Restriction map for
pF1KB7910
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MboI MfeI
MflI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 924 GTGCAC G^TGCAC
AlwNI 157 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 689 AGCGCT AGC^GCT
ApaI 240 GGGCCC GGGCC^C
ApaLI 924 GTGCAC G^TGCAC
BbsI 511 GTCTTC GAAGAC(2/6)
BcgI 593 GCAAAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 311 CTAG C^TAG
BpuAI 511 GTCTTC GAAGAC(2/6)
BsaI 107 GAGACC GGTCTC(1/5)
BsgI 58 GTGCAG GTGCAG(16/14)
BsmAI 107 GAGAC GTCTC(1/5)
BsrBI 73 CCGCTC CCGCTC(-3/-3)
BsrFI 356 ACCGGC [GA]^CCGG[CT]
Cfr10I 356 ACCGGC [GA]^CCGG[CT]
EarI 21 GAAGAG CTCTTC(1/4)
Eco47III 689 AGCGCT AGC^GCT
FauI 442 CCCGC CCCGC(4/6)
HaeII 689 AGCGCT [GA]GCGC^[CT]
HgaI 548 GACGC GACGC(5/10)
Ksp632I 21 GAAGAG CTCTTC(1/4)
MaeI 311 CTAG C^TAG
MaeII 635 ACGT A^CGT
MaeIII 581 GTGAC ^GT[ACGT]AC
MslI 808 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 912 CAGCTG C[AC]G^C[GT]G
PpuMI 562 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 912 CAGCTG CAG^CTG
SacI 861 GAGCTC GAGCT^C
SapI 21 GAAGAGC GCTCTTC(1/4)
SfaNI 481 GATGC GCATC(5/9)
SfiI 216 GGCCAGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 985 CTCAAG C^T[CT][GA]AG
Tsp45I 581 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 734 CGCG CG^CG
967 CGCG
AfaI 411 GTAC GT^AC
486 GTAC
BbvI 624 GCAGC GCAGC(8/12)
653 GCAGC
BglI 217 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
407 GCCTGTACGGC
BspMI 713 ACCTGC ACCTGC(4/8)
953 ACCTGC
BstUI 734 CGCG CG^CG
967 CGCG
DraIII 490 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
607 CACGCTGTG
EcoT14I 329 CCTTGG C^C[AT][AT]GG
446 CCTTGG
HinfI 274 GACTC G^A[ACGT]TC
289 GACTC
MvnI 734 CGCG CG^CG
967 CGCG
NspI 540 GCATGT [GA]CATG^[CT]
668 GCATGT
PflMI 156 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCAGAACTTGG
PleI 274 GACTC GAGTC(4/5)
289 GACTC
RsaI 411 GTAC GT^AC
486 GTAC
StuI 680 AGGCCT AGG^CCT
848 AGGCCT
StyI 329 CCTTGG C^C[AT][AT]GG
446 CCTTGG
TaqI 533 TCGA T^CGA
659 TCGA
TseI 624 GCAGC G^C[AT]GC
653 GCAGC
TthHB8I 533 TCGA T^CGA
659 TCGA
Van91I 156 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCAGAACTTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanI BanII BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TspRI