Restriction map for
pF1KF0267
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbeI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BssHII BstAPI BstBI
BstEII BstPI BstUI ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HpaI KasI KspI
MaeIII MamI MluI MroI MseI
MvnI NarI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tsp45I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 2417 GGTACC G^GTACC
AccI 2223 GTATAC GT^[AC][GT]AC
AclI 1309 AACGTT AA^CGTT
AflIII 627 ACACGT A^C[GA][CT]GT
AhdI 1498 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 374 AGCGCT AGC^GCT
Asp718I 2417 GGTACC G^GTACC
AspEI 1498 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 628 CACGTG CAC^GTG
BbsI 2452 GAAGAC GAAGAC(2/6)
BlpI 1987 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1987 GCTGAGC GC^T[ACGT]AGC
BpuAI 2452 GAAGAC GAAGAC(2/6)
BsaAI 628 CACGTG [CT]AC^GT[GA]
BsmBI 267 CGTCTC CGTCTC(1/5)
Bsp1407I 2355 TGTACA T^GTACA
BspHI 443 TCATGA T^CATGA
BsrGI 2355 TGTACA T^GTACA
BssSI 1166 CACGAG CACGAG(-5/-1)
Bst1107I 2223 GTATAC GTA^TAC
BstZ17I 2223 GTATAC GTA^TAC
CelII 1987 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1498 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 374 AGCGCT AGC^GCT
HaeII 374 AGCGCT [GA]GCGC^[CT]
HindIII 193 AAGCTT A^AGCTT
KpnI 2417 GGTACC GGTAC^C
MfeI 361 CAATTG C^AATTG
MunI 361 CAATTG C^AATTG
NaeI 2906 GCCGGC GCC^GGC
NdeI 3257 CATATG CA^TATG
NgoMIV 2906 GCCGGC G^CCGGC
PmaCI 628 CACGTG CAC^GTG
PmlI 628 CACGTG CAC^GTG
Psp1406I 1309 AACGTT AA^CGTT
RcaI 443 TCATGA T^CATGA
SapI 1948 GAAGAGC GCTCTTC(1/4)
SspBI 2355 TGTACA T^GTACA
XcmI 2283 CCAGGTAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 124 GAATTT [GA]^AATT[CT]
205 GAATTT
ApaI 1036 GGGCCC GGGCC^C
2315 GGGCCC
ApoI 124 GAATTT [GA]^AATT[CT]
205 GAATTT
BamHI 306 GGATCC G^GATCC
3181 GGATCC
BsrDI 1061 CATTGC GCAATG(2/0)
2854 GCAATG
DraIII 989 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1133 CACTGGGTG
EcoT22I 490 ATGCAT ATGCA^T
3260 ATGCAT
HincII 1252 GTCAAC GT[CT]^[GA]AC
2614 GTTGAC
HindII 1252 GTCAAC GT[CT]^[GA]AC
2614 GTTGAC
MaeII 629 ACGT A^CGT
1310 ACGT
NsiI 490 ATGCAT ATGCA^T
3260 ATGCAT
PflMI 509 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1165 CCACGAGCTGG
PvuII 1001 CAGCTG CAG^CTG
1758 CAGCTG
SacI 1663 GAGCTC GAGCT^C
3151 GAGCTC
SmaI 704 CCCGGG CCC^GGG
3011 CCCGGG
TaqI 818 TCGA T^CGA
2955 TCGA
TfiI 1087 GATTC G^A[AT]TC
2868 GATTC
TthHB8I 818 TCGA T^CGA
2955 TCGA
Van91I 509 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1165 CCACGAGCTGG
XmaCI 704 CCCGGG C^CCGGG
3011 CCCGGG
XmaI 704 CCCGGG C^CCGGG
3011 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TseI
Tsp509I TspRI XhoII