##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TaqI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       1290  GGTACC               G^GTACC
AciI         1322  CCGC                 CCGC(-3/-1)
AseI          424  ATTAAT               AT^TAAT
AsnI          424  ATTAAT               AT^TAAT
Asp718I      1290  GGTACC               G^GTACC
BanI         1290  GGTACC               G^G[CT][GA]CC
BbsI         1135  GAAGAC               GAAGAC(2/6)
BpuAI        1135  GAAGAC               GAAGAC(2/6)
BsaI          907  GAGACC               GGTCTC(1/5)
BseRI         209  CTCCTC               GAGGAG(10/8)
BsiYI         911  CCCCAGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          911  CCCCAGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI        1433  GCAATG               GCAATG(2/0)
BstXI         422  CCATTAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          279  CCATGG               C^C[GA][CT]GG
EcoRV        1276  GATATC               GAT^ATC
EcoT14I       279  CCATGG               C^C[AT][AT]GG
FauI         1321  CCCGC                CCCGC(4/6)
HapII         684  CCGG                 C^CGG
HgaI          473  GACGC                GACGC(5/10)
HpaII         684  CCGG                 C^CGG
HphI         1183  GGTGA                GGTGA(8/7)
KpnI         1290  GGTACC               GGTAC^C
MspA1I        734  CAGCTG               C[AC]G^C[GT]G
MspI          684  CCGG                 C^CGG
NcoI          279  CCATGG               C^CATGG
PshBI         424  ATTAAT               AT^TAAT
PstI            7  CTGCAG               CTGCA^G
PvuII         734  CAGCTG               CAG^CTG
SapI         1069  GAAGAGC              GCTCTTC(1/4)
SfaNI         264  GCATC                GCATC(5/9)
SfcI            7  CTGCAG               C^T[GA][CT]AG
StyI          279  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI        1309  GAGCTC               G[AT]GC[AT]^C
             1457  GAGCTC              
AvaII         920  GGACC                G^G[AT]CC
             1359  GGTCC               
BanII        1309  GAGCTC               G[GA]GC[CT]^C
             1457  GAGCTC              
BpmI          774  CTCCAG               CTGGAG(16/14)
              128  CTGGAG              
BsgI          369  CTGCAC               GTGCAG(16/14)
             1160  GTGCAG              
BsiHKAI      1309  GAGCTC               G[AT]GC[AT]^C
             1457  GAGCTC              
Bsp1407I      434  TGTACA               T^GTACA
              698  TGTACA              
BsrGI         434  TGTACA               T^GTACA
              698  TGTACA              
EarI          212  CTCTTC               CTCTTC(1/4)
             1069  GAAGAG              
HaeIII        107  GGCC                 GG^CC
              686  GGCC                
Ksp632I       212  CTCTTC               CTCTTC(1/4)
             1069  GAAGAG              
MaeIII         16  GTAAC                ^GT[ACGT]AC
              358  GTTAC               
SacI         1309  GAGCTC               GAGCT^C
             1457  GAGCTC              
SspBI         434  TGTACA               T^GTACA
              698  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BmyI        BsaJI       BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HinfI       ItaI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII