Restriction map for
pF1KB7657
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NcoI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 677 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 73 AGCGCT AGC^GCT
Asp700I 948 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 670 GAGCTC G[GA]GC[CT]^C
BbeI 269 GGCGCC GGCGC^C
BglI 220 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 623 AGATCT A^GATCT
BsaI 871 GAGACC GGTCTC(1/5)
BsmBI 772 GAGACG CGTCTC(1/5)
BsrBI 23 GAGCGG CCGCTC(-3/-3)
BsrI 339 ACTGG ACTGG(1/-1)
BssHII 34 GCGCGC G^CGCGC
BstAPI 90 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 140 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 825 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 73 AGCGCT AGC^GCT
EcoO109I 825 GGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 136 GACGC GACGC(5/10)
KasI 269 GGCGCC G^GCGCC
MaeII 775 ACGT A^CGT
MslI 244 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 269 GGCGCC GG^CGCC
NdeI 763 CATATG CA^TATG
NlaIII 711 CATG CATG^
NruI 78 TCGCGA TCG^CGA
SacI 670 GAGCTC GAGCT^C
SbfI 105 CCTGCAGG CCTGCA^GG
ScaI 632 AGTACT AGT^ACT
SfaNI 243 GCATC GCATC(5/9)
SfoI 269 GGCGCC GGC^GCC
SmlI 205 CTCAAG C^T[CT][GA]AG
Sse8387I 105 CCTGCAGG CCTGCA^GG
TaqI 701 TCGA T^CGA
TthHB8I 701 TCGA T^CGA
XmnI 948 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 136 GACGCC G[GA]^CG[CT]C
269 GGCGCC
AfaI 633 GTAC GT^AC
777 GTAC
AgeI 55 ACCGGT A^CCGGT
396 ACCGGT
BsaHI 136 GACGCC G[GA]^CG[CT]C
269 GGCGCC
BsaWI 55 ACCGGT [AT]^CCGG[AT]
396 ACCGGT
BseRI 724 GAGGAG GAGGAG(10/8)
868 GAGGAG
BspMI 704 ACCTGC ACCTGC(4/8)
369 GCAGGT
BsrFI 55 ACCGGT [GA]^CCGG[CT]
396 ACCGGT
BstEII 51 GGTGACC G^GT[ACGT]ACC
399 GGTCACC
BstF5I 244 CATCC GGATG(2/0)
843 GGATG
BstPI 51 GGTGACC G^GT[ACGT]ACC
399 GGTCACC
Cfr10I 55 ACCGGT [GA]^CCGG[CT]
396 ACCGGT
DsaI 389 CCACGG C^C[GA][CT]GG
441 CCGTGG
EaeI 386 CGGCCA [CT]^GGCC[GA]
657 TGGCCG
EcoO65I 51 GGTGACC G^GT[ACGT]ACC
399 GGTCACC
FokI 244 CATCC GGATG(9/13)
843 GGATG
Hin1I 136 GACGCC G[GA]^CG[CT]C
269 GGCGCC
PinAI 55 ACCGGT A^CCGGT
396 ACCGGT
PstI 106 CTGCAG CTGCA^G
830 CTGCAG
PvuII 680 CAGCTG CAG^CTG
929 CAGCTG
RsaI 633 GTAC GT^AC
777 GTAC
SfcI 106 CTGCAG C^T[GA][CT]AG
830 CTGCAG
SmaI 152 CCCGGG CCC^GGG
221 CCCGGG
XmaCI 152 CCCGGG C^CCGGG
221 CCCGGG
XmaI 152 CCCGGG C^CCGGG
221 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BanI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI StyI
TseI Tsp45I TspRI XhoII