Restriction map for
pF1KF0017
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# NO CUT ENZYMES ( 123 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 274 GACGTC GACGT^C
Acc65I 334 GGTACC G^GTACC
AflIII 557 ACGTGT A^C[GA][CT]GT
Aor51HI 632 AGCGCT AGC^GCT
Asp718I 334 GGTACC G^GTACC
AspI 1141 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 679 CCCGAG C^[CT]CG[GA]G
BamHI 354 GGATCC G^GATCC
BciVI 236 GTATCC GTATCC(6/5)
BlpI 30 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
BsaAI 556 TACGTG [CT]AC^GT[GA]
BsaI 1146 GGTCTC GGTCTC(1/5)
BsaWI 482 ACCGGA [AT]^CCGG[AT]
BseRI 160 GAGGAG GAGGAG(10/8)
BsgI 928 GTGCAG GTGCAG(16/14)
BsmBI 43 GAGACG CGTCTC(1/5)
BsoBI 679 CCCGAG C^[CT]CG[GA]G
BssSI 397 CACGAG CACGAG(-5/-1)
BstAPI 255 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 354 GGATCC [GA]^GATC[CT]
CelII 30 GCTGAGC GC^T[ACGT]AGC
Eco47III 632 AGCGCT AGC^GCT
EcoT14I 230 CCAAGG C^C[AT][AT]GG
HgaI 530 GCGTC GACGC(5/10)
KpnI 334 GGTACC GGTAC^C
MflI 354 GGATCC [GA]^GATC[CT]
MslI 573 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1141 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1102 GAGTC GAGTC(4/5)
PpuMI 371 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 367 CTGCAG CTGCA^G
PvuII 688 CAGCTG CAG^CTG
SacI 967 GAGCTC GAGCT^C
SbfI 366 CCTGCAGG CCTGCA^GG
SexAI 1142 ACCTGGT A^CC[AT]GGT
SfcI 367 CTGCAG C^T[GA][CT]AG
Sse8387I 366 CCTGCAGG CCTGCA^GG
StyI 230 CCAAGG C^C[AT][AT]GG
TfiI 1051 GAATC G^A[AT]TC
Tsp45I 1088 GTGAC ^GT[GC]AC
Tsp509I 903 AATT ^AATT
Tth111I 1141 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 354 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 274 GACGTC G[GA]^CG[CT]C
529 GGCGTC
AfaI 335 GTAC GT^AC
1056 GTAC
AocI 429 CCTCAGG CC^T[ACGT]AGG
736 CCTGAGG
Asp700I 696 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
951 GAAGCCATTC
AspHI 253 GAGCAC G[AT]GC[AT]^C
967 GAGCTC
BanI 71 GGCACC G^G[CT][GA]CC
334 GGTACC
BanII 19 GAGCCC G[GA]GC[CT]^C
967 GAGCTC
BbsI 819 GAAGAC GAAGAC(2/6)
532 GTCTTC
BfaI 805 CTAG C^TAG
980 CTAG
BpuAI 819 GAAGAC GAAGAC(2/6)
532 GTCTTC
BsaHI 274 GACGTC G[GA]^CG[CT]C
529 GGCGTC
BsiHKAI 253 GAGCAC G[AT]GC[AT]^C
967 GAGCTC
BsmAI 43 GAGAC GTCTC(1/5)
1147 GTCTC
BsmFI 614 GGGAC GGGAC(10/14)
1129 GTCCC
BsrDI 285 CATTGC GCAATG(2/0)
749 GCAATG
Bsu36I 429 CCTCAGG CC^T[ACGT]AGG
736 CCTGAGG
EarI 700 CTCTTC CTCTTC(1/4)
49 GAAGAG
Eco81I 429 CCTCAGG CC^T[ACGT]AGG
736 CCTGAGG
EcoNI 417 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
675 CCTGCCCGAGG
HaeII 632 AGCGCT [GA]GCGC^[CT]
833 GGCGCT
Hin1I 274 GACGTC G[GA]^CG[CT]C
529 GGCGTC
HinfI 1051 GAATC G^A[ACGT]TC
1102 GAGTC
HphI 878 TCACC GGTGA(8/7)
1121 TCACC
Ksp632I 700 CTCTTC CTCTTC(1/4)
49 GAAGAG
KspI 23 CCGCGG CCGC^GG
651 CCGCGG
MaeI 805 CTAG C^TAG
980 CTAG
MaeIII 54 GTTAC ^GT[ACGT]AC
1088 GTGAC
RsaI 335 GTAC GT^AC
1056 GTAC
SacII 23 CCGCGG CCGC^GG
651 CCGCGG
SmlI 799 CTCAAG C^T[CT][GA]AG
913 CTTGAG
XmnI 696 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
951 GAAGCCATTC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI TspRI TthHB8I