Restriction map for
pF1KF0052
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BglI BlnI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HgaI
Hin1I KasI MaeII MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfoI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 803 GGTACC G^GTACC
AcsI 412 GAATTT [GA]^AATT[CT]
AflII 676 CTTAAG C^TTAAG
AlwNI 847 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 412 GAATTT [GA]^AATT[CT]
Asp718I 803 GGTACC G^GTACC
AspHI 715 GAGCAC G[AT]GC[AT]^C
BanI 803 GGTACC G^G[CT][GA]CC
BanII 952 GGGCTC G[GA]GC[CT]^C
BciVI 326 GGATAC GTATCC(6/5)
BclI 863 TGATCA T^GATCA
BfrI 676 CTTAAG C^TTAAG
BglII 887 AGATCT A^GATCT
BsaBI 921 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 690 GAGACC GGTCTC(1/5)
BsiHKAI 715 GAGCAC G[AT]GC[AT]^C
BsmAI 690 GAGAC GTCTC(1/5)
BsmFI 577 GTCCC GGGAC(10/14)
BsmI 333 GAATGC GAATGC(1/-1)
BspHI 539 TCATGA T^CATGA
BspMI 603 ACCTGC ACCTGC(4/8)
BstAPI 252 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 757 TTCGAA TT^CGAA
BstXI 821 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 863 TGATCA T^GATCA
HincII 630 GTTAAC GT[CT]^[GA]AC
HindII 630 GTTAAC GT[CT]^[GA]AC
HindIII 673 AAGCTT A^AGCTT
HpaI 630 GTTAAC GTT^AAC
KpnI 803 GGTACC GGTAC^C
MamI 921 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 915 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 757 TTCGAA TT^CGAA
RcaI 539 TCATGA T^CATGA
ScaI 62 AGTACT AGT^ACT
SfcI 872 CTGTAG C^T[GA][CT]AG
SfuI 757 TTCGAA TT^CGAA
SgrAI 967 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 758 TCGA T^CGA
TthHB8I 758 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 58 CCCGAG C^[CT]CG[GA]G
70 CCCGAG
BcnI 24 CCCGG CC^[GC]GG
30 CCCGG
BlpI 454 GCTGAGC GC^T[ACGT]AGC
974 GCTCAGC
BmyI 715 GAGCAC G[AGT]GC[ACT]^C
952 GGGCTC
Bpu1102I 454 GCTGAGC GC^T[ACGT]AGC
974 GCTCAGC
BseRI 73 GAGGAG GAGGAG(10/8)
594 GAGGAG
BsoBI 58 CCCGAG C^[CT]CG[GA]G
70 CCCGAG
Bsp1286I 715 GAGCAC G[AGT]GC[ACT]^C
952 GGGCTC
BsrFI 51 GCCGGC [GA]^CCGG[CT]
968 GCCGGC
BsrI 228 ACTGG ACTGG(1/-1)
511 ACTGG
CelII 454 GCTGAGC GC^T[ACGT]AGC
974 GCTCAGC
Cfr10I 51 GCCGGC [GA]^CCGG[CT]
968 GCCGGC
DsaI 8 CCGCGG C^C[GA][CT]GG
734 CCGCGG
KspI 8 CCGCGG CCGC^GG
734 CCGCGG
MaeIII 681 GTCAC ^GT[ACGT]AC
908 GTGAC
NaeI 51 GCCGGC GCC^GGC
968 GCCGGC
NciI 24 CCCGG CC^[GC]GG
30 CCCGG
NgoMIV 51 GCCGGC G^CCGGC
968 GCCGGC
PleI 130 GAGTC GAGTC(4/5)
832 GAGTC
SacII 8 CCGCGG CCGC^GG
734 CCGCGG
SapI 82 GAAGAGC GCTCTTC(1/4)
954 GCTCTTC
SfaNI 151 GATGC GCATC(5/9)
697 GCATC
SmlI 238 CTCAAG C^T[CT][GA]AG
676 CTTAAG
TfiI 517 GATTC G^A[AT]TC
739 GAATC
Tsp45I 681 GTCAC ^GT[GC]AC
908 GTGAC
Tsp509I 379 AATT ^AATT
413 AATT
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
404 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI Tru9I TseI XhoII