Restriction map for
pF1KB9891
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HapII HgaI
Hin1I HindIII HpaII KasI KpnI
KspI MaeII MamI MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 372 ACATGT A^C[GA][CT]GT
Alw44I 1233 GTGCAC G^TGCAC
AlwNI 806 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1233 GTGCAC G^TGCAC
Asp700I 1146 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1233 GTGCAC G[AT]GC[AT]^C
AvaII 1330 GGACC G^G[AT]CC
BamHI 1369 GGATCC G^GATCC
BanI 1103 GGCACC G^G[CT][GA]CC
BanII 1074 GAGCCC G[GA]GC[CT]^C
BbvI 1171 GCTGC GCAGC(8/12)
BfaI 1226 CTAG C^TAG
BglII 1148 AGATCT A^GATCT
BsaI 326 GGTCTC GGTCTC(1/5)
BsaJI 968 CCACGG C^C[ACGT][ACGT]GG
BsgI 1069 GTGCAG GTGCAG(16/14)
BsiHKAI 1233 GTGCAC G[AT]GC[AT]^C
BsiYI 661 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 661 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 340 CGTCTC CGTCTC(1/5)
BsmFI 255 GGGAC GGGAC(10/14)
BspHI 420 TCATGA T^CATGA
BspLU11I 372 ACATGT A^CATGT
BspMI 810 ACCTGC ACCTGC(4/8)
Cfr13I 1330 GGACC G^G[ACGT]CC
DsaI 968 CCACGG C^C[GA][CT]GG
EcoT22I 872 ATGCAT ATGCA^T
HaeII 395 GGCGCT [GA]GCGC^[CT]
HincII 955 GTTAAC GT[CT]^[GA]AC
HindII 955 GTTAAC GT[CT]^[GA]AC
HinfI 711 GAATC G^A[ACGT]TC
HpaI 955 GTTAAC GTT^AAC
MaeI 1226 CTAG C^TAG
MspA1I 62 CAGCTG C[AC]G^C[GT]G
NsiI 872 ATGCAT ATGCA^T
PflMI 661 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 62 CAGCTG CAG^CTG
RcaI 420 TCATGA T^CATGA
SapI 1174 GCTCTTC GCTCTTC(1/4)
Sau96I 1330 GGACC G^G[ACGT]CC
ScaI 1297 AGTACT AGT^ACT
SexAI 322 ACCAGGT A^CC[AT]GGT
SfaNI 874 GCATC GCATC(5/9)
StuI 297 AGGCCT AGG^CCT
TfiI 711 GAATC G^A[AT]TC
TseI 1171 GCTGC G^C[AT]GC
Tsp45I 698 GTGAC ^GT[GC]AC
Van91I 661 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1146 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 263 GTAC GT^AC
1298 GTAC
BciVI 288 GGATAC GTATCC(6/5)
349 GTATCC
CfoI 396 GCGC GCG^C
1008 GCGC
Fnu4HI 24 GCCGC GC^[ACGT]GC
1171 GCTGC
HaeIII 298 GGCC GG^CC
401 GGCC
HhaI 396 GCGC GCG^C
1008 GCGC
HinP1I 396 GCGC G^CGC
1008 GCGC
HphI 144 GGTGA GGTGA(8/7)
194 TCACC
ItaI 24 GCCGC GC^[ACGT]GC
1171 GCTGC
MfeI 434 CAATTG C^AATTG
1080 CAATTG
MunI 434 CAATTG C^AATTG
1080 CAATTG
NlaIV 1103 GGCACC GG[ACGT]^[ACGT]CC
1369 GGATCC
NspI 372 ACATGT [GA]CATG^[CT]
1394 GCATGT
RsaI 263 GTAC GT^AC
1298 GTAC
SfcI 893 CTGTAG C^T[GA][CT]AG
1336 CTACAG
TaqI 357 TCGA T^CGA
826 TCGA
TthHB8I 357 TCGA T^CGA
826 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BmyI BpmI BseRI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EarI EcoRII
FokI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII Sau3AI ScrFI
SmlI Tru9I Tsp509I TspRI XhoII