Restriction map for
pF1KB9845
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AlwNI AocI Aor51HI AscI
Asp700I AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaHI BsaI BseAI BsgI BsiWI
BspEI BspHI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 571 GGTACC G^GTACC
AccI 169 GTCTAC GT^[AC][GT]AC
AcsI 1044 AAATTT [GA]^AATT[CT]
AflIII 266 ACATGT A^C[GA][CT]GT
AgeI 443 ACCGGT A^CCGGT
AhdI 924 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 657 GTGCAC G^TGCAC
AlwI 350 GGATC GGATC(4/5)
ApaI 854 GGGCCC GGGCC^C
ApaLI 657 GTGCAC G^TGCAC
ApoI 1044 AAATTT [GA]^AATT[CT]
AseI 714 ATTAAT AT^TAAT
AsnI 714 ATTAAT AT^TAAT
Asp718I 571 GGTACC G^GTACC
AspEI 924 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 657 GTGCAC G[AT]GC[AT]^C
AvaI 1007 CCCGAG C^[CT]CG[GA]G
BanI 571 GGTACC G^G[CT][GA]CC
BanII 854 GGGCCC G[GA]GC[CT]^C
BglI 46 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 741 AGATCT A^GATCT
BpmI 246 CTGGAG CTGGAG(16/14)
BsaBI 504 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 443 ACCGGT [AT]^CCGG[AT]
BsiHKAI 657 GTGCAC G[AT]GC[AT]^C
BsmBI 105 CGTCTC CGTCTC(1/5)
BsmI 498 GAATGC GAATGC(1/-1)
BsoBI 1007 CCCGAG C^[CT]CG[GA]G
Bsp1407I 263 TGTACA T^GTACA
BspDI 771 ATCGAT AT^CGAT
BspLU11I 266 ACATGT A^CATGT
BsrBI 249 GAGCGG CCGCTC(-3/-3)
BsrDI 880 GCAATG GCAATG(2/0)
BsrGI 263 TGTACA T^GTACA
BstAPI 880 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 125 GCGC GCG^C
ClaI 771 ATCGAT AT^CGAT
CpoI 57 CGGTCCG CG^G[AT]CCG
DraII 853 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 665 CCGTGG C^C[GA][CT]GG
Eam1105I 924 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 559 CTCTTC CTCTTC(1/4)
EcoO109I 853 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 686 CCTTGG C^C[AT][AT]GG
EcoT22I 429 ATGCAT ATGCA^T
FauI 979 GCGGG CCCGC(4/6)
HgaI 40 GACGC GACGC(5/10)
HhaI 125 GCGC GCG^C
HinP1I 125 GCGC G^CGC
HindIII 1022 AAGCTT A^AGCTT
KpnI 571 GGTACC GGTAC^C
Ksp632I 559 CTCTTC CTCTTC(1/4)
MamI 504 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 715 TTAA T^TAA
MspA1I 450 CAGCGG C[AC]G^C[GT]G
NsiI 429 ATGCAT ATGCA^T
PinAI 443 ACCGGT A^CCGGT
PshBI 714 ATTAAT AT^TAAT
PstI 376 CTGCAG CTGCA^G
RsrII 57 CGGTCCG CG^G[AT]CCG
SexAI 779 ACCTGGT A^CC[AT]GGT
SspBI 263 TGTACA T^GTACA
SspI 1078 AATATT AAT^ATT
StyI 686 CCTTGG C^C[AT][AT]GG
TaqI 772 TCGA T^CGA
TfiI 696 GATTC G^A[AT]TC
Tru9I 715 TTAA T^TAA
Tsp45I 922 GTGAC ^GT[GC]AC
TthHB8I 772 TCGA T^CGA
XbaI 1027 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AluI 726 AGCT AG^CT
1023 AGCT
BalI 212 TGGCCA TGG^CCA
668 TGGCCA
BbsI 1063 GAAGAC GAAGAC(2/6)
229 GTCTTC
BbvI 410 GCAGC GCAGC(8/12)
43 GCTGC
BfaI 934 CTAG C^TAG
1028 CTAG
BpuAI 1063 GAAGAC GAAGAC(2/6)
229 GTCTTC
BsaAI 298 TACGTG [CT]AC^GT[GA]
912 CACGTA
BsiEI 86 CGGTCG CG[GA][CT]^CG
662 CGACCG
BsiYI 244 CCCTGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCCTGGGCCGG
BslI 244 CCCTGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCCTGGGCCGG
BspMI 158 ACCTGC ACCTGC(4/8)
629 ACCTGC
BsrI 347 ACTGG ACTGG(1/-1)
147 CCAGT
BstYI 350 GGATCT [GA]^GATC[CT]
741 AGATCT
HphI 921 GGTGA GGTGA(8/7)
599 TCACC
MaeI 934 CTAG C^TAG
1028 CTAG
MaeII 299 ACGT A^CGT
913 ACGT
MflI 350 GGATCT [GA]^GATC[CT]
741 AGATCT
MluNI 212 TGGCCA TGG^CCA
668 TGGCCA
MscI 212 TGGCCA TGG^CCA
668 TGGCCA
PleI 306 GACTC GAGTC(4/5)
1001 GACTC
SfcI 376 CTGCAG C^T[GA][CT]AG
648 CTACAG
SmlI 469 CTCAAG C^T[CT][GA]AG
849 CTTGAG
TseI 43 GCTGC G^C[AT]GC
410 GCAGC
TspRI 96 CACTG CA[GC]TG[ACGT][ACGT]^
435 CAGTG
XhoII 350 GGATCT [GA]^GATC[CT]
741 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AvaII BcnI BmyI
BsaJI BseRI BsmAI BsmFI Bsp1286I
BsrFI BssKI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI Tsp509I