Restriction map for
pF1KB9858
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsiEI BsiWI
BsmI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MboII MfeI MluI
MseI MunI MvnI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfuI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 132 GTAC GT^AC
AhdI 659 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 857 GGGCCC GGGCC^C
AspEI 659 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 830 GGCGCC GGCGC^C
BbvCI 185 CCTCAGC CCTCAGC(-5/-2)
BcnI 965 CCGGG CC^[GC]GG
BglI 787 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 867 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 867 GCTGAGC GC^T[ACGT]AGC
BsgI 70 GTGCAG GTGCAG(16/14)
BsmBI 1184 GAGACG CGTCTC(1/5)
Bsp1407I 131 TGTACA T^GTACA
BsrFI 833 GCCGGC [GA]^CCGG[CT]
BsrGI 131 TGTACA T^GTACA
CelII 867 GCTGAGC GC^T[ACGT]AGC
CfoI 831 GCGC GCG^C
Cfr10I 833 GCCGGC [GA]^CCGG[CT]
DraIII 382 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 659 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 425 CCTCCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 647 GCGGG CCCGC(4/6)
HaeII 830 GGCGCC [GA]GCGC^[CT]
HhaI 831 GCGC GCG^C
HinP1I 831 GCGC G^CGC
HinfI 574 GACTC G^A[ACGT]TC
HphI 1025 TCACC GGTGA(8/7)
KasI 830 GGCGCC G^GCGCC
MslI 297 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 645 CAGCGG C[AC]G^C[GT]G
NaeI 833 GCCGGC GCC^GGC
NarI 830 GGCGCC GG^CGCC
NciI 965 CCGGG CC^[GC]GG
NgoMIV 833 GCCGGC G^CCGGC
NheI 463 GCTAGC G^CTAGC
NspI 761 GCATGC [GA]CATG^[CT]
PleI 574 GACTC GAGTC(4/5)
PpuMI 923 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1043 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 132 GTAC GT^AC
SacI 120 GAGCTC GAGCT^C
SfoI 830 GGCGCC GGC^GCC
SgrAI 832 CGCCGGCG C[GA]^CCGG[CT]G
SphI 761 GCATGC GCATG^C
SspBI 131 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 85 TCCGGA T^CCGGA
734 TCCGGA
AlwNI 770 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1160 CAGCTCCTG
AocI 157 CCTAAGG CC^T[ACGT]AGG
928 CCTGAGG
AspHI 57 GAGCAC G[AT]GC[AT]^C
120 GAGCTC
BalI 2 TGGCCA TGG^CCA
1130 TGGCCA
BamHI 220 GGATCC G^GATCC
376 GGATCC
BsaWI 85 TCCGGA [AT]^CCGG[AT]
734 TCCGGA
BseAI 85 TCCGGA T^CCGGA
734 TCCGGA
BsiHKAI 57 GAGCAC G[AT]GC[AT]^C
120 GAGCTC
BspEI 85 TCCGGA T^CCGGA
734 TCCGGA
BstF5I 343 CATCC GGATG(2/0)
1140 GGATG
BstYI 220 GGATCC [GA]^GATC[CT]
376 GGATCC
Bsu36I 157 CCTAAGG CC^T[ACGT]AGG
928 CCTGAGG
DpnII 221 GATC ^GATC
377 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
1130 TGGCCA
Eco81I 157 CCTAAGG CC^T[ACGT]AGG
928 CCTGAGG
FokI 343 CATCC GGATG(9/13)
1140 GGATG
MboI 221 GATC ^GATC
377 GATC
MflI 220 GGATCC [GA]^GATC[CT]
376 GGATCC
MluNI 2 TGGCCA TGG^CCA
1130 TGGCCA
MroI 85 TCCGGA T^CCGGA
734 TCCGGA
MscI 2 TGGCCA TGG^CCA
1130 TGGCCA
NcoI 509 CCATGG C^CATGG
563 CCATGG
NdeII 221 GATC ^GATC
377 GATC
Sau3AI 221 GATC ^GATC
377 GATC
XhoII 220 GGATCC [GA]^GATC[CT]
376 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BssSI BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HgaI Hin1I HpaII ItaI MaeI
MaeIII MnlI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI StyI
TseI Tsp45I TspRI