Restriction map for
pF1KB9829
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MflI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI SwaI
Tru9I Tsp45I Tsp509I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 614 GTCGAC GT^[AC][GT]AC
AflIII 277 ACACGT A^C[GA][CT]GT
AlwI 884 GATCC GGATC(4/5)
AocI 172 CCTGAGG CC^T[ACGT]AGG
AspI 865 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 148 CTCGAG C^[CT]CG[GA]G
AvaII 327 GGACC G^G[AT]CC
BalI 10 TGGCCA TGG^CCA
BbrPI 278 CACGTG CAC^GTG
BclI 44 TGATCA T^GATCA
BpmI 721 CTGGAG CTGGAG(16/14)
BsaAI 278 CACGTG [CT]AC^GT[GA]
BseRI 583 CTCCTC GAGGAG(10/8)
BsmI 675 GAATGC GAATGC(1/-1)
BsoBI 148 CTCGAG C^[CT]CG[GA]G
BspMI 333 GCAGGT ACCTGC(4/8)
BsrDI 665 CATTGC GCAATG(2/0)
BsrFI 329 ACCGGC [GA]^CCGG[CT]
BstAPI 660 GCAAGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 629 CCAAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 172 CCTGAGG CC^T[ACGT]AGG
Cfr10I 329 ACCGGC [GA]^CCGG[CT]
DsaI 50 CCATGG C^C[GA][CT]GG
EaeI 10 TGGCCA [CT]^GGCC[GA]
EarI 940 GAAGAG CTCTTC(1/4)
Eco81I 172 CCTGAGG CC^T[ACGT]AGG
EcoT14I 50 CCATGG C^C[AT][AT]GG
FbaI 44 TGATCA T^GATCA
HincII 614 GTCGAC GT[CT]^[GA]AC
HindII 614 GTCGAC GT[CT]^[GA]AC
HindIII 631 AAGCTT A^AGCTT
Ksp632I 940 GAAGAG CTCTTC(1/4)
MluNI 10 TGGCCA TGG^CCA
MscI 10 TGGCCA TGG^CCA
NcoI 50 CCATGG C^CATGG
PaeR7I 148 CTCGAG C^TCGAG
PflFI 865 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 767 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 581 GACTC GAGTC(4/5)
PmaCI 278 CACGTG CAC^GTG
PmlI 278 CACGTG CAC^GTG
SalI 614 GTCGAC G^TCGAC
SbfI 541 CCTGCAGG CCTGCA^GG
SmlI 148 CTCGAG C^T[CT][GA]AG
SphI 903 GCATGC GCATG^C
Sse8387I 541 CCTGCAGG CCTGCA^GG
StuI 517 AGGCCT AGG^CCT
StyI 50 CCATGG C^C[AT][AT]GG
TfiI 550 GAATC G^A[AT]TC
Tth111I 865 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 767 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 148 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 168 GGGCCC GGGCC^C
776 GGGCCC
BglI 3 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
422 GCCCAGCTGGC
BsrI 824 ACTGG ACTGG(1/-1)
139 CCAGT
DpnII 45 GATC ^GATC
884 GATC
DraIII 464 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
768 CACTTCGTG
FauI 193 GCGGG CCCGC(4/6)
907 GCGGG
HinfI 550 GAATC G^A[ACGT]TC
581 GACTC
MaeII 279 ACGT A^CGT
859 ACGT
MboI 45 GATC ^GATC
884 GATC
NdeII 45 GATC ^GATC
884 GATC
NspI 878 ACATGC [GA]CATG^[CT]
903 GCATGC
PstI 542 CTGCAG CTGCA^G
554 CTGCAG
Sau3AI 45 GATC ^GATC
884 GATC
SexAI 384 ACCTGGT A^CC[AT]GGT
866 ACCTGGT
SfaNI 340 GATGC GCATC(5/9)
559 GATGC
TaqI 149 TCGA T^CGA
615 TCGA
TthHB8I 149 TCGA T^CGA
615 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI BanI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PvuII
Sau96I ScrFI SfcI TseI TspRI