################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI Bsu36I CelII Cfr10I CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI FspI HpaI KasI KspI MamI MfeI MflI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 608 GGTACC G^GTACC AccI 418 GTATAC GT^[AC][GT]AC AcsI 391 GAATTC [GA]^AATT[CT] AcyI 730 GACGCC G[GA]^CG[CT]C AflIII 1094 ACGTGT A^C[GA][CT]GT ApoI 391 GAATTC [GA]^AATT[CT] AseI 624 ATTAAT AT^TAAT AsnI 624 ATTAAT AT^TAAT Asp718I 608 GGTACC G^GTACC BalI 618 TGGCCA TGG^CCA BbrPI 64 CACGTG CAC^GTG BbsI 106 GAAGAC GAAGAC(2/6) BbvCI 1146 CCTCAGC CCTCAGC(-5/-2) BclI 852 TGATCA T^GATCA BfaI 1066 CTAG C^TAG BmyI 798 GGGCAC G[AGT]GC[ACT]^C BpuAI 106 GAAGAC GAAGAC(2/6) BsaAI 64 CACGTG [CT]AC^GT[GA] BsaHI 730 GACGCC G[GA]^CG[CT]C BseRI 445 CTCCTC GAGGAG(10/8) BsgI 208 GTGCAG GTGCAG(16/14) BsiEI 1152 CGGCCG CG[GA][CT]^CG BsmFI 407 GGGAC GGGAC(10/14) BsmI 867 GCATTC GAATGC(1/-1) Bsp1286I 798 GGGCAC G[AGT]GC[ACT]^C BspDI 262 ATCGAT AT^CGAT Bst1107I 418 GTATAC GTA^TAC BstEII 684 GGTAACC G^GT[ACGT]ACC BstPI 684 GGTAACC G^GT[ACGT]ACC BstXI 818 CCATCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 418 GTATAC GTA^TAC ClaI 262 ATCGAT AT^CGAT DrdI 481 GACAAGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1152 CGGCCG C^GGCCG EclXI 1152 CGGCCG C^GGCCG Eco52I 1152 CGGCCG C^GGCCG EcoNI 21 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 684 GGTAACC G^GT[ACGT]ACC EcoRI 391 GAATTC G^AATTC EcoT14I 396 CCATGG C^C[AT][AT]GG FauI 796 GCGGG CCCGC(4/6) FbaI 852 TGATCA T^GATCA HaeII 30 GGCGCT [GA]GCGC^[CT] HgaI 730 GACGC GACGC(5/10) Hin1I 730 GACGCC G[GA]^CG[CT]C HincII 490 GTCAAC GT[CT]^[GA]AC HindII 490 GTCAAC GT[CT]^[GA]AC HindIII 1279 AAGCTT A^AGCTT KpnI 608 GGTACC GGTAC^C MaeI 1066 CTAG C^TAG MluNI 618 TGGCCA TGG^CCA MscI 618 TGGCCA TGG^CCA NcoI 396 CCATGG C^CATGG NspI 274 GCATGC [GA]CATG^[CT] PmaCI 64 CACGTG CAC^GTG PmlI 64 CACGTG CAC^GTG PpuMI 1157 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 624 ATTAAT AT^TAAT SapI 1134 GAAGAGC GCTCTTC(1/4) SbfI 788 CCTGCAGG CCTGCA^GG SfiI 1144 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 274 GCATGC GCATG^C Sse8387I 788 CCTGCAGG CCTGCA^GG SspI 148 AATATT AAT^ATT StyI 396 CCATGG C^C[AT][AT]GG Tsp45I 1213 GTCAC ^GT[GC]AC XcmI 531 CCACGGTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 82 CGCG CG^CG 823 CGCG AfaI 609 GTAC GT^AC 951 GTAC BanI 608 GGTACC G^G[CT][GA]CC 799 GGCACC BcgI 264 CGATTTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 781 CGAAATGCCTGC BcnI 516 CCGGG CC^[GC]GG 1155 CCGGG BglI 787 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1145 GCCTCAGCGGC BpmI 1231 CTCCAG CTGGAG(16/14) 334 CTGGAG BspMI 381 ACCTGC ACCTGC(4/8) 605 GCAGGT BsrI 371 ACTGG ACTGG(1/-1) 547 ACTGG BstF5I 557 CATCC GGATG(2/0) 819 CATCC BstUI 82 CGCG CG^CG 823 CGCG FokI 557 CATCC GGATG(9/13) 819 CATCC HapII 516 CCGG C^CGG 1155 CCGG HpaII 516 CCGG C^CGG 1155 CCGG MaeIII 685 GTAAC ^GT[ACGT]AC 1213 GTCAC MspI 516 CCGG C^CGG 1155 CCGG MvnI 82 CGCG CG^CG 823 CGCG NciI 516 CCGGG CC^[GC]GG 1155 CCGGG RsaI 609 GTAC GT^AC 951 GTAC SfaNI 814 GATGC GCATC(5/9) 75 GCATC TfiI 705 GATTC G^A[AT]TC 882 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I