Restriction map for
pF1KB9840
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeII MfeI MluI MslI
MunI NcoI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 162 TCCGGA T^CCGGA
AocI 297 CCTGAGG CC^T[ACGT]AGG
ApaI 929 GGGCCC GGGCC^C
AseI 904 ATTAAT AT^TAAT
AsnI 904 ATTAAT AT^TAAT
BalI 704 TGGCCA TGG^CCA
BamHI 113 GGATCC G^GATCC
BanII 929 GGGCCC G[GA]GC[CT]^C
BclI 221 TGATCA T^GATCA
BfaI 655 CTAG C^TAG
BmyI 929 GGGCCC G[AGT]GC[ACT]^C
BsaBI 138 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 162 TCCGGA T^CCGGA
BsmBI 502 GAGACG CGTCTC(1/5)
Bsp1286I 929 GGGCCC G[AGT]GC[ACT]^C
BspEI 162 TCCGGA T^CCGGA
BspHI 533 TCATGA T^CATGA
BssHII 131 GCGCGC G^CGCGC
BstAPI 1055 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 291 GGTCACC G^GT[ACGT]ACC
BstPI 291 GGTCACC G^GT[ACGT]ACC
BstXI 947 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
DsaI 272 CCGCGG C^C[GA][CT]GG
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
EcoNI 965 CCTCTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 291 GGTCACC G^GT[ACGT]ACC
EcoRV 244 GATATC GAT^ATC
EcoT14I 891 CCAAGG C^C[AT][AT]GG
EcoT22I 824 ATGCAT ATGCA^T
FbaI 221 TGATCA T^GATCA
KspI 272 CCGCGG CCGC^GG
MaeI 655 CTAG C^TAG
MamI 138 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 704 TGGCCA TGG^CCA
MroI 162 TCCGGA T^CCGGA
MscI 704 TGGCCA TGG^CCA
MspA1I 272 CCGCGG C[AC]G^C[GT]G
NsiI 824 ATGCAT ATGCA^T
PflMI 633 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 728 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 904 ATTAAT AT^TAAT
RcaI 533 TCATGA T^CATGA
SacII 272 CCGCGG CCGC^GG
SexAI 716 ACCAGGT A^CC[AT]GGT
StyI 891 CCAAGG C^C[AT][AT]GG
Van91I 633 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 654 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 887 GGCGCC G[GA]^CG[CT]C
895 GGCGCC
BbeI 887 GGCGCC GGCGC^C
895 GGCGCC
BcnI 288 CCCGG CC^[GC]GG
926 CCGGG
BsaHI 887 GGCGCC G[GA]^CG[CT]C
895 GGCGCC
BsaWI 162 TCCGGA [AT]^CCGG[AT]
763 ACCGGA
BseRI 963 CTCCTC GAGGAG(10/8)
121 GAGGAG
BsgI 600 GTGCAG GTGCAG(16/14)
1062 GTGCAG
BsmFI 645 GGGAC GGGAC(10/14)
760 GGGAC
BspMI 387 GCAGGT ACCTGC(4/8)
975 GCAGGT
BsrBI 694 CCGCTC CCGCTC(-3/-3)
427 GAGCGG
BstF5I 3 GGATG GGATG(2/0)
822 GGATG
DdeI 298 CTGAG C^T[ACGT]AG
1046 CTGAG
DraII 283 AGGCCCC [GA]G^G[ACGT]CC[CT]
928 GGGGCCC
DraIII 318 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
465 CACCGAGTG
EcoO109I 283 AGGCCCC [GA]G^G[ACGT]CC[CT]
928 GGGGCCC
FokI 3 GGATG GGATG(9/13)
822 GGATG
HaeII 887 GGCGCC [GA]GCGC^[CT]
895 GGCGCC
HgaI 504 GACGC GACGC(5/10)
607 GACGC
Hin1I 887 GGCGCC G[GA]^CG[CT]C
895 GGCGCC
KasI 887 GGCGCC G^GCGCC
895 GGCGCC
MboII 90 GAAGA GAAGA(8/7)
241 GAAGA
NaeI 312 GCCGGC GCC^GGC
558 GCCGGC
NarI 887 GGCGCC GG^CGCC
895 GGCGCC
NciI 288 CCCGG CC^[GC]GG
926 CCGGG
NgoMIV 312 GCCGGC G^CCGGC
558 GCCGGC
SfoI 887 GGCGCC GGC^GCC
895 GGCGCC
XcmI 629 CCACCCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1001 CCAGTATTAGTCTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BbvI BpmI BsaJI
BsiYI BslI BsmAI BsrFI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII EaeI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MflI MnlI
MseI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII