Restriction map for
pF1KB5808
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrBI BssHII
BssSI ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp509I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 1097 GGCGCC G[GA]^CG[CT]C
AlwNI 949 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1080 CCTGAGG CC^T[ACGT]AGG
ApaI 913 GGGCCC GGGCC^C
Asp700I 444 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 925 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 251 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BbeI 1097 GGCGCC GGCGC^C
BbrPI 372 CACGTG CAC^GTG
BglI 614 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 372 CACGTG [CT]AC^GT[GA]
BsaHI 1097 GGCGCC G[GA]^CG[CT]C
BsoBI 251 CTCGGG C^[CT]CG[GA]G
Bsp1407I 368 TGTACA T^GTACA
BspLU11I 1121 ACATGT A^CATGT
BspMI 67 GCAGGT ACCTGC(4/8)
BsrGI 368 TGTACA T^GTACA
Bst1107I 627 GTATAC GTA^TAC
BstAPI 948 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 451 TTCGAA TT^CGAA
BstXI 164 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 627 GTATAC GTA^TAC
Bsu36I 1080 CCTGAGG CC^T[ACGT]AGG
DraIII 652 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 559 GAAGAG CTCTTC(1/4)
Eco81I 1080 CCTGAGG CC^T[ACGT]AGG
EcoNI 159 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 805 GATATC GAT^ATC
Hin1I 1097 GGCGCC G[GA]^CG[CT]C
KasI 1097 GGCGCC G^GCGCC
Ksp632I 559 GAAGAG CTCTTC(1/4)
MaeII 373 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 1231 TTAA T^TAA
MslI 1294 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1097 GGCGCC GG^CGCC
NspV 451 TTCGAA TT^CGAA
PflFI 925 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 372 CACGTG CAC^GTG
PmlI 372 CACGTG CAC^GTG
PstI 1057 CTGCAG CTGCA^G
SacI 562 GAGCTC GAGCT^C
SapI 559 GAAGAGC GCTCTTC(1/4)
ScaI 428 AGTACT AGT^ACT
SfoI 1097 GGCGCC GGC^GCC
SfuI 451 TTCGAA TT^CGAA
SgrAI 1099 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 368 TGTACA T^GTACA
Tru9I 1231 TTAA T^TAA
Tth111I 925 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 444 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 256 GTCTAC GT^[AC][GT]AC
627 GTATAC
AccII 382 CGCG CG^CG
655 CGCG
AlwI 49 GGATC GGATC(4/5)
666 GGATC
AspHI 198 GAGCAC G[AT]GC[AT]^C
562 GAGCTC
BsaI 1016 GAGACC GGTCTC(1/5)
104 GGTCTC
BsaWI 488 ACCGGA [AT]^CCGG[AT]
884 ACCGGA
BsiHKAI 198 GAGCAC G[AT]GC[AT]^C
562 GAGCTC
BsrDI 765 CATTGC GCAATG(2/0)
278 GCAATG
BsrI 426 CCAGT ACTGG(1/-1)
641 CCAGT
BstEII 408 GGTGACC G^GT[ACGT]ACC
524 GGTCACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
524 GGTCACC
BstUI 382 CGCG CG^CG
655 CGCG
BstYI 49 GGATCT [GA]^GATC[CT]
666 GGATCT
CfoI 995 GCGC GCG^C
1098 GCGC
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
524 GGTCACC
HaeII 994 AGCGCC [GA]GCGC^[CT]
1097 GGCGCC
HhaI 995 GCGC GCG^C
1098 GCGC
HinP1I 995 GCGC G^CGC
1098 GCGC
MflI 49 GGATCT [GA]^GATC[CT]
666 GGATCT
MspA1I 616 CAGCTG C[AC]G^C[GT]G
661 CAGCTG
MvnI 382 CGCG CG^CG
655 CGCG
NspI 461 GCATGT [GA]CATG^[CT]
1121 ACATGT
PleI 20 GACTC GAGTC(4/5)
1273 GACTC
PvuII 616 CAGCTG CAG^CTG
661 CAGCTG
SfcI 439 CTACAG C^T[GA][CT]AG
1057 CTGCAG
TaqI 116 TCGA T^CGA
452 TCGA
TfiI 417 GAATC G^A[AT]TC
1209 GATTC
TthHB8I 116 TCGA T^CGA
452 TCGA
XhoII 49 GGATCT [GA]^GATC[CT]
666 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI