Restriction map for
pF1KB9832
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 67 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 186 AGCGCT AGC^GCT
AvaI 5 CTCGAG C^[CT]CG[GA]G
AvaII 814 GGTCC G^G[AT]CC
BanI 250 GGTGCC G^G[CT][GA]CC
BbvCI 295 GCTGAGG CCTCAGC(-5/-2)
BglI 88 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 745 GTGCAG GTGCAG(16/14)
BsmAI 166 GAGAC GTCTC(1/5)
BsmFI 883 GTCCC GGGAC(10/14)
BsoBI 5 CTCGAG C^[CT]CG[GA]G
BspHI 728 TCATGA T^CATGA
BstAPI 345 GCATTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 734 CCAAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 187 GCGC GCG^C
Cfr13I 814 GGTCC G^G[ACGT]CC
DraI 318 TTTAAA TTT^AAA
DsaI 977 CCATGG C^C[GA][CT]GG
Eco47III 186 AGCGCT AGC^GCT
EcoRI 802 GAATTC G^AATTC
EcoT14I 977 CCATGG C^C[AT][AT]GG
HaeII 186 AGCGCT [GA]GCGC^[CT]
HhaI 187 GCGC GCG^C
HinP1I 187 GCGC G^CGC
MseI 319 TTAA T^TAA
NcoI 977 CCATGG C^CATGG
PaeR7I 5 CTCGAG C^TCGAG
PleI 106 GAGTC GAGTC(4/5)
PstI 953 CTGCAG CTGCA^G
RcaI 728 TCATGA T^CATGA
SacI 821 GAGCTC GAGCT^C
Sau96I 814 GGTCC G^G[ACGT]CC
SfaNI 176 GCATC GCATC(5/9)
SfcI 953 CTGCAG C^T[GA][CT]AG
StyI 977 CCATGG C^C[AT][AT]GG
TaqI 6 TCGA T^CGA
TfiI 63 GAATC G^A[AT]TC
Tru9I 319 TTAA T^TAA
TthHB8I 6 TCGA T^CGA
XhoI 5 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 756 GTAC GT^AC
765 GTAC
AspHI 460 GAGCAC G[AT]GC[AT]^C
821 GAGCTC
BamHI 501 GGATCC G^GATCC
714 GGATCC
BpmI 760 CTGGAG CTGGAG(16/14)
853 CTGGAG
BsiHKAI 460 GAGCAC G[AT]GC[AT]^C
821 GAGCTC
DraIII 476 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
608 CACTGGGTG
HaeIII 87 GGCC GG^CC
592 GGCC
HinfI 63 GAATC G^A[ACGT]TC
106 GAGTC
MaeII 990 ACGT A^CGT
1003 ACGT
MslI 430 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
514 CACTACTATG
RsaI 756 GTAC GT^AC
765 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
BanII BbvI BfaI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI SmlI TseI Tsp45I Tsp509I
TspRI XhoII XmnI