Restriction map for
pF1KF0293
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# NO CUT ENZYMES ( 68 enzymes) #
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AclI AcsI AflII AflIII ApoI
AscI AseI AsnI AviII AvrII
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsiWI BsmI BspDI
BspLU11I BssHII BstBI ClaI CpoI
DraI DrdI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MaeI MamI MluI MseI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I RsrII SalI SapI
SbfI ScaI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1567 GACGTC GACGT^C
Acc65I 194 GGTACC G^GTACC
AccI 679 GTATAC GT^[AC][GT]AC
AgeI 272 ACCGGT A^CCGGT
AhdI 2512 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 721 GTGCAC G^TGCAC
AocI 178 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2014 AGCGCT AGC^GCT
ApaLI 721 GTGCAC G^TGCAC
Asp700I 2634 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 194 GGTACC G^GTACC
AspEI 2512 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1162 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1140 GGATCC G^GATCC
BbeI 2963 GGCGCC GGCGC^C
BbrPI 583 CACGTG CAC^GTG
BbsI 321 GAAGAC GAAGAC(2/6)
BcgI 2466 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 321 GAAGAC GAAGAC(2/6)
BsaI 2774 GAGACC GGTCTC(1/5)
Bsp1407I 1640 TGTACA T^GTACA
BspHI 74 TCATGA T^CATGA
BsrGI 1640 TGTACA T^GTACA
Bst1107I 679 GTATAC GTA^TAC
BstEII 1704 GGTGACC G^GT[ACGT]ACC
BstPI 1704 GGTGACC G^GT[ACGT]ACC
BstXI 1352 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 679 GTATAC GTA^TAC
Bsu36I 178 CCTGAGG CC^T[ACGT]AGG
DraIII 2236 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2839 CGGCCG C^GGCCG
Eam1105I 2512 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2839 CGGCCG C^GGCCG
Eco47III 2014 AGCGCT AGC^GCT
Eco52I 2839 CGGCCG C^GGCCG
Eco81I 178 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1704 GGTGACC G^GT[ACGT]ACC
HgaI 2924 GCGTC GACGC(5/10)
HincII 1210 GTCAAC GT[CT]^[GA]AC
HindII 1210 GTCAAC GT[CT]^[GA]AC
KasI 2963 GGCGCC G^GCGCC
KpnI 194 GGTACC GGTAC^C
KspI 2799 CCGCGG CCGC^GG
MfeI 616 CAATTG C^AATTG
MunI 616 CAATTG C^AATTG
NarI 2963 GGCGCC GG^CGCC
NspI 2423 ACATGC [GA]CATG^[CT]
PflFI 1162 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 272 ACCGGT A^CCGGT
PmaCI 583 CACGTG CAC^GTG
PmlI 583 CACGTG CAC^GTG
PshAI 2091 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 527 CGATCG CGAT^CG
RcaI 74 TCATGA T^CATGA
SacI 208 GAGCTC GAGCT^C
SacII 2799 CCGCGG CCGC^GG
SexAI 1859 ACCAGGT A^CC[AT]GGT
SfiI 2439 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2963 GGCGCC GGC^GCC
SspBI 1640 TGTACA T^GTACA
Tth111I 1162 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2634 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 1567 GACGTC G[GA]^CG[CT]C
2963 GGCGCC
BalI 242 TGGCCA TGG^CCA
2058 TGGCCA
BsaAI 583 CACGTG [CT]AC^GT[GA]
1572 CACGTA
BsaHI 1567 GACGTC G[GA]^CG[CT]C
2963 GGCGCC
BsiEI 527 CGATCG CG[GA][CT]^CG
2839 CGGCCG
BsmBI 2925 CGTCTC CGTCTC(1/5)
865 GAGACG
BsrDI 262 GCAATG GCAATG(2/0)
446 GCAATG
BsrFI 272 ACCGGT [GA]^CCGG[CT]
2705 ACCGGC
BssSI 961 CTCGTG CACGAG(-5/-1)
2815 CTCGTG
Cfr10I 272 ACCGGT [GA]^CCGG[CT]
2705 ACCGGC
Hin1I 1567 GACGTC G[GA]^CG[CT]C
2963 GGCGCC
MluNI 242 TGGCCA TGG^CCA
2058 TGGCCA
MscI 242 TGGCCA TGG^CCA
2058 TGGCCA
PflMI 1983 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2778 CCAGCTGGTGG
PleI 707 GACTC GAGTC(4/5)
808 GAGTC
StuI 437 AGGCCT AGG^CCT
1409 AGGCCT
Tsp509I 617 AATT ^AATT
685 AATT
Van91I 1983 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2778 CCAGCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MroI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI