Restriction map for
pF1KB9812
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraIII
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 728 ACACGT A^C[GA][CT]GT
AhdI 509 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 226 GGGCCC GGGCC^C
AspEI 509 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 206 CCCGAG C^[CT]CG[GA]G
AvaII 309 GGTCC G^G[AT]CC
BbsI 82 GAAGAC GAAGAC(2/6)
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BciVI 716 GGATAC GTATCC(6/5)
BlpI 315 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 315 GCTGAGC GC^T[ACGT]AGC
BpuAI 82 GAAGAC GAAGAC(2/6)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsmAI 288 GAGAC GTCTC(1/5)
BsmBI 288 GAGACG CGTCTC(1/5)
BsmFI 507 GGGAC GGGAC(10/14)
BsmI 241 GCATTC GAATGC(1/-1)
BsoBI 206 CCCGAG C^[CT]CG[GA]G
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BsrDI 259 GCAATG GCAATG(2/0)
BsrFI 597 GCCGGT [GA]^CCGG[CT]
BssSI 700 CTCGTG CACGAG(-5/-1)
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstXI 146 CCAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 315 GCTGAGC GC^T[ACGT]AGC
Cfr10I 597 GCCGGT [GA]^CCGG[CT]
DrdI 477 GACCGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 491 CGGCCA [CT]^GGCC[GA]
Eam1105I 509 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
HgaI 290 GACGC GACGC(5/10)
KspI 304 CCGCGG CCGC^GG
MspA1I 304 CCGCGG C[AC]G^C[GT]G
PpuMI 308 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 304 CCGCGG CCGC^GG
ScaI 443 AGTACT AGT^ACT
SexAI 512 ACCAGGT A^CC[AT]GGT
SfaNI 44 GCATC GCATC(5/9)
SspI 563 AATATT AAT^ATT
XcmI 146 CCAAGCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 414 GTAC GT^AC
444 GTAC
AlwI 114 GATCC GGATC(4/5)
63 GGATC
AspHI 180 GAGCAC G[AT]GC[AT]^C
674 GAGCAC
BanII 226 GGGCCC G[GA]GC[CT]^C
337 GAGCCC
BbvI 374 GCAGC GCAGC(8/12)
276 GCTGC
BsiHKAI 180 GAGCAC G[AT]GC[AT]^C
674 GAGCAC
BsrI 635 ACTGG ACTGG(1/-1)
250 CCAGT
BstF5I 45 CATCC GGATG(2/0)
656 CATCC
Cac8I 228 GCCCGC GC[ACGT]^[ACGT]GC
707 GCTGGC
DraII 225 AGGGCCC [GA]G^G[ACGT]CC[CT]
308 GGGTCCT
EcoO109I 225 AGGGCCC [GA]G^G[ACGT]CC[CT]
308 GGGTCCT
EcoT14I 48 CCAAGG C^C[AT][AT]GG
332 CCTTGG
Fnu4HI 276 GCTGC GC^[ACGT]GC
374 GCAGC
FokI 45 CATCC GGATG(9/13)
656 CATCC
HapII 214 CCGG C^CGG
598 CCGG
HpaII 214 CCGG C^CGG
598 CCGG
ItaI 276 GCTGC GC^[ACGT]GC
374 GCAGC
MboII 82 GAAGA GAAGA(8/7)
764 GAAGA
MseI 107 TTAA T^TAA
164 TTAA
MspI 214 CCGG C^CGG
598 CCGG
NlaIII 60 CATG CATG^
357 CATG
RsaI 414 GTAC GT^AC
444 GTAC
StyI 48 CCAAGG C^C[AT][AT]GG
332 CCTTGG
TaqI 497 TCGA T^CGA
751 TCGA
Tru9I 107 TTAA T^TAA
164 TTAA
TseI 276 GCTGC G^C[AT]GC
374 GCAGC
Tsp509I 94 AATT ^AATT
172 AATT
TthHB8I 497 TCGA T^CGA
751 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AluI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI Cfr13I DdeI DpnII DsaI
EcoRII FauI HaeIII HinfI HphI
MaeII MaeIII MboI MnlI MvaI
MvnI MwoI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI Tsp45I TspRI