Restriction map for
pF1KB7951
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KspI MaeIII MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 719 GGTACC G^GTACC
AcyI 163 GGCGCC G[GA]^CG[CT]C
ApaI 608 GGGCCC GGGCC^C
Asp718I 719 GGTACC G^GTACC
AvaI 270 CCCGGG C^[CT]CG[GA]G
AvaII 306 GGACC G^G[AT]CC
BalI 596 TGGCCA TGG^CCA
BbeI 163 GGCGCC GGCGC^C
BfaI 457 CTAG C^TAG
BglII 518 AGATCT A^GATCT
BlpI 173 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 173 GCTCAGC GC^T[ACGT]AGC
BsaHI 163 GGCGCC G[GA]^CG[CT]C
BsaWI 431 ACCGGA [AT]^CCGG[AT]
BsgI 259 GTGCAG GTGCAG(16/14)
BsoBI 270 CCCGGG C^[CT]CG[GA]G
BsrI 407 CCAGT ACTGG(1/-1)
CelII 173 GCTCAGC GC^T[ACGT]AGC
DraIII 711 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 108 CCACGG C^C[GA][CT]GG
EaeI 596 TGGCCA [CT]^GGCC[GA]
Hin1I 163 GGCGCC G[GA]^CG[CT]C
HincII 214 GTCAAC GT[CT]^[GA]AC
HindII 214 GTCAAC GT[CT]^[GA]AC
HphI 349 GGTGA GGTGA(8/7)
KasI 163 GGCGCC G^GCGCC
KpnI 719 GGTACC GGTAC^C
MaeI 457 CTAG C^TAG
MaeII 212 ACGT A^CGT
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
MslI 333 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 196 CCGCTG C[AC]G^C[GT]G
NarI 163 GGCGCC GG^CGCC
PpuMI 305 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 507 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 478 GAGCTC GAGCT^C
SapI 327 GAAGAGC GCTCTTC(1/4)
SfaNI 235 GCATC GCATC(5/9)
SfcI 168 CTACAG C^T[GA][CT]AG
SfoI 163 GGCGCC GGC^GCC
SmaI 270 CCCGGG CCC^GGG
TspRI 127 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 270 CCCGGG C^CCGGG
XmaI 270 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 483 CGCG CG^CG
687 CGCG
AfaI 453 GTAC GT^AC
720 GTAC
AlwI 381 GATCC GGATC(4/5)
539 GATCC
AspHI 330 GAGCAC G[AT]GC[AT]^C
478 GAGCTC
BanI 163 GGCGCC G^G[CT][GA]CC
719 GGTACC
BpmI 301 CTGGAG CTGGAG(16/14)
580 CTGGAG
BsiEI 193 CGACCG CG[GA][CT]^CG
603 CGGTCG
BsiHKAI 330 GAGCAC G[AT]GC[AT]^C
478 GAGCTC
BsmAI 26 GTCTC GTCTC(1/5)
90 GTCTC
BsmFI 495 GGGAC GGGAC(10/14)
105 GTCCC
BstF5I 236 CATCC GGATG(2/0)
662 CATCC
BstUI 483 CGCG CG^CG
687 CGCG
BstYI 380 AGATCC [GA]^GATC[CT]
518 AGATCT
DraII 305 AGGACCC [GA]G^G[ACGT]CC[CT]
608 GGGCCCT
EarI 657 CTCTTC CTCTTC(1/4)
327 GAAGAG
EcoO109I 305 AGGACCC [GA]G^G[ACGT]CC[CT]
608 GGGCCCT
FauI 142 GCGGG CCCGC(4/6)
688 GCGGG
FokI 236 CATCC GGATG(9/13)
662 CATCC
HaeII 163 GGCGCC [GA]GCGC^[CT]
277 AGCGCC
Ksp632I 657 CTCTTC CTCTTC(1/4)
327 GAAGAG
MboII 327 GAAGA GAAGA(8/7)
658 TCTTC
MflI 380 AGATCC [GA]^GATC[CT]
518 AGATCT
MvnI 483 CGCG CG^CG
687 CGCG
RsaI 453 GTAC GT^AC
720 GTAC
XhoII 380 AGATCC [GA]^GATC[CT]
518 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI