Restriction map for
pF1KB9885
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BbsI BciVI BclI
BpuAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BstAPI BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflII 841 CTTAAG C^TTAAG
AvaI 809 CTCGGG C^[CT]CG[GA]G
AvrII 1006 CCTAGG C^CTAGG
BanI 1573 GGCACC G^G[CT][GA]CC
BcgI 1677 GCAGCTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 841 CTTAAG C^TTAAG
BlnI 1006 CCTAGG C^CTAGG
BsaAI 1132 TACGTG [CT]AC^GT[GA]
BsaWI 1854 TCCGGT [AT]^CCGG[AT]
BsmI 55 GAATGC GAATGC(1/-1)
BsoBI 809 CTCGGG C^[CT]CG[GA]G
Bsp1407I 400 TGTACA T^GTACA
BsrBI 430 GAGCGG CCGCTC(-3/-3)
BsrGI 400 TGTACA T^GTACA
BssSI 1754 CTCGTG CACGAG(-5/-1)
Bst1107I 1181 GTATAC GTA^TAC
BstZ17I 1181 GTATAC GTA^TAC
CfoI 576 GCGC GCG^C
DraIII 1378 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1844 GACTTACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 85 GAAGAG CTCTTC(1/4)
EcoNI 1388 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 576 GCGC GCG^C
HinP1I 576 GCGC G^CGC
Ksp632I 85 GAAGAG CTCTTC(1/4)
NspI 1739 GCATGT [GA]CATG^[CT]
PleI 73 GAGTC GAGTC(4/5)
PshAI 1777 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 85 GAAGAGC GCTCTTC(1/4)
ScaI 1152 AGTACT AGT^ACT
SfiI 921 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1155 ACTAGT A^CTAGT
SspBI 400 TGTACA T^GTACA
StuI 1599 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1181 GTATAC GT^[AC][GT]AC
1579 GTCTAC
AcsI 1549 AAATTT [GA]^AATT[CT]
1793 AAATTC
AcyI 314 GGCGTC G[GA]^CG[CT]C
434 GGCGTC
AfaI 401 GTAC GT^AC
1153 GTAC
AlwI 1501 GGATC GGATC(4/5)
1908 GGATC
ApoI 1549 AAATTT [GA]^AATT[CT]
1793 AAATTC
Asp700I 609 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1943 GAAGATATTC
BbvCI 424 GCTGAGG CCTCAGC(-5/-2)
1567 GCTGAGG
BglI 922 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1229 GCCCCAGTGGC
BglII 1272 AGATCT A^GATCT
1922 AGATCT
BsaHI 314 GGCGTC G[GA]^CG[CT]C
434 GGCGTC
BsgI 109 CTGCAC GTGCAG(16/14)
697 GTGCAG
BspMI 677 ACCTGC ACCTGC(4/8)
1017 ACCTGC
BstXI 62 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
555 CCAGGAGTATGG
BstYI 1272 AGATCT [GA]^GATC[CT]
1922 AGATCT
Hin1I 314 GGCGTC G[GA]^CG[CT]C
434 GGCGTC
HincII 456 GTTAAC GT[CT]^[GA]AC
901 GTTAAC
HindII 456 GTTAAC GT[CT]^[GA]AC
901 GTTAAC
HpaI 456 GTTAAC GTT^AAC
901 GTTAAC
MaeII 905 ACGT A^CGT
1133 ACGT
MflI 1272 AGATCT [GA]^GATC[CT]
1922 AGATCT
MslI 63 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1740 CATGTGTGTG
NcoI 923 CCATGG C^CATGG
1414 CCATGG
PflMI 135 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCACTGCGTGG
PpuMI 524 AGGACCT [GA]G^G[AT]CC[CT]
1032 GGGTCCT
PstI 5 CTGCAG CTGCA^G
487 CTGCAG
RsaI 401 GTAC GT^AC
1153 GTAC
TaqI 1497 TCGA T^CGA
1836 TCGA
TfiI 913 GATTC G^A[AT]TC
1614 GAATC
TthHB8I 1497 TCGA T^CGA
1836 TCGA
Van91I 135 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCACTGCGTGG
XhoII 1272 AGATCT [GA]^GATC[CT]
1922 AGATCT
XmnI 609 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1943 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwNI AocI AspHI
AvaII BalI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HindIII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI