Restriction map for
pF1KB9817
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AlwI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspI AvaI AviII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MboI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 824 ACGTGT A^C[GA][CT]GT
AhdI 498 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 686 GTGCAC G^TGCAC
AlwNI 871 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 686 GTGCAC G^TGCAC
AseI 454 ATTAAT AT^TAAT
AsnI 454 ATTAAT AT^TAAT
AspEI 498 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 686 GTGCAC G[AT]GC[AT]^C
AvaII 101 GGTCC G^G[AT]CC
AvrII 510 CCTAGG C^CTAGG
BbsI 660 GTCTTC GAAGAC(2/6)
BlnI 510 CCTAGG C^CTAGG
BmyI 686 GTGCAC G[AGT]GC[ACT]^C
BpmI 85 CTGGAG CTGGAG(16/14)
BpuAI 660 GTCTTC GAAGAC(2/6)
BsaAI 548 TACGTG [CT]AC^GT[GA]
BsiHKAI 686 GTGCAC G[AT]GC[AT]^C
BsmAI 506 GTCTC GTCTC(1/5)
BsmBI 505 CGTCTC CGTCTC(1/5)
Bsp1286I 686 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 325 TGTACA T^GTACA
BspMI 338 GCAGGT ACCTGC(4/8)
BsrGI 325 TGTACA T^GTACA
BstAPI 722 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 371 TTCGAA TT^CGAA
CfoI 731 GCGC GCG^C
DraI 130 TTTAAA TTT^AAA
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 498 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 24 GAAGAG CTCTTC(1/4)
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 281 GATATC GAT^ATC
EcoT14I 510 CCTAGG C^C[AT][AT]GG
HhaI 731 GCGC GCG^C
HinP1I 731 GCGC G^CGC
Ksp632I 24 GAAGAG CTCTTC(1/4)
MaeIII 698 GTGAC ^GT[ACGT]AC
MfeI 329 CAATTG C^AATTG
MunI 329 CAATTG C^AATTG
NlaIV 101 GGTCCC GG[ACGT]^[ACGT]CC
NspV 371 TTCGAA TT^CGAA
PshBI 454 ATTAAT AT^TAAT
PstI 868 CTGCAG CTGCA^G
SfcI 868 CTGCAG C^T[GA][CT]AG
SfuI 371 TTCGAA TT^CGAA
SspBI 325 TGTACA T^GTACA
SspI 244 AATATT AAT^ATT
StyI 510 CCTAGG C^C[AT][AT]GG
Tsp45I 698 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 417 GAATTT [GA]^AATT[CT]
604 GAATTT
ApoI 417 GAATTT [GA]^AATT[CT]
604 GAATTT
BbvI 90 GCAGC GCAGC(8/12)
870 GCAGC
BfaI 94 CTAG C^TAG
511 CTAG
BsmFI 228 GGGAC GGGAC(10/14)
102 GTCCC
BsmI 18 GAATGC GAATGC(1/-1)
727 GAATGC
BssKI 84 CCTGG ^CC[ACGT]GG
136 CCTGG
BstF5I 60 GGATG GGATG(2/0)
772 GGATG
BstNI 84 CCTGG CC^[AT]GG
136 CCTGG
EcoRII 84 CCTGG ^CC[AT]GG
136 CCTGG
Fnu4HI 90 GCAGC GC^[ACGT]GC
870 GCAGC
FokI 60 GGATG GGATG(9/13)
772 GGATG
HaeIII 81 GGCC GG^CC
749 GGCC
HinfI 197 GATTC G^A[ACGT]TC
887 GAATC
HphI 165 GGTGA GGTGA(8/7)
200 TCACC
ItaI 90 GCAGC GC^[ACGT]GC
870 GCAGC
MaeI 94 CTAG C^TAG
511 CTAG
MvaI 84 CCTGG CC^[AT]GG
136 CCTGG
ScrFI 84 CCTGG CC^[ACGT]GG
136 CCTGG
TaqI 372 TCGA T^CGA
890 TCGA
TfiI 197 GATTC G^A[AT]TC
887 GAATC
TseI 90 GCAGC G^C[AT]GC
870 GCAGC
TthHB8I 372 TCGA T^CGA
890 TCGA
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsrI Cac8I Cfr13I DdeI MaeII
MboII MnlI MseI MwoI NlaIII
RsaI Sau96I SfaNI Tru9I Tsp509I
TspRI