Restriction map for
pF1KF0162
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AvaI AviII AvrII BamHI
BbeI BbrPI BclI BfrI BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspMI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CfoI ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1261 GTCTAC GT^[AC][GT]AC
AccII 1913 CGCG CG^CG
AcyI 757 GACGCC G[GA]^CG[CT]C
AhdI 1744 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1960 CCTCAGG CC^T[ACGT]AGG
ApaI 1396 GGGCCC GGGCC^C
AspEI 1744 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1548 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1664 TGGCCA TGG^CCA
BbsI 1545 GAAGAC GAAGAC(2/6)
BbvCI 1695 CCTCAGC CCTCAGC(-5/-2)
BcgI 2001 CGAGATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1369 GGATAC GTATCC(6/5)
BglI 1843 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 213 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC
BpuAI 1545 GAAGAC GAAGAC(2/6)
BsaHI 757 GACGCC G[GA]^CG[CT]C
BsaI 25 GAGACC GGTCTC(1/5)
BsgI 427 CTGCAC GTGCAG(16/14)
BsmBI 333 CGTCTC CGTCTC(1/5)
BspHI 890 TCATGA T^CATGA
BspLU11I 1106 ACATGT A^CATGT
BsrBI 1562 CCGCTC CCGCTC(-3/-3)
BsrDI 2007 CATTGC GCAATG(2/0)
BstUI 1913 CGCG CG^CG
Bsu36I 1960 CCTCAGG CC^T[ACGT]AGG
CelII 213 GCTCAGC GC^T[ACGT]AGC
DrdI 517 GACGCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1744 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1960 CCTCAGG CC^T[ACGT]AGG
Hin1I 757 GACGCC G[GA]^CG[CT]C
MluNI 1664 TGGCCA TGG^CCA
MscI 1664 TGGCCA TGG^CCA
MseI 1580 TTAA T^TAA
MvnI 1913 CGCG CG^CG
NcoI 1599 CCATGG C^CATGG
PflFI 1548 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1925 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 327 CTGCAG CTGCA^G
RcaI 890 TCATGA T^CATGA
SapI 2026 GAAGAGC GCTCTTC(1/4)
SfcI 327 CTGCAG C^T[GA][CT]AG
SfiI 1842 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 473 CTCAAG C^T[CT][GA]AG
SphI 977 GCATGC GCATG^C
Tru9I 1580 TTAA T^TAA
Tsp509I 453 AATT ^AATT
Tth111I 1548 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2034 TCTAGA T^CTAGA
XcmI 1403 CCACAGATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 98 TCCGGA T^CCGGA
1208 TCCGGA
AflIII 1106 ACATGT A^C[GA][CT]GT
1146 ACGTGT
BanI 1100 GGTGCC G^G[CT][GA]CC
1966 GGTGCC
BglII 1214 AGATCT A^GATCT
1976 AGATCT
BsaWI 98 TCCGGA [AT]^CCGG[AT]
1208 TCCGGA
BseAI 98 TCCGGA T^CCGGA
1208 TCCGGA
BspEI 98 TCCGGA T^CCGGA
1208 TCCGGA
BsrFI 1789 ACCGGC [GA]^CCGG[CT]
1824 ACCGGC
BstAPI 972 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1328 GCAACATCTGC
Cfr10I 1789 ACCGGC [GA]^CCGG[CT]
1824 ACCGGC
DraII 1395 GGGGCCC [GA]G^G[ACGT]CC[CT]
1396 GGGCCCT
DraIII 1126 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1142 CACGACGTG
EcoO109I 1395 GGGGCCC [GA]G^G[ACGT]CC[CT]
1396 GGGCCCT
EcoT14I 236 CCTTGG C^C[AT][AT]GG
1599 CCATGG
FauI 201 CCCGC CCCGC(4/6)
1899 CCCGC
MroI 98 TCCGGA T^CCGGA
1208 TCCGGA
NspI 977 GCATGC [GA]CATG^[CT]
1106 ACATGT
SacI 1486 GAGCTC GAGCT^C
1783 GAGCTC
StyI 236 CCTTGG C^C[AT][AT]GG
1599 CCATGG
TfiI 1320 GAATC G^A[AT]TC
1895 GAATC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII