##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI
Bsu36I CelII Cfr10I CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoRV
EcoT22I FseI FspI HpaI KasI
KspI MamI MfeI MflI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 608 GGTACC G^GTACC
AccI 418 GTATAC GT^[AC][GT]AC
AcsI 391 GAATTC [GA]^AATT[CT]
AcyI 730 GACGCC G[GA]^CG[CT]C
AflIII 1094 ACGTGT A^C[GA][CT]GT
ApoI 391 GAATTC [GA]^AATT[CT]
AseI 624 ATTAAT AT^TAAT
AsnI 624 ATTAAT AT^TAAT
Asp718I 608 GGTACC G^GTACC
BalI 618 TGGCCA TGG^CCA
BbrPI 64 CACGTG CAC^GTG
BbsI 106 GAAGAC GAAGAC(2/6)
BbvCI 1146 CCTCAGC CCTCAGC(-5/-2)
BclI 852 TGATCA T^GATCA
BfaI 1066 CTAG C^TAG
BmyI 798 GGGCAC G[AGT]GC[ACT]^C
BpuAI 106 GAAGAC GAAGAC(2/6)
BsaAI 64 CACGTG [CT]AC^GT[GA]
BsaHI 730 GACGCC G[GA]^CG[CT]C
BseRI 445 CTCCTC GAGGAG(10/8)
BsgI 208 GTGCAG GTGCAG(16/14)
BsiEI 1152 CGGCCG CG[GA][CT]^CG
BsmFI 407 GGGAC GGGAC(10/14)
BsmI 867 GCATTC GAATGC(1/-1)
Bsp1286I 798 GGGCAC G[AGT]GC[ACT]^C
BspDI 262 ATCGAT AT^CGAT
Bst1107I 418 GTATAC GTA^TAC
BstEII 684 GGTAACC G^GT[ACGT]ACC
BstPI 684 GGTAACC G^GT[ACGT]ACC
BstXI 818 CCATCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 418 GTATAC GTA^TAC
ClaI 262 ATCGAT AT^CGAT
DrdI 481 GACAAGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1152 CGGCCG C^GGCCG
EclXI 1152 CGGCCG C^GGCCG
Eco52I 1152 CGGCCG C^GGCCG
EcoNI 21 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 684 GGTAACC G^GT[ACGT]ACC
EcoRI 391 GAATTC G^AATTC
EcoT14I 396 CCATGG C^C[AT][AT]GG
FauI 796 GCGGG CCCGC(4/6)
FbaI 852 TGATCA T^GATCA
HaeII 30 GGCGCT [GA]GCGC^[CT]
HgaI 730 GACGC GACGC(5/10)
Hin1I 730 GACGCC G[GA]^CG[CT]C
HincII 490 GTCAAC GT[CT]^[GA]AC
HindII 490 GTCAAC GT[CT]^[GA]AC
HindIII 1279 AAGCTT A^AGCTT
KpnI 608 GGTACC GGTAC^C
MaeI 1066 CTAG C^TAG
MluNI 618 TGGCCA TGG^CCA
MscI 618 TGGCCA TGG^CCA
NcoI 396 CCATGG C^CATGG
NspI 274 GCATGC [GA]CATG^[CT]
PmaCI 64 CACGTG CAC^GTG
PmlI 64 CACGTG CAC^GTG
PpuMI 1157 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 624 ATTAAT AT^TAAT
SapI 1134 GAAGAGC GCTCTTC(1/4)
SbfI 788 CCTGCAGG CCTGCA^GG
SfiI 1144 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 274 GCATGC GCATG^C
Sse8387I 788 CCTGCAGG CCTGCA^GG
SspI 148 AATATT AAT^ATT
StyI 396 CCATGG C^C[AT][AT]GG
Tsp45I 1213 GTCAC ^GT[GC]AC
XcmI 531 CCACGGTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 82 CGCG CG^CG
823 CGCG
AfaI 609 GTAC GT^AC
951 GTAC
BanI 608 GGTACC G^G[CT][GA]CC
799 GGCACC
BcgI 264 CGATTTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
781 CGAAATGCCTGC
BcnI 516 CCGGG CC^[GC]GG
1155 CCGGG
BglI 787 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1145 GCCTCAGCGGC
BpmI 1231 CTCCAG CTGGAG(16/14)
334 CTGGAG
BspMI 381 ACCTGC ACCTGC(4/8)
605 GCAGGT
BsrI 371 ACTGG ACTGG(1/-1)
547 ACTGG
BstF5I 557 CATCC GGATG(2/0)
819 CATCC
BstUI 82 CGCG CG^CG
823 CGCG
FokI 557 CATCC GGATG(9/13)
819 CATCC
HapII 516 CCGG C^CGG
1155 CCGG
HpaII 516 CCGG C^CGG
1155 CCGG
MaeIII 685 GTAAC ^GT[ACGT]AC
1213 GTCAC
MspI 516 CCGG C^CGG
1155 CCGG
MvnI 82 CGCG CG^CG
823 CGCG
NciI 516 CCGGG CC^[GC]GG
1155 CCGGG
RsaI 609 GTAC GT^AC
951 GTAC
SfaNI 814 GATGC GCATC(5/9)
75 GCATC
TfiI 705 GATTC G^A[AT]TC
882 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BsaJI BsiYI BslI BsmAI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII Fnu4HI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I