################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I MaeI MaeII MamI MboII MfeI MflI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 33 GTAC GT^AC AflIII 29 ACGCGT A^C[GA][CT]GT AspHI 7 GAGCTC G[AT]GC[AT]^C AvaI 132 CCCGGG C^[CT]CG[GA]G AviII 404 TGCGCA TGC^GCA BlpI 306 GCTCAGC GC^T[ACGT]AGC Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC BsgI 158 CTGCAC GTGCAG(16/14) BsiEI 280 CGGCCG CG[GA][CT]^CG BsiHKAI 7 GAGCTC G[AT]GC[AT]^C BsoBI 132 CCCGGG C^[CT]CG[GA]G BssHII 319 GCGCGC G^CGCGC BssSI 664 CACGAG CACGAG(-5/-1) BstF5I 378 CATCC GGATG(2/0) CelII 306 GCTCAGC GC^T[ACGT]AGC DpnII 420 GATC ^GATC DraIII 567 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 280 CGGCCG C^GGCCG EclXI 280 CGGCCG C^GGCCG Eco52I 280 CGGCCG C^GGCCG FokI 378 CATCC GGATG(9/13) FspI 404 TGCGCA TGC^GCA MaeIII 662 GTCAC ^GT[ACGT]AC MboI 420 GATC ^GATC MluI 29 ACGCGT A^CGCGT NdeII 420 GATC ^GATC NotI 279 GCGGCCGC GC^GGCCGC NspI 335 GCATGC [GA]CATG^[CT] PflMI 513 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 33 GTAC GT^AC SacI 7 GAGCTC GAGCT^C Sau3AI 420 GATC ^GATC SmaI 132 CCCGGG CCC^GGG SmlI 10 CTCAAG C^T[CT][GA]AG SphI 335 GCATGC GCATG^C TaqI 227 TCGA T^CGA Tsp45I 662 GTCAC ^GT[GC]AC TthHB8I 227 TCGA T^CGA Van91I 513 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 132 CCCGGG C^CCGGG XmaI 132 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwNI 466 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 514 CAGGGCCTG ApaI 129 GGGCCC GGGCC^C 601 GGGCCC BglI 79 GCCTACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 131 GCCCGGGCGGC BsaI 73 GGTCTC GGTCTC(1/5) 296 GGTCTC BsmAI 74 GTCTC GTCTC(1/5) 297 GTCTC BsrBI 204 GAGCGG CCGCTC(-3/-3) 325 GAGCGG EcoNI 453 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 501 CCTCAACCAGG KspI 111 CCGCGG CCGC^GG 149 CCGCGG NlaIII 336 CATG CATG^ 456 CATG SacII 111 CCGCGG CCGC^GG 149 CCGCGG SfaNI 480 GATGC GCATC(5/9) 311 GCATC TspRI 567 CACTG CA[GC]TG[ACGT][ACGT]^ 658 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AcyI AluI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI TseI