Restriction map for
pF1KF0165
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 458 GGTACC G^GTACC
AccI 655 GTCTAC GT^[AC][GT]AC
AccII 676 CGCG CG^CG
AfaI 459 GTAC GT^AC
AlwNI 647 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 26 GGGCCC GGGCC^C
Asp718I 458 GGTACC G^GTACC
AvaI 100 CCCGGG C^[CT]CG[GA]G
BcgI 684 CGAGGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 160 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 259 CTCCAG CTGGAG(16/14)
BsmBI 449 GAGACG CGTCTC(1/5)
BsoBI 100 CCCGGG C^[CT]CG[GA]G
BspHI 249 TCATGA T^CATGA
BstBI 769 TTCGAA TT^CGAA
BstEII 345 GGTCACC G^GT[ACGT]ACC
BstPI 345 GGTCACC G^GT[ACGT]ACC
BstUI 676 CGCG CG^CG
CfoI 672 GCGC GCG^C
DrdI 337 GACTGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 413 CCACGG C^C[GA][CT]GG
EcoNI 744 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 345 GGTCACC G^GT[ACGT]ACC
EcoT14I 81 CCTTGG C^C[AT][AT]GG
HaeII 671 AGCGCC [GA]GCGC^[CT]
HgaI 451 GACGC GACGC(5/10)
HhaI 672 GCGC GCG^C
HinP1I 672 GCGC G^CGC
KpnI 458 GGTACC GGTAC^C
MseI 788 TTAA T^TAA
MslI 348 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 676 CGCG CG^CG
NspV 769 TTCGAA TT^CGAA
PflMI 327 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 477 GAGTC GAGTC(4/5)
PshAI 681 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 189 CAGCTG CAG^CTG
RcaI 249 TCATGA T^CATGA
RsaI 459 GTAC GT^AC
SexAI 791 ACCTGGT A^CC[AT]GGT
SfiI 159 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 769 TTCGAA TT^CGAA
SmaI 100 CCCGGG CCC^GGG
StuI 36 AGGCCT AGG^CCT
StyI 81 CCTTGG C^C[AT][AT]GG
Tru9I 788 TTAA T^TAA
Tsp509I 221 AATT ^AATT
Van91I 327 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 100 CCCGGG C^CCGGG
XmaI 100 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 610 GATCC GGATC(4/5)
197 GGATC
BalI 167 TGGCCA TGG^CCA
403 TGGCCA
BglII 255 AGATCT A^GATCT
616 AGATCT
BsaI 679 GAGACC GGTCTC(1/5)
756 GAGACC
BseRI 76 CTCCTC GAGGAG(10/8)
270 GAGGAG
BsmFI 103 GGGAC GGGAC(10/14)
264 GGGAC
BsrFI 142 ACCGGC [GA]^CCGG[CT]
508 ACCGGC
Cfr10I 142 ACCGGC [GA]^CCGG[CT]
508 ACCGGC
MaeIII 133 GTGAC ^GT[ACGT]AC
346 GTCAC
MboII 253 GAAGA GAAGA(8/7)
772 GAAGA
MluNI 167 TGGCCA TGG^CCA
403 TGGCCA
MscI 167 TGGCCA TGG^CCA
403 TGGCCA
MspA1I 189 CAGCTG C[AC]G^C[GT]G
426 CAGCGG
NlaIII 156 CATG CATG^
250 CATG
PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT]
587 AGGACCC
PstI 192 CTGCAG CTGCA^G
667 CTGCAG
SfcI 192 CTGCAG C^T[GA][CT]AG
667 CTGCAG
TaqI 701 TCGA T^CGA
770 TCGA
TfiI 649 GAATC G^A[AT]TC
767 GATTC
Tsp45I 133 GTGAC ^GT[GC]AC
346 GTCAC
TthHB8I 701 TCGA T^CGA
770 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI TspRI
XhoII