################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AflII AflIII AgeI AhdI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoO65I EcoRV EcoT22I FseI HgaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 106 TCCGGA T^CCGGA AclI 281 AACGTT AA^CGTT AcsI 1511 GAATTC [GA]^AATT[CT] AfaI 1825 GTAC GT^AC Alw44I 1561 GTGCAC G^TGCAC Aor51HI 863 AGCGCT AGC^GCT ApaLI 1561 GTGCAC G^TGCAC ApoI 1511 GAATTC [GA]^AATT[CT] AviII 1502 TGCGCA TGC^GCA BbrPI 1558 CACGTG CAC^GTG BbsI 576 GTCTTC GAAGAC(2/6) BcgI 1111 CGAAAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 815 GGATAC GTATCC(6/5) BclI 1815 TGATCA T^GATCA BglI 207 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 576 GTCTTC GAAGAC(2/6) BsaAI 1558 CACGTG [CT]AC^GT[GA] BsaI 506 GAGACC GGTCTC(1/5) BsaWI 106 TCCGGA [AT]^CCGG[AT] BseAI 106 TCCGGA T^CCGGA BsiEI 391 CGGCCG CG[GA][CT]^CG BsmBI 796 CGTCTC CGTCTC(1/5) BspEI 106 TCCGGA T^CCGGA BsrI 510 CCAGT ACTGG(1/-1) DrdI 533 GACATCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 391 CGGCCG [CT]^GGCC[GA] EagI 391 CGGCCG C^GGCCG EclXI 391 CGGCCG C^GGCCG Eco47III 863 AGCGCT AGC^GCT Eco52I 391 CGGCCG C^GGCCG EcoNI 1395 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1511 GAATTC G^AATTC FbaI 1815 TGATCA T^GATCA FspI 1502 TGCGCA TGC^GCA HphI 197 TCACC GGTGA(8/7) MroI 106 TCCGGA T^CCGGA MseI 1694 TTAA T^TAA MslI 1225 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 905 GCCGGC GCC^GGC NgoMIV 905 GCCGGC G^CCGGC NotI 390 GCGGCCGC GC^GGCCGC NspI 1230 ACATGC [GA]CATG^[CT] PflMI 678 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1558 CACGTG CAC^GTG PmlI 1558 CACGTG CAC^GTG Psp1406I 281 AACGTT AA^CGTT RsaI 1825 GTAC GT^AC SexAI 647 ACCAGGT A^CC[AT]GGT SmlI 799 CTCAAG C^T[CT][GA]AG TfiI 229 GATTC G^A[AT]TC Tru9I 1694 TTAA T^TAA Van91I 678 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 80 CCACGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 311 GGCGCC G[GA]^CG[CT]C 1130 GGCGCC AvaI 131 CCCGGG C^[CT]CG[GA]G 1020 CCCGGG BbeI 311 GGCGCC GGCGC^C 1130 GGCGCC BbvCI 1327 GCTGAGG CCTCAGC(-5/-2) 1419 GCTGAGG BsaHI 311 GGCGCC G[GA]^CG[CT]C 1130 GGCGCC BsoBI 131 CCCGGG C^[CT]CG[GA]G 1020 CCCGGG BstAPI 1176 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1707 GCAGAGGGTGC BstF5I 1632 CATCC GGATG(2/0) 1530 GGATG BstYI 39 GGATCT [GA]^GATC[CT] 1538 AGATCC DraIII 120 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG 497 CACAGGGTG FokI 1632 CATCC GGATG(9/13) 1530 GGATG Hin1I 311 GGCGCC G[GA]^CG[CT]C 1130 GGCGCC KasI 311 GGCGCC G^GCGCC 1130 GGCGCC KspI 55 CCGCGG CCGC^GG 202 CCGCGG MflI 39 GGATCT [GA]^GATC[CT] 1538 AGATCC NarI 311 GGCGCC GG^CGCC 1130 GGCGCC NlaIII 1231 CATG CATG^ 1308 CATG PpuMI 722 AGGTCCT [GA]G^G[AT]CC[CT] 1301 AGGTCCT SacII 55 CCGCGG CCGC^GG 202 CCGCGG SapI 729 GAAGAGC GCTCTTC(1/4) 1444 GAAGAGC SfoI 311 GGCGCC GGC^GCC 1130 GGCGCC SmaI 131 CCCGGG CCC^GGG 1020 CCCGGG StuI 845 AGGCCT AGG^CCT 1772 AGGCCT Tsp45I 1556 GTCAC ^GT[GC]AC 1667 GTCAC Tsp509I 1285 AATT ^AATT 1512 AATT XhoII 39 GGATCT [GA]^GATC[CT] 1538 AGATCC XmaCI 131 CCCGGG C^CCGGG 1020 CCCGGG XmaI 131 CCCGGG C^CCGGG 1020 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AocI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BssHII BssKI BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI