Restriction map for
pF1KB9806
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BfaI BglII BlnI BpmI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FokI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfaNI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflII 193 CTTAAG C^TTAAG
ApaI 118 GGGCCC GGGCC^C
BbsI 459 GAAGAC GAAGAC(2/6)
BclI 137 TGATCA T^GATCA
BfrI 193 CTTAAG C^TTAAG
BpuAI 459 GAAGAC GAAGAC(2/6)
BsgI 289 GTGCAG GTGCAG(16/14)
BsiEI 209 CGGCCG CG[GA][CT]^CG
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BsrDI 170 GCAATG GCAATG(2/0)
BstNI 285 CCTGG CC^[AT]GG
BstXI 143 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 138 GATC ^GATC
DraI 322 TTTAAA TTT^AAA
EaeI 209 CGGCCG [CT]^GGCC[GA]
EagI 209 CGGCCG C^GGCCG
EarI 513 GAAGAG CTCTTC(1/4)
EclXI 209 CGGCCG C^GGCCG
Eco52I 209 CGGCCG C^GGCCG
EcoNI 192 CCTTAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 285 CCTGG ^CC[AT]GG
FbaI 137 TGATCA T^GATCA
HgaI 497 GCGTC GACGC(5/10)
Ksp632I 513 GAAGAG CTCTTC(1/4)
MaeII 224 ACGT A^CGT
MboI 138 GATC ^GATC
MslI 282 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 285 CCTGG CC^[AT]GG
NaeI 91 GCCGGC GCC^GGC
NdeII 138 GATC ^GATC
NgoMIV 91 GCCGGC G^CCGGC
PstI 434 CTGCAG CTGCA^G
SapI 513 GAAGAGC GCTCTTC(1/4)
Sau3AI 138 GATC ^GATC
SfcI 434 CTGCAG C^T[GA][CT]AG
SfiI 201 GGCCTTAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 38 CGCCGGTG C[GA]^CCGG[CT]G
StuI 200 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 402 CGCG CG^CG
486 CGCG
BanII 118 GGGCCC G[GA]GC[CT]^C
516 GAGCCC
BbeI 36 GGCGCC GGCGC^C
379 GGCGCC
BlpI 100 GCTAAGC GC^T[ACGT]AGC
616 GCTAAGC
Bpu1102I 100 GCTAAGC GC^T[ACGT]AGC
616 GCTAAGC
BsmAI 22 GAGAC GTCTC(1/5)
127 GTCTC
BsrI 304 ACTGG ACTGG(1/-1)
124 CCAGT
BstUI 402 CGCG CG^CG
486 CGCG
CelII 100 GCTAAGC GC^T[ACGT]AGC
616 GCTAAGC
DraII 117 GGGGCCC [GA]G^G[ACGT]CC[CT]
118 GGGCCCC
DsaI 401 CCGCGG C^C[GA][CT]GG
485 CCGCGG
EcoO109I 117 GGGGCCC [GA]G^G[ACGT]CC[CT]
118 GGGCCCC
HinfI 462 GACTC G^A[ACGT]TC
564 GAGTC
HphI 270 GGTGA GGTGA(8/7)
140 TCACC
KasI 36 GGCGCC G^GCGCC
379 GGCGCC
KspI 401 CCGCGG CCGC^GG
485 CCGCGG
MnlI 258 CCTC CCTC(7/6)
344 CCTC
MvnI 402 CGCG CG^CG
486 CGCG
NarI 36 GGCGCC GG^CGCC
379 GGCGCC
PleI 462 GACTC GAGTC(4/5)
564 GAGTC
SacII 401 CCGCGG CCGC^GG
485 CCGCGG
SfoI 36 GGCGCC GGC^GCC
379 GGCGCC
SmlI 193 CTTAAG C^T[CT][GA]AG
259 CTCAAG
TspRI 303 CACTG CA[GC]TG[ACGT][ACGT]^
452 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcyI AluI BanI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BsrFI BssKI
Cac8I CfoI Cfr10I Cfr13I DdeI
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI MboII MseI MspA1I MspI
MwoI NciI NlaIV Sau96I ScrFI
StyI Tru9I TseI