Restriction map for
pF1KB7969
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 205 GTAGAC GT^[AC][GT]AC
AflIII 317 ACATGT A^C[GA][CT]GT
AlwNI 680 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
BbvI 602 GCTGC GCAGC(8/12)
BciVI 359 GTATCC GTATCC(6/5)
BfaI 229 CTAG C^TAG
BseRI 379 GAGGAG GAGGAG(10/8)
BsmI 822 GAATGC GAATGC(1/-1)
Bsp1407I 804 TGTACA T^GTACA
BspHI 593 TCATGA T^CATGA
BspLU11I 317 ACATGT A^CATGT
BsrGI 804 TGTACA T^GTACA
EcoRI 157 GAATTC G^AATTC
EcoT14I 783 CCTTGG C^C[AT][AT]GG
EcoT22I 824 ATGCAT ATGCA^T
MaeI 229 CTAG C^TAG
MaeII 107 ACGT A^CGT
MaeIII 476 GTTAC ^GT[ACGT]AC
MspA1I 5 CAGCGG C[AC]G^C[GT]G
NdeI 827 CATATG CA^TATG
NsiI 824 ATGCAT ATGCA^T
NspI 317 ACATGT [GA]CATG^[CT]
PleI 331 GACTC GAGTC(4/5)
RcaI 593 TCATGA T^CATGA
SapI 597 GAAGAGC GCTCTTC(1/4)
ScaI 401 AGTACT AGT^ACT
SfaNI 754 GATGC GCATC(5/9)
SspBI 804 TGTACA T^GTACA
SspI 761 AATATT AAT^ATT
StyI 783 CCTTGG C^C[AT][AT]GG
TaqI 555 TCGA T^CGA
TfiI 688 GATTC G^A[AT]TC
TseI 602 GCTGC G^C[AT]GC
TthHB8I 555 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 549 GATCC GGATC(4/5)
682 GGATC
AvaII 435 GGTCC G^G[AT]CC
664 GGACC
BpmI 266 CTCCAG CTGGAG(16/14)
428 CTGGAG
BsaJI 326 CCCAGG C^C[ACGT][ACGT]GG
783 CCTTGG
BsrDI 692 CATTGC GCAATG(2/0)
167 GCAATG
BsrI 363 CCAGT ACTGG(1/-1)
399 CCAGT
BstF5I 797 CATCC GGATG(2/0)
60 GGATG
BstYI 548 AGATCC [GA]^GATC[CT]
682 GGATCT
Cac8I 3 GCCAGC GC[ACGT]^[ACGT]GC
49 GCTTGC
Cfr13I 435 GGTCC G^G[ACGT]CC
664 GGACC
DraI 295 TTTAAA TTT^AAA
367 TTTAAA
Fnu4HI 7 GCGGC GC^[ACGT]GC
602 GCTGC
FokI 797 CATCC GGATG(9/13)
60 GGATG
HaeIII 708 GGCC GG^CC
781 GGCC
HinfI 331 GACTC G^A[ACGT]TC
688 GATTC
ItaI 7 GCGGC GC^[ACGT]GC
602 GCTGC
MflI 548 AGATCC [GA]^GATC[CT]
682 GGATCT
Sau96I 435 GGTCC G^G[ACGT]CC
664 GGACC
SfcI 14 CTGTAG C^T[GA][CT]AG
335 CTATAG
XhoII 548 AGATCC [GA]^GATC[CT]
682 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BssKI BstNI DpnII EarI EcoRII
Ksp632I MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI Tru9I Tsp509I