GG
BstUI 346 CGCG CG^CG
CfoI 345 GCGC GCG^C
Cfr10I 233 GCCGGC [GA]^CCGG[CT]
CpoI 322 CGGACCG CG^G[AT]CCG
EarI 175 CTCTTC CTCTTC(1/4)
EcoNI 180 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 552 CCAGG ^CC[AT]GG
FokI 30 CATCC GGATG(9/13)
HgaI 596 GCGTC GACGC(5/10)
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
Ksp632I 175 CTCTTC CTCTTC(1/4)
MaeI 146 CTAG C^TAG
MaeIII 310 GTGAC ^GT[ACGT]AC
MboII 176 TCTTC GAAGA(8/7)
MfeI 62 CAATTG C^AATTG
MunI 62 CAATTG C^AATTG
MvaI 552 CCAGG CC^[AT]GG
MvnI 346 CGCG CG^CG
NaeI 233 GCCGGC GCC^GGC
NgoMIV 233 GCCGGC G^CCGGC
NlaIII 17 CATG CATG^
NspV 578 TTCGAA TT^CGAA
PpuMI 116 GGGACCT [GA]G^G[AT]CC[CT]
PstI 374 CTGCAG CTGCA^G
PvuII 377 CAGCTG CAG^CTG
RsaI 402 GTAC GT^AC
RsrII 322 CGGACCG CG^G[AT]CCG
SapI 174 GCTCTTC GCTCTTC(1/4)
ScaI 401 AGTACT AGT^ACT
SfcI 374 CTGCAG C^T[GA][CT]AG
SfuI 578 TTCGAA TT^CGAA
SmaI 450 CCCGGG CCC^GGG
TaqI 579 TCGA T^CGA
TfiI 576 GATTC G^A[AT]TC
Tsp45I 310 GTGAC ^GT[GC]AC
TthHB8I 579 TCGA T^CGA
XmaCI 450 CCCGGG C^CCGGG
XmaI 450 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 278 AGCT AG^CT
378 AGCT
AvaI 131 CCCGAG C^[CT]CG[GA]G
450 CCCGGG
AvaII 117 GGACC G^G[AT]CC
323 GGACC
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
436 GCTGAGG
BglI 557 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
614 GCCGGACTGGC
BsgI 254 CTGCAC GTGCAG(16/14)
600 CTGCAC
BsoBI 131 CCCGAG C^[CT]CG[GA]G
450 CCCGGG
BspMI 211 GCAGGT ACCTGC(4/8)
457 GCAGGT
EaeI 221 CGGCCA [CT]^GGCC[GA]
621 TGGCCG
FauI 416 CCCGC CCCGC(4/6)
531 GCGGG
SfaNI 292 GATGC GCATC(5/9)
547 GATGC
Tsp509I 63 AATT ^AATT
582 AATT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BanI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI Cac8I Cfr13I
DdeI DraII EcoO109I Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MnlI MspA1I MspI MwoI NciI
NlaIV PleI Sau96I ScrFI TseI
TspRI