Restriction map for
pF1KB9825
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AvaI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MamI MluI
MluNI MroI MscI MseI MspA1I
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 783 GGTACC G^GTACC
AccII 803 CGCG CG^CG
AciI 366 CCGC CCGC(-3/-1)
AhdI 301 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 434 AGCGCT AGC^GCT
Asp718I 783 GGTACC G^GTACC
AspEI 301 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 706 GAGCAC G[AT]GC[AT]^C
AviII 81 TGCGCA TGC^GCA
BbsI 3 GTCTTC GAAGAC(2/6)
BcnI 787 CCCGG CC^[GC]GG
BglII 584 AGATCT A^GATCT
BpuAI 3 GTCTTC GAAGAC(2/6)
BsgI 754 GTGCAG GTGCAG(16/14)
BsiHKAI 706 GAGCAC G[AT]GC[AT]^C
BsmAI 118 GTCTC GTCTC(1/5)
BsrFI 776 ACCGGC [GA]^CCGG[CT]
BssHII 802 GCGCGC G^CGCGC
BstUI 803 CGCG CG^CG
BstXI 380 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 584 AGATCT [GA]^GATC[CT]
Cfr10I 776 ACCGGC [GA]^CCGG[CT]
Eam1105I 301 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 434 AGCGCT AGC^GCT
EcoNI 34 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 81 TGCGCA TGC^GCA
HgaI 301 GACGC GACGC(5/10)
KpnI 783 GGTACC GGTAC^C
MaeII 497 ACGT A^CGT
MfeI 626 CAATTG C^AATTG
MflI 584 AGATCT [GA]^GATC[CT]
MslI 492 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 626 CAATTG C^AATTG
MvnI 803 CGCG CG^CG
NciI 787 CCCGG CC^[GC]GG
NlaIII 868 CATG CATG^
NspI 867 GCATGT [GA]CATG^[CT]
PpuMI 180 AGGTCCT [GA]G^G[AT]CC[CT]
StuI 600 AGGCCT AGG^CCT
TaqI 375 TCGA T^CGA
TthHB8I 375 TCGA T^CGA
XhoII 584 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 774 GTAC GT^AC
784 GTAC
AocI 34 CCTGAGG CC^T[ACGT]AGG
196 CCTCAGG
BanI 145 GGCACC G^G[CT][GA]CC
783 GGTACC
BanII 724 GAGCCC G[GA]GC[CT]^C
830 GGGCTC
BsmFI 139 GTCCC GGGAC(10/14)
471 GTCCC
BsrDI 897 CATTGC GCAATG(2/0)
631 GCAATG
BstF5I 908 CATCC GGATG(2/0)
342 GGATG
Bsu36I 34 CCTGAGG CC^T[ACGT]AGG
196 CCTCAGG
DraII 62 AGGCCCT [GA]G^G[ACGT]CC[CT]
180 AGGTCCT
Eco81I 34 CCTGAGG CC^T[ACGT]AGG
196 CCTCAGG
EcoO109I 62 AGGCCCT [GA]G^G[ACGT]CC[CT]
180 AGGTCCT
EcoT14I 380 CCAAGG C^C[AT][AT]GG
826 CCAAGG
FokI 908 CATCC GGATG(9/13)
342 GGATG
HaeII 434 AGCGCT [GA]GCGC^[CT]
646 AGCGCC
HapII 777 CCGG C^CGG
788 CCGG
HpaII 777 CCGG C^CGG
788 CCGG
HphI 539 TCACC GGTGA(8/7)
740 TCACC
MspI 777 CCGG C^CGG
788 CCGG
RsaI 774 GTAC GT^AC
784 GTAC
StyI 380 CCAAGG C^C[AT][AT]GG
826 CCAAGG
Tsp45I 391 GTCAC ^GT[GC]AC
538 GTCAC
Tsp509I 427 AATT ^AATT
627 AATT
TspRI 25 CACTG CA[GC]TG[ACGT][ACGT]^
711 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AvaII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BspMI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI Ksp632I MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI TseI