Restriction map for
pF1KB9874
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SexAI SfaNI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 271 GTCTAC GT^[AC][GT]AC
AccII 1256 CGCG CG^CG
AlwI 381 GGATC GGATC(4/5)
AlwNI 1452 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 804 GAGCAC G[AT]GC[AT]^C
BalI 570 TGGCCA TGG^CCA
BbsI 255 GTCTTC GAAGAC(2/6)
BcnI 1302 CCGGG CC^[GC]GG
BpuAI 255 GTCTTC GAAGAC(2/6)
BsaAI 1486 CACGTA [CT]AC^GT[GA]
BsaBI 847 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 804 GAGCAC G[AT]GC[AT]^C
BsmAI 604 GTCTC GTCTC(1/5)
BsmI 98 GAATGC GAATGC(1/-1)
BspHI 512 TCATGA T^CATGA
BspMI 240 GCAGGT ACCTGC(4/8)
BsrBI 1161 CCGCTC CCGCTC(-3/-3)
BstBI 95 TTCGAA TT^CGAA
BstUI 1256 CGCG CG^CG
Cac8I 1233 GCCAGC GC[ACGT]^[ACGT]GC
DraIII 1486 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 595 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 570 TGGCCA [CT]^GGCC[GA]
EarI 313 GAAGAG CTCTTC(1/4)
EcoRI 760 GAATTC G^AATTC
FauI 1521 GCGGG CCCGC(4/6)
HapII 1302 CCGG C^CGG
HpaII 1302 CCGG C^CGG
Ksp632I 313 GAAGAG CTCTTC(1/4)
KspI 1255 CCGCGG CCGC^GG
MaeII 1487 ACGT A^CGT
MamI 847 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 570 TGGCCA TGG^CCA
MscI 570 TGGCCA TGG^CCA
MspI 1302 CCGG C^CGG
MvnI 1256 CGCG CG^CG
NciI 1302 CCGGG CC^[GC]GG
NcoI 567 CCATGG C^CATGG
NspV 95 TTCGAA TT^CGAA
PflMI 1095 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 830 GACTC GAGTC(4/5)
RcaI 512 TCATGA T^CATGA
SacII 1255 CCGCGG CCGC^GG
ScaI 1009 AGTACT AGT^ACT
SfuI 95 TTCGAA TT^CGAA
SpeI 1012 ACTAGT A^CTAGT
StuI 203 AGGCCT AGG^CCT
Van91I 1095 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1150 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvrII 376 CCTAGG C^CTAGG
696 CCTAGG
BbvI 118 GCAGC GCAGC(8/12)
179 GCTGC
BglII 68 AGATCT A^GATCT
1533 AGATCT
BlnI 376 CCTAGG C^CTAGG
696 CCTAGG
BmyI 804 GAGCAC G[AGT]GC[ACT]^C
1347 GGGCAC
BseRI 355 GAGGAG GAGGAG(10/8)
387 GAGGAG
BsmFI 5 GGGAC GGGAC(10/14)
1494 GTCCC
Bsp1286I 804 GAGCAC G[AGT]GC[ACT]^C
1347 GGGCAC
BstYI 68 AGATCT [GA]^GATC[CT]
1533 AGATCT
Cfr13I 279 GGGCC G^G[ACGT]CC
1332 GGGCC
DraI 703 TTTAAA TTT^AAA
897 TTTAAA
DsaI 567 CCATGG C^C[GA][CT]GG
1255 CCGCGG
EcoNI 1312 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1369 CCTTCACAAGG
EcoT22I 284 ATGCAT ATGCA^T
957 ATGCAT
MflI 68 AGATCT [GA]^GATC[CT]
1533 AGATCT
MslI 465 CACTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
879 CACTGCAGTG
NsiI 284 ATGCAT ATGCA^T
957 ATGCAT
PvuII 119 CAGCTG CAG^CTG
412 CAGCTG
Sau96I 279 GGGCC G^G[ACGT]CC
1332 GGGCC
SspI 627 AATATT AAT^ATT
1068 AATATT
TaqI 96 TCGA T^CGA
593 TCGA
TseI 118 GCAGC G^C[AT]GC
179 GCTGC
TthHB8I 96 TCGA T^CGA
593 TCGA
XhoII 68 AGATCT [GA]^GATC[CT]
1533 AGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BfaI BpmI BsaJI BsiYI BslI
BsrI BssKI BstEII BstF5I BstNI
BstPI DdeI DpnII EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI ScrFI SfcI SmlI
StyI TfiI Tru9I Tsp45I Tsp509I
TspRI