Restriction map for
pF1KB5811
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
Alw44I AlwNI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstXI BstZ17I CelII CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NarI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SfaNI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 300 GTCTAC GT^[AC][GT]AC
AcsI 143 AAATTC [GA]^AATT[CT]
AflII 1137 CTTAAG C^TTAAG
AhdI 1390 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1368 CCTGAGG CC^T[ACGT]AGG
Aor51HI 773 AGCGCT AGC^GCT
ApoI 143 AAATTC [GA]^AATT[CT]
AspEI 1390 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 427 GTGCTC G[AT]GC[AT]^C
AvaI 1376 CCCGAG C^[CT]CG[GA]G
BanII 409 GAGCCC G[GA]GC[CT]^C
BfrI 1137 CTTAAG C^TTAAG
BglII 695 AGATCT A^GATCT
BsaI 1306 GAGACC GGTCTC(1/5)
BsiHKAI 427 GTGCTC G[AT]GC[AT]^C
BsoBI 1376 CCCGAG C^[CT]CG[GA]G
BspDI 364 ATCGAT AT^CGAT
BsrDI 1253 GCAATG GCAATG(2/0)
BsrFI 80 GCCGGC [GA]^CCGG[CT]
BstBI 469 TTCGAA TT^CGAA
BstEII 1416 GGTGACC G^GT[ACGT]ACC
BstPI 1416 GGTGACC G^GT[ACGT]ACC
Bsu36I 1368 CCTGAGG CC^T[ACGT]AGG
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
ClaI 364 ATCGAT AT^CGAT
Eam1105I 1390 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 773 AGCGCT AGC^GCT
Eco81I 1368 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1416 GGTGACC G^GT[ACGT]ACC
EcoT14I 1062 CCAAGG C^C[AT][AT]GG
HincII 898 GTTGAC GT[CT]^[GA]AC
HindII 898 GTTGAC GT[CT]^[GA]AC
MfeI 1148 CAATTG C^AATTG
MslI 1344 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1148 CAATTG C^AATTG
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NspV 469 TTCGAA TT^CGAA
SapI 1245 GAAGAGC GCTCTTC(1/4)
ScaI 1286 AGTACT AGT^ACT
SexAI 902 ACCTGGT A^CC[AT]GGT
SfuI 469 TTCGAA TT^CGAA
StyI 1062 CCAAGG C^C[AT][AT]GG
XbaI 43 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 344 GACGTC GACGT^C
1128 GACGTC
AcyI 344 GACGTC G[GA]^CG[CT]C
1128 GACGTC
AspI 214 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACCTGGTC
BalI 185 TGGCCA TGG^CCA
1281 TGGCCA
BbsI 272 GAAGAC GAAGAC(2/6)
1125 GAAGAC
BcnI 264 CCCGG CC^[GC]GG
734 CCGGG
BfaI 44 CTAG C^TAG
1216 CTAG
BpuAI 272 GAAGAC GAAGAC(2/6)
1125 GAAGAC
BsaHI 344 GACGTC G[GA]^CG[CT]C
1128 GACGTC
EarI 1290 CTCTTC CTCTTC(1/4)
1245 GAAGAG
EcoNI 903 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1133 CCTTCTTAAGG
HgaI 275 GACGC GACGC(5/10)
315 GACGC
Hin1I 344 GACGTC G[GA]^CG[CT]C
1128 GACGTC
Ksp632I 1290 CTCTTC CTCTTC(1/4)
1245 GAAGAG
MaeI 44 CTAG C^TAG
1216 CTAG
MluNI 185 TGGCCA TGG^CCA
1281 TGGCCA
MscI 185 TGGCCA TGG^CCA
1281 TGGCCA
NciI 264 CCCGG CC^[GC]GG
734 CCGGG
NspI 557 GCATGC [GA]CATG^[CT]
1325 GCATGC
PflFI 214 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACCTGGTC
PshAI 275 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
732 GACCGGGGTC
SmlI 334 CTTGAG C^T[CT][GA]AG
1137 CTTAAG
SphI 557 GCATGC GCATG^C
1325 GCATGC
Tth111I 214 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII