Restriction map for
pF1KB9818
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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ApaI 806 GGGCCC GGGCC^C
AspHI 682 GAGCAC G[AT]GC[AT]^C
AvrII 522 CCTAGG C^CTAGG
BciVI 275 GTATCC GTATCC(6/5)
BclI 848 TGATCA T^GATCA
BfaI 523 CTAG C^TAG
BglI 43 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 425 AGATCT A^GATCT
BlnI 522 CCTAGG C^CTAGG
BsaI 526 GGTCTC GGTCTC(1/5)
BsiHKAI 682 GAGCAC G[AT]GC[AT]^C
BspHI 599 TCATGA T^CATGA
BsrDI 607 GCAATG GCAATG(2/0)
BstF5I 884 CATCC GGATG(2/0)
EcoNI 126 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 775 CCCGC CCCGC(4/6)
FbaI 848 TGATCA T^GATCA
FokI 884 CATCC GGATG(9/13)
HincII 150 GTCAAC GT[CT]^[GA]AC
HindII 150 GTCAAC GT[CT]^[GA]AC
MaeI 523 CTAG C^TAG
MaeII 473 ACGT A^CGT
MspA1I 454 CAGCTG C[AC]G^C[GT]G
PflMI 720 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 454 CAGCTG CAG^CTG
RcaI 599 TCATGA T^CATGA
Van91I 720 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 741 GTAC GT^AC
750 GTAC
AlwI 157 GATCC GGATC(4/5)
772 GATCC
AvaII 114 GGTCC G^G[AT]CC
799 GGTCC
BanII 700 GAGCCC G[GA]GC[CT]^C
806 GGGCCC
BsmAI 3 GTCTC GTCTC(1/5)
527 GTCTC
BspMI 517 ACCTGC ACCTGC(4/8)
756 GCAGGT
BsrFI 493 ACCGGC [GA]^CCGG[CT]
752 ACCGGC
BsrI 227 CCAGT ACTGG(1/-1)
364 CCAGT
BstYI 156 AGATCC [GA]^GATC[CT]
425 AGATCT
Cfr10I 493 ACCGGC [GA]^CCGG[CT]
752 ACCGGC
EaeI 495 CGGCCA [CT]^GGCC[GA]
618 CGGCCA
EarI 250 GAAGAG CTCTTC(1/4)
916 GAAGAG
EcoT14I 522 CCTAGG C^C[AT][AT]GG
802 CCAAGG
Ksp632I 250 GAAGAG CTCTTC(1/4)
916 GAAGAG
MaeIII 367 GTCAC ^GT[ACGT]AC
514 GTCAC
MflI 156 AGATCC [GA]^GATC[CT]
425 AGATCT
MslI 468 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
499 CACTCCTATG
RsaI 741 GTAC GT^AC
750 GTAC
StyI 522 CCTAGG C^C[AT][AT]GG
802 CCAAGG
TaqI 213 TCGA T^CGA
351 TCGA
Tsp45I 367 GTCAC ^GT[GC]AC
514 GTCAC
Tsp509I 403 AATT ^AATT
588 AATT
TthHB8I 213 TCGA T^CGA
351 TCGA
XhoII 156 AGATCC [GA]^GATC[CT]
425 AGATCT
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AocI BanI BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BssKI BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII Eco81I EcoO109I EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SmlI
TseI TspRI