################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I CfoI Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I TspRI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 261 GCGG CCGC(-3/-1) AcsI 76 AAATTC [GA]^AATT[CT] AgeI 527 ACCGGT A^CCGGT AocI 25 CCTAAGG CC^T[ACGT]AGG ApoI 76 AAATTC [GA]^AATT[CT] Asp700I 128 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 264 GGATCC G^GATCC BlpI 325 GCTCAGC GC^T[ACGT]AGC BpmI 409 CTGGAG CTGGAG(16/14) Bpu1102I 325 GCTCAGC GC^T[ACGT]AGC BsaWI 527 ACCGGT [AT]^CCGG[AT] BsiYI 207 CCGATACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 207 CCGATACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 37 GTCTC GTCTC(1/5) BspMI 363 GCAGGT ACCTGC(4/8) BsrFI 527 ACCGGT [GA]^CCGG[CT] BsrI 350 ACTGG ACTGG(1/-1) BstF5I 293 CATCC GGATG(2/0) Bsu36I 25 CCTAAGG CC^T[ACGT]AGG CelII 325 GCTCAGC GC^T[ACGT]AGC Cfr10I 527 ACCGGT [GA]^CCGG[CT] DraI 112 TTTAAA TTT^AAA Eco81I 25 CCTAAGG CC^T[ACGT]AGG EcoT14I 371 CCAAGG C^C[AT][AT]GG FauI 261 GCGGG CCCGC(4/6) FokI 293 CATCC GGATG(9/13) HapII 528 CCGG C^CGG HincII 393 GTCAAC GT[CT]^[GA]AC HindII 393 GTCAAC GT[CT]^[GA]AC HinfI 545 GAGTC G^A[ACGT]TC HpaII 528 CCGG C^CGG MseI 113 TTAA T^TAA MslI 418 CACCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 328 CAGCTG C[AC]G^C[GT]G MspI 528 CCGG C^CGG NlaIII 163 CATG CATG^ PinAI 527 ACCGGT A^CCGGT PleI 545 GAGTC GAGTC(4/5) PvuII 328 CAGCTG CAG^CTG ScaI 455 AGTACT AGT^ACT StyI 371 CCAAGG C^C[AT][AT]GG Tru9I 113 TTAA T^TAA XbaI 124 TCTAGA T^CTAGA XmnI 128 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 456 GTAC GT^AC 507 GTAC BalI 188 TGGCCA TGG^CCA 368 TGGCCA BbvI 87 GCAGC GCAGC(8/12) 90 GCAGC BfaI 125 CTAG C^TAG 521 CTAG BsgI 361 GTGCAG GTGCAG(16/14) 531 GTGCAG BstYI 148 AGATCC [GA]^GATC[CT] 264 GGATCC DpnII 149 GATC ^GATC 265 GATC EaeI 188 TGGCCA [CT]^GGCC[GA] 368 TGGCCA Fnu4HI 87 GCAGC GC^[ACGT]GC 90 GCAGC ItaI 87 GCAGC GC^[ACGT]GC 90 GCAGC MaeI 125 CTAG C^TAG 521 CTAG MaeII 47 ACGT A^CGT 104 ACGT MboI 149 GATC ^GATC 265 GATC MflI 148 AGATCC [GA]^GATC[CT] 264 GGATCC MluNI 188 TGGCCA TGG^CCA 368 TGGCCA MscI 188 TGGCCA TGG^CCA 368 TGGCCA NdeII 149 GATC ^GATC 265 GATC NlaIV 264 GGATCC GG[ACGT]^[ACGT]CC 273 GGAACC RsaI 456 GTAC GT^AC 507 GTAC Sau3AI 149 GATC ^GATC 265 GATC TaqI 197 TCGA T^CGA 461 TCGA TseI 87 GCAGC G^C[AT]GC 90 GCAGC TthHB8I 197 TCGA T^CGA 461 TCGA XhoII 148 AGATCC [GA]^GATC[CT] 264 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI AlwI BsaJI BssKI BstNI DdeI EarI EcoRII HaeIII Ksp632I MboII MnlI MvaI ScrFI Tsp509I