Restriction map for
pF1KB9869
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BseAI BsiWI BsmBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1063 GACGTC GACGT^C
AccI 640 GTCTAC GT^[AC][GT]AC
AcsI 63 GAATTC [GA]^AATT[CT]
Alw44I 220 GTGCAC G^TGCAC
AlwI 1269 GATCC GGATC(4/5)
AocI 1072 CCTCAGG CC^T[ACGT]AGG
ApaI 801 GGGCCC GGGCC^C
ApaLI 220 GTGCAC G^TGCAC
ApoI 63 GAATTC [GA]^AATT[CT]
AspI 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 399 TGGCCA TGG^CCA
BbvCI 953 CCTCAGC CCTCAGC(-5/-2)
BlpI 100 GCTGAGC GC^T[ACGT]AGC
BpmI 1273 CTCCAG CTGGAG(16/14)
Bpu1102I 100 GCTGAGC GC^T[ACGT]AGC
BsaWI 132 TCCGGT [AT]^CCGG[AT]
BsgI 890 GTGCAG GTGCAG(16/14)
BsmAI 700 GAGAC GTCTC(1/5)
BspDI 1357 ATCGAT AT^CGAT
BssSI 598 CTCGTG CACGAG(-5/-1)
BstAPI 195 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1268 AGATCC [GA]^GATC[CT]
Bsu36I 1072 CCTCAGG CC^T[ACGT]AGG
CelII 100 GCTGAGC GC^T[ACGT]AGC
ClaI 1357 ATCGAT AT^CGAT
EarI 1183 GAAGAG CTCTTC(1/4)
Eco81I 1072 CCTCAGG CC^T[ACGT]AGG
EcoRI 63 GAATTC G^AATTC
HphI 1013 TCACC GGTGA(8/7)
Ksp632I 1183 GAAGAG CTCTTC(1/4)
MflI 1268 AGATCC [GA]^GATC[CT]
MluNI 399 TGGCCA TGG^CCA
MscI 399 TGGCCA TGG^CCA
MslI 1221 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 574 GCATGT [GA]CATG^[CT]
PflFI 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1249 GACTC GAGTC(4/5)
PpuMI 749 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1087 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 763 CTGCAG CTGCA^G
PvuII 229 CAGCTG CAG^CTG
SbfI 762 CCTGCAGG CCTGCA^GG
ScaI 296 AGTACT AGT^ACT
SmaI 8 CCCGGG CCC^GGG
SmlI 1292 CTCAAG C^T[CT][GA]AG
Sse8387I 762 CCTGCAGG CCTGCA^GG
Tth111I 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1268 AGATCC [GA]^GATC[CT]
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 246 CGCG CG^CG
717 CGCG
AcyI 84 GGCGTC G[GA]^CG[CT]C
1063 GACGTC
AspHI 220 GTGCAC G[AT]GC[AT]^C
730 GTGCTC
BsaHI 84 GGCGTC G[GA]^CG[CT]C
1063 GACGTC
BsiEI 308 CGGCCG CG[GA][CT]^CG
419 CGGCCG
BsiHKAI 220 GTGCAC G[AT]GC[AT]^C
730 GTGCTC
BsmI 457 GAATGC GAATGC(1/-1)
1045 GCATTC
BsrFI 191 GCCGGC [GA]^CCGG[CT]
310 GCCGGC
BstUI 246 CGCG CG^CG
717 CGCG
Cfr10I 191 GCCGGC [GA]^CCGG[CT]
310 GCCGGC
DpnII 922 GATC ^GATC
1269 GATC
EagI 308 CGGCCG C^GGCCG
419 CGGCCG
EclXI 308 CGGCCG C^GGCCG
419 CGGCCG
Eco52I 308 CGGCCG C^GGCCG
419 CGGCCG
HgaI 571 GACGC GACGC(5/10)
85 GCGTC
Hin1I 84 GGCGTC G[GA]^CG[CT]C
1063 GACGTC
MboI 922 GATC ^GATC
1269 GATC
MboII 1183 GAAGA GAAGA(8/7)
1266 GAAGA
MvnI 246 CGCG CG^CG
717 CGCG
NaeI 191 GCCGGC GCC^GGC
310 GCCGGC
NdeII 922 GATC ^GATC
1269 GATC
NgoMIV 191 GCCGGC G^CCGGC
310 GCCGGC
Sau3AI 922 GATC ^GATC
1269 GATC
SexAI 503 ACCTGGT A^CC[AT]GGT
560 ACCTGGT
SfaNI 835 GATGC GCATC(5/9)
1144 GATGC
SfcI 186 CTATAG C^T[GA][CT]AG
763 CTGCAG
SfiI 319 GGCCGCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
411 GGCCTTTTCGGCC
TfiI 177 GATTC G^A[AT]TC
1036 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeII
MaeIII MnlI MseI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI