##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsiEI       BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HindIII     HinfI       HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MboI        MfeI        MflI        MluI        MslI        
MunI        NarI        NcoI        NdeI        NdeII       
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          127  GTCTAC               GT^[AC][GT]AC
AccIII        350  TCCGGA               T^CCGGA
AfaI          747  GTAC                 GT^AC
AgeI          826  ACCGGT               A^CCGGT
BanI          793  GGTGCC               G^G[CT][GA]CC
BsaAI         604  TACGTG               [CT]AC^GT[GA]
BseAI         350  TCCGGA               T^CCGGA
BseRI         688  CTCCTC               GAGGAG(10/8)
BsgI          302  GTGCAG               GTGCAG(16/14)
BsmBI         109  CGTCTC               CGTCTC(1/5)
BspEI         350  TCCGGA               T^CCGGA
BsrI          624  ACTGG                ACTGG(1/-1)
BstEII        822  GGTGACC              G^GT[ACGT]ACC
BstF5I         78  CATCC                GGATG(2/0)
BstPI         822  GGTGACC              G^GT[ACGT]ACC
BstXI         439  CCAGGAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          298  CTGAG                C^T[ACGT]AG
EcoNI         347  CCTTCCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       822  GGTGACC              G^GT[ACGT]ACC
FokI           78  CATCC                GGATG(9/13)
HaeII         195  GGCGCT               [GA]GCGC^[CT]
HgaI          642  GACGC                GACGC(5/10)
HincII        490  GTCAAC               GT[CT]^[GA]AC
HindII        490  GTCAAC               GT[CT]^[GA]AC
MroI          350  TCCGGA               T^CCGGA
MseI          812  TTAA                 T^TAA
MspA1I        817  CCGCTG               C[AC]G^C[GT]G
NheI          198  GCTAGC               G^CTAGC
PinAI         826  ACCGGT               A^CCGGT
RsaI          747  GTAC                 GT^AC
SfaNI         476  GCATC                GCATC(5/9)
StuI          515  AGGCCT               AGG^CCT
Tru9I         812  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

ApaI          392  GGGCCC               GGGCC^C
              683  GGGCCC              
BbsI          855  GAAGAC               GAAGAC(2/6)
              241  GTCTTC              
BpuAI         855  GAAGAC               GAAGAC(2/6)
              241  GTCTTC              
BsmAI         110  GTCTC                GTCTC(1/5)
              169  GTCTC               
BsmFI         521  GGGAC                GGGAC(10/14)
              891  GTCCC               
BspMI           5  ACCTGC               ACCTGC(4/8)
              155  ACCTGC              
BsrBI          61  CCGCTC               CCGCTC(-3/-3)
              630  CCGCTC              
EcoT14I       332  CCTTGG               C^C[AT][AT]GG
              581  CCTTGG              
SfiI          384  GGCCATCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              675  GGCCGGCGGGGCC       
StyI          332  CCTTGG               C^C[AT][AT]GG
              581  CCTTGG              
Tsp45I        407  GTCAC                ^GT[GC]AC
              823  GTGAC               
TspRI         623  CACTG                CA[GC]TG[ACGT][ACGT]^
              860  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BalI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BsaWI       BsiYI       
BslI        Bsp1286I    BsrFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboII       MluNI       MnlI        MscI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NgoMIV      NlaIV       PpuMI       Sau96I      
ScrFI       TseI