Restriction map for
pF1KF0034
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII BbrPI BcgI BclI
BfrI BlpI Bpu1102I BsaAI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI EcoRI EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI ScaI SfuI SnaBI SpeI
SspBI SspI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AcsI 2593 AAATTC [GA]^AATT[CT]
AhdI 1333 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2593 AAATTC [GA]^AATT[CT]
AspEI 1333 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1386 CCTAGG C^CTAGG
BamHI 312 GGATCC G^GATCC
BciVI 321 GGATAC GTATCC(6/5)
BglII 3746 AGATCT A^GATCT
BlnI 1386 CCTAGG C^CTAGG
BsaBI 3613 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 2019 CTCGTG CACGAG(-5/-1)
DraIII 2016 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1333 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 676 GATATC GAT^ATC
MamI 3613 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 3260 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2697 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2453 GCTCTTC GCTCTTC(1/4)
SexAI 3794 ACCTGGT A^CC[AT]GGT
SgrAI 1045 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1347 GCATGC GCATG^C
Tsp509I 2594 AATT ^AATT
Van91I 3260 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccIII 729 TCCGGA T^CCGGA
2967 TCCGGA
BsaWI 729 TCCGGA [AT]^CCGG[AT]
2967 TCCGGA
BseAI 729 TCCGGA T^CCGGA
2967 TCCGGA
BsmBI 448 CGTCTC CGTCTC(1/5)
539 GAGACG
BspEI 729 TCCGGA T^CCGGA
2967 TCCGGA
BsrI 2434 CCAGT ACTGG(1/-1)
3778 CCAGT
DrdI 30 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2883 GACTTCCTGGTC
MaeII 3562 ACGT A^CGT
3809 ACGT
MroI 729 TCCGGA T^CCGGA
2967 TCCGGA
MslI 2112 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2652 CACTGAGATG
NspI 371 ACATGC [GA]CATG^[CT]
1347 GCATGC
PaeR7I 20 CTCGAG C^TCGAG
1872 CTCGAG
SbfI 138 CCTGCAGG CCTGCA^GG
1374 CCTGCAGG
Sse8387I 138 CCTGCAGG CCTGCA^GG
1374 CCTGCAGG
StuI 707 AGGCCT AGG^CCT
864 AGGCCT
XcmI 3299 CCAGCGGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3753 CCACAGCAAAAGTGG
XhoI 20 CTCGAG C^TCGAG
1872 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI