Restriction map for
pF1KB9842
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiHKAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 487 AAATTC [GA]^AATT[CT]
AfaI 707 GTAC GT^AC
AlwNI 501 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 473 AGCGCT AGC^GCT
ApoI 487 AAATTC [GA]^AATT[CT]
BamHI 635 GGATCC G^GATCC
BanII 881 GGGCTC G[GA]GC[CT]^C
BcgI 866 GCAATCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 54 AGATCT A^GATCT
BlpI 180 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
BseRI 973 CTCCTC GAGGAG(10/8)
BsiWI 706 CGTACG C^GTACG
BspHI 827 TCATGA T^CATGA
BspMI 257 ACCTGC ACCTGC(4/8)
BsrBI 237 CCGCTC CCGCTC(-3/-3)
BssSI 202 CTCGTG CACGAG(-5/-1)
BstXI 645 CCAGTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 180 GCTGAGC GC^T[ACGT]AGC
DraII 832 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 966 CCATGG C^C[GA][CT]GG
EagI 234 CGGCCG C^GGCCG
EarI 1050 CTCTTC CTCTTC(1/4)
EclXI 234 CGGCCG C^GGCCG
Eco47III 473 AGCGCT AGC^GCT
Eco52I 234 CGGCCG C^GGCCG
EcoNI 351 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 832 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 750 GCGGG CCCGC(4/6)
HincII 382 GTCAAC GT[CT]^[GA]AC
HindII 382 GTCAAC GT[CT]^[GA]AC
Ksp632I 1050 CTCTTC CTCTTC(1/4)
MspA1I 918 CAGCTG C[AC]G^C[GT]G
NcoI 966 CCATGG C^CATGG
NdeI 492 CATATG CA^TATG
PpuMI 832 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 918 CAGCTG CAG^CTG
RcaI 827 TCATGA T^CATGA
RsaI 707 GTAC GT^AC
SapI 1049 GCTCTTC GCTCTTC(1/4)
SexAI 803 ACCTGGT A^CC[AT]GGT
SfcI 390 CTATAG C^T[GA][CT]AG
SmlI 976 CTCAAG C^T[CT][GA]AG
StuI 1033 AGGCCT AGG^CCT
TaqI 875 TCGA T^CGA
TthHB8I 875 TCGA T^CGA
XcmI 893 CCACCGAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 253 GTCTAC GT^[AC][GT]AC
559 GTCTAC
BalI 437 TGGCCA TGG^CCA
951 TGGCCA
BbvI 792 GCTGC GCAGC(8/12)
920 GCTGC
BclI 674 TGATCA T^GATCA
1001 TGATCA
BcnI 741 CCGGG CC^[GC]GG
879 CCGGG
BmyI 881 GGGCTC G[AGT]GC[ACT]^C
1060 GGGCAC
BsaI 850 GAGACC GGTCTC(1/5)
363 GGTCTC
BsiEI 234 CGGCCG CG[GA][CT]^CG
876 CGACCG
Bsp1286I 881 GGGCTC G[AGT]GC[ACT]^C
1060 GGGCAC
BstYI 54 AGATCT [GA]^GATC[CT]
635 GGATCC
CfoI 474 GCGC GCG^C
861 GCGC
FbaI 674 TGATCA T^GATCA
1001 TGATCA
HaeII 473 AGCGCT [GA]GCGC^[CT]
860 AGCGCC
HapII 741 CCGG C^CGG
879 CCGG
HhaI 474 GCGC GCG^C
861 GCGC
HinP1I 474 GCGC G^CGC
861 GCGC
HpaII 741 CCGG C^CGG
879 CCGG
MflI 54 AGATCT [GA]^GATC[CT]
635 GGATCC
MluNI 437 TGGCCA TGG^CCA
951 TGGCCA
MscI 437 TGGCCA TGG^CCA
951 TGGCCA
MseI 11 TTAA T^TAA
716 TTAA
MspI 741 CCGG C^CGG
879 CCGG
NciI 741 CCGGG CC^[GC]GG
879 CCGGG
NlaIV 635 GGATCC GG[ACGT]^[ACGT]CC
833 GGACCC
PleI 971 GACTC GAGTC(4/5)
1012 GACTC
Tru9I 11 TTAA T^TAA
716 TTAA
TseI 792 GCTGC G^C[AT]GC
920 GCTGC
Tsp45I 23 GTGAC ^GT[GC]AC
244 GTCAC
Tsp509I 64 AATT ^AATT
488 AATT
XhoII 54 AGATCT [GA]^GATC[CT]
635 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AvaII BfaI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI SfaNI
StyI TfiI TspRI