Restriction map for
pF1KB9794
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 67 GGCGCC G[GA]^CG[CT]C
AlwI 401 GGATC GGATC(4/5)
Aor51HI 27 AGCGCT AGC^GCT
AscI 154 GGCGCGCC GG^CGCGCC
BalI 446 TGGCCA TGG^CCA
BanII 40 GGGCTC G[GA]GC[CT]^C
BbeI 67 GGCGCC GGCGC^C
BbsI 613 GAAGAC GAAGAC(2/6)
BbvCI 643 CCTCAGC CCTCAGC(-5/-2)
BcgI 527 CGATGAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 265 CTAG C^TAG
BglI 33 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 613 GAAGAC GAAGAC(2/6)
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BseRI 457 GAGGAG GAGGAG(10/8)
BsgI 520 CTGCAC GTGCAG(16/14)
BsiEI 405 CGACCG CG[GA][CT]^CG
BsrBI 78 GAGCGG CCGCTC(-3/-3)
BssHII 155 GCGCGC G^CGCGC
BstEII 465 GGTCACC G^GT[ACGT]ACC
BstPI 465 GGTCACC G^GT[ACGT]ACC
CpoI 607 CGGACCG CG^G[AT]CCG
DdeI 644 CTCAG C^T[ACGT]AG
DpnII 402 GATC ^GATC
DsaI 127 CCGTGG C^C[GA][CT]GG
EaeI 446 TGGCCA [CT]^GGCC[GA]
EarI 49 GAAGAG CTCTTC(1/4)
Eco47III 27 AGCGCT AGC^GCT
EcoO65I 465 GGTCACC G^GT[ACGT]ACC
EcoT14I 470 CCAAGG C^C[AT][AT]GG
HgaI 256 GACGC GACGC(5/10)
Hin1I 67 GGCGCC G[GA]^CG[CT]C
HinfI 325 GACTC G^A[ACGT]TC
HphI 467 TCACC GGTGA(8/7)
KasI 67 GGCGCC G^GCGCC
Ksp632I 49 GAAGAG CTCTTC(1/4)
MaeI 265 CTAG C^TAG
MaeIII 466 GTCAC ^GT[ACGT]AC
MboI 402 GATC ^GATC
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
NaeI 580 GCCGGC GCC^GGC
NarI 67 GGCGCC GG^CGCC
NdeII 402 GATC ^GATC
NgoMIV 580 GCCGGC G^CCGGC
PaeR7I 564 CTCGAG C^TCGAG
PleI 325 GACTC GAGTC(4/5)
PpuMI 269 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 607 CGGACCG CG^G[AT]CCG
Sau3AI 402 GATC ^GATC
SfcI 393 CTACAG C^T[GA][CT]AG
SfoI 67 GGCGCC GGC^GCC
SmaI 220 CCCGGG CCC^GGG
SmlI 564 CTCGAG C^T[CT][GA]AG
SphI 302 GCATGC GCATG^C
StyI 470 CCAAGG C^C[AT][AT]GG
Tsp45I 466 GTCAC ^GT[GC]AC
XhoI 564 CTCGAG C^TCGAG
XmaCI 220 CCCGGG C^CCGGG
XmaI 220 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 270 GGACC G^G[AT]CC
608 GGACC
BanI 67 GGCGCC G^G[CT][GA]CC
490 GGCACC
BpmI 75 CTGGAG CTGGAG(16/14)
412 CTGGAG
BsmFI 83 GGGAC GGGAC(10/14)
330 GTCCC
BsrFI 407 ACCGGC [GA]^CCGG[CT]
580 GCCGGC
Cfr10I 407 ACCGGC [GA]^CCGG[CT]
580 GCCGGC
DraII 248 AGGCCCT [GA]G^G[ACGT]CC[CT]
269 AGGACCT
EcoNI 419 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
494 CCTTCCCCAGG
EcoO109I 248 AGGCCCT [GA]G^G[ACGT]CC[CT]
269 AGGACCT
FauI 80 GCGGG CCCGC(4/6)
196 GCGGG
MaeII 335 ACGT A^CGT
533 ACGT
MboII 49 GAAGA GAAGA(8/7)
613 GAAGA
MspA1I 175 CCGCTG C[AC]G^C[GT]G
191 CCGCTG
NlaIII 303 CATG CATG^
312 CATG
NspI 302 GCATGC [GA]CATG^[CT]
311 ACATGC
StuI 416 AGGCCT AGG^CCT
640 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I